Siloxane-modified bacterial cellulose as a promising platform for cell culture
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10570-022-04872-4 http://hdl.handle.net/11449/249277 |
Resumo: | Cellulose is a versatile tunable material that finds application in the biomedical field as substrate for cell culture. However, few studies have been done to explore the use of bacterial cellulose (BC), a naturally occurring nanofibrillar material with distinct properties, as a platform to produce such device. In the present work, BC membranes have been functionalized with thiol functional group (SH) through silanization reaction with (3-mercaptopropyl)trimethoxysilane (MPTMS) under different conditions, in order to obtain a platform with improved cell adhesion. The efficiency of BC surface modification with MPTMS was evaluated by using acid and base catalyzed reactions, and two different drying methods: at room temperature of 28 °C and at 120 °C as curing temperature. The results of the set of analyses performed—ATR-FTIR, TGA, elemental analysis,13C NMR, contact angle and SEM—indicate that BC surface functionalization was efficient, regardless the drying process. The MPTMS-modified platforms exhibited sulfur content of 3–5 times higher than native BC. The performed biological assay with fibroblast GM07492 human cells revealed that adhesion of cells to the BC surface depends not only on the functional group present at the matrix but also on surface wettability. Graphical abstract: [Figure not available: see fulltext.]Schematic illustration of a siloxane-modified bacterial cellulose as platform for cell culture. |
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Siloxane-modified bacterial cellulose as a promising platform for cell cultureBacterial celluloseCell cultureMercaptoPlatformSiloxaneCellulose is a versatile tunable material that finds application in the biomedical field as substrate for cell culture. However, few studies have been done to explore the use of bacterial cellulose (BC), a naturally occurring nanofibrillar material with distinct properties, as a platform to produce such device. In the present work, BC membranes have been functionalized with thiol functional group (SH) through silanization reaction with (3-mercaptopropyl)trimethoxysilane (MPTMS) under different conditions, in order to obtain a platform with improved cell adhesion. The efficiency of BC surface modification with MPTMS was evaluated by using acid and base catalyzed reactions, and two different drying methods: at room temperature of 28 °C and at 120 °C as curing temperature. The results of the set of analyses performed—ATR-FTIR, TGA, elemental analysis,13C NMR, contact angle and SEM—indicate that BC surface functionalization was efficient, regardless the drying process. The MPTMS-modified platforms exhibited sulfur content of 3–5 times higher than native BC. The performed biological assay with fibroblast GM07492 human cells revealed that adhesion of cells to the BC surface depends not only on the functional group present at the matrix but also on surface wettability. Graphical abstract: [Figure not available: see fulltext.]Schematic illustration of a siloxane-modified bacterial cellulose as platform for cell culture.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)University of Araraquara - UNIARA, Rua Carlos Gomes 1217, SPDepartment of Chemistry Federal University of São Carlos, P.O. Box 676, SPTechMiP Análises e Soluções Inteligentes LTDA, Av. Jorge Fernandes de Mattos 311, SPMedical School Sao Paulo State University Julio de Mesquita Filho, Av. Prof. Mário Rubens Guimarães Montenegro, s/n - UNESP - Campus de BotucatuMedical School Sao Paulo State University Julio de Mesquita Filho, Av. Prof. Mário Rubens Guimarães Montenegro, s/n - UNESP - Campus de BotucatuFAPESP: 2018/23853-2FAPESP: 2019/12711-5FAPESP: 2020/05163-9University of Araraquara - UNIARAUniversidade Federal de São Carlos (UFSCar)TechMiP Análises e Soluções Inteligentes LTDAUniversidade Estadual Paulista (UNESP)Claro, Amanda MariaDo Amaral, Nayara CavichiolliColturato, Vitória Maria MedalhaAleixo, Nadia AndradePaiva, RobertCruz, Sandra AndreaMonteiro, Gustavo ClaroDe Carvalho, Gustavo Senra GonçalvesNogueira, Flávia Aparecida ResendeDeffune, Elenice [UNESP]Iemma, Mônica Rosas da CostaBarud, Hernane da Silva2023-07-29T14:52:48Z2023-07-29T14:52:48Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9597-9608http://dx.doi.org/10.1007/s10570-022-04872-4Cellulose, v. 29, n. 18, p. 9597-9608, 2022.1572-882X0969-0239http://hdl.handle.net/11449/24927710.1007/s10570-022-04872-42-s2.0-85140061405Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCelluloseinfo:eu-repo/semantics/openAccess2023-07-29T14:52:48Zoai:repositorio.unesp.br:11449/249277Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T14:52:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Siloxane-modified bacterial cellulose as a promising platform for cell culture |
title |
Siloxane-modified bacterial cellulose as a promising platform for cell culture |
spellingShingle |
Siloxane-modified bacterial cellulose as a promising platform for cell culture Claro, Amanda Maria Bacterial cellulose Cell culture Mercapto Platform Siloxane |
title_short |
Siloxane-modified bacterial cellulose as a promising platform for cell culture |
title_full |
Siloxane-modified bacterial cellulose as a promising platform for cell culture |
title_fullStr |
Siloxane-modified bacterial cellulose as a promising platform for cell culture |
title_full_unstemmed |
Siloxane-modified bacterial cellulose as a promising platform for cell culture |
title_sort |
Siloxane-modified bacterial cellulose as a promising platform for cell culture |
author |
Claro, Amanda Maria |
author_facet |
Claro, Amanda Maria Do Amaral, Nayara Cavichiolli Colturato, Vitória Maria Medalha Aleixo, Nadia Andrade Paiva, Robert Cruz, Sandra Andrea Monteiro, Gustavo Claro De Carvalho, Gustavo Senra Gonçalves Nogueira, Flávia Aparecida Resende Deffune, Elenice [UNESP] Iemma, Mônica Rosas da Costa Barud, Hernane da Silva |
author_role |
author |
author2 |
Do Amaral, Nayara Cavichiolli Colturato, Vitória Maria Medalha Aleixo, Nadia Andrade Paiva, Robert Cruz, Sandra Andrea Monteiro, Gustavo Claro De Carvalho, Gustavo Senra Gonçalves Nogueira, Flávia Aparecida Resende Deffune, Elenice [UNESP] Iemma, Mônica Rosas da Costa Barud, Hernane da Silva |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Araraquara - UNIARA Universidade Federal de São Carlos (UFSCar) TechMiP Análises e Soluções Inteligentes LTDA Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Claro, Amanda Maria Do Amaral, Nayara Cavichiolli Colturato, Vitória Maria Medalha Aleixo, Nadia Andrade Paiva, Robert Cruz, Sandra Andrea Monteiro, Gustavo Claro De Carvalho, Gustavo Senra Gonçalves Nogueira, Flávia Aparecida Resende Deffune, Elenice [UNESP] Iemma, Mônica Rosas da Costa Barud, Hernane da Silva |
dc.subject.por.fl_str_mv |
Bacterial cellulose Cell culture Mercapto Platform Siloxane |
topic |
Bacterial cellulose Cell culture Mercapto Platform Siloxane |
description |
Cellulose is a versatile tunable material that finds application in the biomedical field as substrate for cell culture. However, few studies have been done to explore the use of bacterial cellulose (BC), a naturally occurring nanofibrillar material with distinct properties, as a platform to produce such device. In the present work, BC membranes have been functionalized with thiol functional group (SH) through silanization reaction with (3-mercaptopropyl)trimethoxysilane (MPTMS) under different conditions, in order to obtain a platform with improved cell adhesion. The efficiency of BC surface modification with MPTMS was evaluated by using acid and base catalyzed reactions, and two different drying methods: at room temperature of 28 °C and at 120 °C as curing temperature. The results of the set of analyses performed—ATR-FTIR, TGA, elemental analysis,13C NMR, contact angle and SEM—indicate that BC surface functionalization was efficient, regardless the drying process. The MPTMS-modified platforms exhibited sulfur content of 3–5 times higher than native BC. The performed biological assay with fibroblast GM07492 human cells revealed that adhesion of cells to the BC surface depends not only on the functional group present at the matrix but also on surface wettability. Graphical abstract: [Figure not available: see fulltext.]Schematic illustration of a siloxane-modified bacterial cellulose as platform for cell culture. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-07-29T14:52:48Z 2023-07-29T14:52:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10570-022-04872-4 Cellulose, v. 29, n. 18, p. 9597-9608, 2022. 1572-882X 0969-0239 http://hdl.handle.net/11449/249277 10.1007/s10570-022-04872-4 2-s2.0-85140061405 |
url |
http://dx.doi.org/10.1007/s10570-022-04872-4 http://hdl.handle.net/11449/249277 |
identifier_str_mv |
Cellulose, v. 29, n. 18, p. 9597-9608, 2022. 1572-882X 0969-0239 10.1007/s10570-022-04872-4 2-s2.0-85140061405 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellulose |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9597-9608 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964893301964800 |