Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.lfs.2020.118693 http://hdl.handle.net/11449/208123 |
Resumo: | Aims: Because an adequate protein supply is detrimental for the maintenance of folliculogenesis and ovulation, we evaluated the impact of maternal low protein diet on nutritional parameters, estrous cycle, ovarian histomorphometry, and on the expression of metabolic and survival signaling molecules in different follicular stages. Main methods: Twenty Wistar pregnant rats were divided into two groups: the normoprotein (NP) group, composed of animals that received 17% protein, and a low-protein (LP) group, composed of animals that received 6% protein during gestation and lactation period. After weaning, female rats were fed with standard diet until the 120-days-old. Key findings: LP animals showed reduced body mass index, total body weight, energy intake, feed efficiency, and visceral fat. The ovarian tissue presented vascular congestion and fat accumulation in the medulla, followed by a significant reduction in the amount of primordial and primary follicles. In addition, the number of atretic follicles was higher in LP than in NP animals. Maternal undernutrition also resulted in increased levels of estradiol (E2) and progesterone (P4) while testosterone (T) was unchanged in the offspring. Although discrete changes in p38MAPK and in PI3K-AKT-mTOR immunostaining were observed in the ovarian follicles and corpus luteum in LP, no differences were found at their protein levels. Significance: Maternal protein restriction alters estrous cycle and histomorphometry of the offspring's ovary without changing the levels of intracellular regulatory molecules in adulthood. These morphofunctional changes may alter reproductive performance in female offspring, highlighting maternal dietary conditions as an important factor for offspring reproductive health. |
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Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovariesAKTDOHaDEstrous cycleLow-protein dietMAPKMaternal restrictionmTOROvarian tissuePI3KAims: Because an adequate protein supply is detrimental for the maintenance of folliculogenesis and ovulation, we evaluated the impact of maternal low protein diet on nutritional parameters, estrous cycle, ovarian histomorphometry, and on the expression of metabolic and survival signaling molecules in different follicular stages. Main methods: Twenty Wistar pregnant rats were divided into two groups: the normoprotein (NP) group, composed of animals that received 17% protein, and a low-protein (LP) group, composed of animals that received 6% protein during gestation and lactation period. After weaning, female rats were fed with standard diet until the 120-days-old. Key findings: LP animals showed reduced body mass index, total body weight, energy intake, feed efficiency, and visceral fat. The ovarian tissue presented vascular congestion and fat accumulation in the medulla, followed by a significant reduction in the amount of primordial and primary follicles. In addition, the number of atretic follicles was higher in LP than in NP animals. Maternal undernutrition also resulted in increased levels of estradiol (E2) and progesterone (P4) while testosterone (T) was unchanged in the offspring. Although discrete changes in p38MAPK and in PI3K-AKT-mTOR immunostaining were observed in the ovarian follicles and corpus luteum in LP, no differences were found at their protein levels. Significance: Maternal protein restriction alters estrous cycle and histomorphometry of the offspring's ovary without changing the levels of intracellular regulatory molecules in adulthood. These morphofunctional changes may alter reproductive performance in female offspring, highlighting maternal dietary conditions as an important factor for offspring reproductive health.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculty of Medicine of Botucatu São Paulo State University-UNESPDepartment of Structural and Functional Biology Institute of Biosciences (IBB) São Paulo State University-UNESPFaculty of Medicine of Botucatu São Paulo State University-UNESPDepartment of Structural and Functional Biology Institute of Biosciences (IBB) São Paulo State University-UNESPFAPESP: 2018/02224-7Universidade Estadual Paulista (Unesp)Côrtes, Lucas Silva [UNESP]Silveira, Henrique Spaulonci [UNESP]Lupi, Luiz Antonio [UNESP]de Mello Santos, Talita [UNESP]Cavariani, Marilia Martins [UNESP]Domeniconi, Raquel Fantin [UNESP]Gaiotte, Letícia Barbosa [UNESP]de Morais Oliveira, Diego Augusto [UNESP]Justulin, Luis Antonio [UNESP]de Almeida Chuffa, Luiz Gustavo [UNESP]2021-06-25T11:06:43Z2021-06-25T11:06:43Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.lfs.2020.118693Life Sciences, v. 264.1879-06310024-3205http://hdl.handle.net/11449/20812310.1016/j.lfs.2020.1186932-s2.0-85095818451Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2021-10-23T18:56:44Zoai:repositorio.unesp.br:11449/208123Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T18:56:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries |
title |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries |
spellingShingle |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries Côrtes, Lucas Silva [UNESP] AKT DOHaD Estrous cycle Low-protein diet MAPK Maternal restriction mTOR Ovarian tissue PI3K |
title_short |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries |
title_full |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries |
title_fullStr |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries |
title_full_unstemmed |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries |
title_sort |
Maternal protein restriction impairs nutrition and ovarian histomorphometry without changing p38MAPK and PI3K-AKT-mTOR signaling in adult rat ovaries |
author |
Côrtes, Lucas Silva [UNESP] |
author_facet |
Côrtes, Lucas Silva [UNESP] Silveira, Henrique Spaulonci [UNESP] Lupi, Luiz Antonio [UNESP] de Mello Santos, Talita [UNESP] Cavariani, Marilia Martins [UNESP] Domeniconi, Raquel Fantin [UNESP] Gaiotte, Letícia Barbosa [UNESP] de Morais Oliveira, Diego Augusto [UNESP] Justulin, Luis Antonio [UNESP] de Almeida Chuffa, Luiz Gustavo [UNESP] |
author_role |
author |
author2 |
Silveira, Henrique Spaulonci [UNESP] Lupi, Luiz Antonio [UNESP] de Mello Santos, Talita [UNESP] Cavariani, Marilia Martins [UNESP] Domeniconi, Raquel Fantin [UNESP] Gaiotte, Letícia Barbosa [UNESP] de Morais Oliveira, Diego Augusto [UNESP] Justulin, Luis Antonio [UNESP] de Almeida Chuffa, Luiz Gustavo [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Côrtes, Lucas Silva [UNESP] Silveira, Henrique Spaulonci [UNESP] Lupi, Luiz Antonio [UNESP] de Mello Santos, Talita [UNESP] Cavariani, Marilia Martins [UNESP] Domeniconi, Raquel Fantin [UNESP] Gaiotte, Letícia Barbosa [UNESP] de Morais Oliveira, Diego Augusto [UNESP] Justulin, Luis Antonio [UNESP] de Almeida Chuffa, Luiz Gustavo [UNESP] |
dc.subject.por.fl_str_mv |
AKT DOHaD Estrous cycle Low-protein diet MAPK Maternal restriction mTOR Ovarian tissue PI3K |
topic |
AKT DOHaD Estrous cycle Low-protein diet MAPK Maternal restriction mTOR Ovarian tissue PI3K |
description |
Aims: Because an adequate protein supply is detrimental for the maintenance of folliculogenesis and ovulation, we evaluated the impact of maternal low protein diet on nutritional parameters, estrous cycle, ovarian histomorphometry, and on the expression of metabolic and survival signaling molecules in different follicular stages. Main methods: Twenty Wistar pregnant rats were divided into two groups: the normoprotein (NP) group, composed of animals that received 17% protein, and a low-protein (LP) group, composed of animals that received 6% protein during gestation and lactation period. After weaning, female rats were fed with standard diet until the 120-days-old. Key findings: LP animals showed reduced body mass index, total body weight, energy intake, feed efficiency, and visceral fat. The ovarian tissue presented vascular congestion and fat accumulation in the medulla, followed by a significant reduction in the amount of primordial and primary follicles. In addition, the number of atretic follicles was higher in LP than in NP animals. Maternal undernutrition also resulted in increased levels of estradiol (E2) and progesterone (P4) while testosterone (T) was unchanged in the offspring. Although discrete changes in p38MAPK and in PI3K-AKT-mTOR immunostaining were observed in the ovarian follicles and corpus luteum in LP, no differences were found at their protein levels. Significance: Maternal protein restriction alters estrous cycle and histomorphometry of the offspring's ovary without changing the levels of intracellular regulatory molecules in adulthood. These morphofunctional changes may alter reproductive performance in female offspring, highlighting maternal dietary conditions as an important factor for offspring reproductive health. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:06:43Z 2021-06-25T11:06:43Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.lfs.2020.118693 Life Sciences, v. 264. 1879-0631 0024-3205 http://hdl.handle.net/11449/208123 10.1016/j.lfs.2020.118693 2-s2.0-85095818451 |
url |
http://dx.doi.org/10.1016/j.lfs.2020.118693 http://hdl.handle.net/11449/208123 |
identifier_str_mv |
Life Sciences, v. 264. 1879-0631 0024-3205 10.1016/j.lfs.2020.118693 2-s2.0-85095818451 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Life Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789356065292288 |