Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae)
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2013/206581 http://hdl.handle.net/11449/74820 |
Resumo: | The crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 μg/mL), methylene chloride (IC 50 = 5.9 g/mL), ethyl acetate (IC50 = 8.0 g/mL), 4-nerolidylcatechol (IC50 = 8.6 g/mL), and the sterol fractions (IC50 > 12.5 μg/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 μg/mL. 4-Nerolidylcatechol (IC50 = 0.4 μg/mL) and methylene chloride fraction (IC50 = 2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective. © 2013 Andrey P. Lopes et al. |
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Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae)4 nerolidylcatecholacetic acid ethyl esteralcoholcatechol derivativedichloromethaneplant extractPothomorphe umbellata plant extractsterolunclassified drugantioxidant activitycontrolled studycytotoxicitydrug solubilitydrug stabilityhumanhuman cellmedicinal plantoxidationPiperaceaeplant leafPothomorphe umbellataThe crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 μg/mL), methylene chloride (IC 50 = 5.9 g/mL), ethyl acetate (IC50 = 8.0 g/mL), 4-nerolidylcatechol (IC50 = 8.6 g/mL), and the sterol fractions (IC50 > 12.5 μg/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 μg/mL. 4-Nerolidylcatechol (IC50 = 0.4 μg/mL) and methylene chloride fraction (IC50 = 2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective. © 2013 Andrey P. Lopes et al.Departamento de Ciências Exatas e da Terra Instituto de Ciências Ambientais Universidade Federal de São Paulo, São Nicolau Street 210, 09972-270 Diadema, SPNational Center for Natural Products Research Research Institute of Pharmaceutical Sciences University of Mississippi, Oxford, MS 38677Laboratório de Pesquisa em Fármacos Universidade Federal Do Amapá, 68902-280 Macapá, APDepartamento de Fonoaudiologia Faculdade de Filosofia e Ciências Universidade Estadual Paulista, 17525-900 Marília, SPDepartamento de Ciências Farmacêuticas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, 14040-903 Ribeirão Preto, SPDepartamento de Fonoaudiologia Faculdade de Filosofia e Ciências Universidade Estadual Paulista, 17525-900 Marília, SPUniversidade Federal de São Paulo (UNIFESP)University of MississippiUniversidade Federal do Amapá (UNIFAP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Lopes, Andrey P.Bagatela, Bianca S.Rosa, Paulo C. P.Nanayakkara, Dhammika N. P.Carlos Tavares Carvalho, JoséMaistro, Edson Luis [UNESP]Bastos, Jairo K.Perazzo, Fábio F.2014-05-27T11:28:39Z2014-05-27T11:28:39Z2013-03-11info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2013/206581BioMed Research International, v. 2013.2314-61332314-6141http://hdl.handle.net/11449/7482010.1155/2013/206581WOS:0003136868000012-s2.0-848745868812-s2.0-84874586881.pdf47875216130383150000-0003-0757-7876Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioMed Research International2.5830,9350,935info:eu-repo/semantics/openAccess2023-11-25T06:19:20Zoai:repositorio.unesp.br:11449/74820Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-25T06:19:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) |
title |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) |
spellingShingle |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) Lopes, Andrey P. 4 nerolidylcatechol acetic acid ethyl ester alcohol catechol derivative dichloromethane plant extract Pothomorphe umbellata plant extract sterol unclassified drug antioxidant activity controlled study cytotoxicity drug solubility drug stability human human cell medicinal plant oxidation Piperaceae plant leaf Pothomorphe umbellata |
title_short |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) |
title_full |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) |
title_fullStr |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) |
title_full_unstemmed |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) |
title_sort |
Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae) |
author |
Lopes, Andrey P. |
author_facet |
Lopes, Andrey P. Bagatela, Bianca S. Rosa, Paulo C. P. Nanayakkara, Dhammika N. P. Carlos Tavares Carvalho, José Maistro, Edson Luis [UNESP] Bastos, Jairo K. Perazzo, Fábio F. |
author_role |
author |
author2 |
Bagatela, Bianca S. Rosa, Paulo C. P. Nanayakkara, Dhammika N. P. Carlos Tavares Carvalho, José Maistro, Edson Luis [UNESP] Bastos, Jairo K. Perazzo, Fábio F. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Paulo (UNIFESP) University of Mississippi Universidade Federal do Amapá (UNIFAP) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Lopes, Andrey P. Bagatela, Bianca S. Rosa, Paulo C. P. Nanayakkara, Dhammika N. P. Carlos Tavares Carvalho, José Maistro, Edson Luis [UNESP] Bastos, Jairo K. Perazzo, Fábio F. |
dc.subject.por.fl_str_mv |
4 nerolidylcatechol acetic acid ethyl ester alcohol catechol derivative dichloromethane plant extract Pothomorphe umbellata plant extract sterol unclassified drug antioxidant activity controlled study cytotoxicity drug solubility drug stability human human cell medicinal plant oxidation Piperaceae plant leaf Pothomorphe umbellata |
topic |
4 nerolidylcatechol acetic acid ethyl ester alcohol catechol derivative dichloromethane plant extract Pothomorphe umbellata plant extract sterol unclassified drug antioxidant activity controlled study cytotoxicity drug solubility drug stability human human cell medicinal plant oxidation Piperaceae plant leaf Pothomorphe umbellata |
description |
The crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 μg/mL), methylene chloride (IC 50 = 5.9 g/mL), ethyl acetate (IC50 = 8.0 g/mL), 4-nerolidylcatechol (IC50 = 8.6 g/mL), and the sterol fractions (IC50 > 12.5 μg/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 μg/mL. 4-Nerolidylcatechol (IC50 = 0.4 μg/mL) and methylene chloride fraction (IC50 = 2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective. © 2013 Andrey P. Lopes et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-11 2014-05-27T11:28:39Z 2014-05-27T11:28:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2013/206581 BioMed Research International, v. 2013. 2314-6133 2314-6141 http://hdl.handle.net/11449/74820 10.1155/2013/206581 WOS:000313686800001 2-s2.0-84874586881 2-s2.0-84874586881.pdf 4787521613038315 0000-0003-0757-7876 |
url |
http://dx.doi.org/10.1155/2013/206581 http://hdl.handle.net/11449/74820 |
identifier_str_mv |
BioMed Research International, v. 2013. 2314-6133 2314-6141 10.1155/2013/206581 WOS:000313686800001 2-s2.0-84874586881 2-s2.0-84874586881.pdf 4787521613038315 0000-0003-0757-7876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BioMed Research International 2.583 0,935 0,935 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965070114947072 |