Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2019.00614 http://hdl.handle.net/11449/189202 |
Resumo: | This study investigated the long-term effects (13 months) of encapsulated nitrate supplementation (ENS) on enteric methane emissions, rumen fermentation parameters, ruminal bacteria, and diversity of archaea in grazing beef cattle. We used a total of thirty-two Nellore steers (initial BW of 197 ± 15.3 kg), 12 of which were fitted with rumen cannulas. For 13 months, the animals were maintained in 12 paddocks and fed a concentrate of ground corn, soybean meals, mineral supplements, and urea (URS) or encapsulated nitrate (EN) containing 70 g of EN/100 kg of BW (corresponding to 47 g NO3-/100 kg BW). Encapsulated nitrate supplementation resulted in similar forage, supplement and total DMI values as URS (P > 0.05), but ENS tended to increase (+48 g/d; P = 0.055) average daily weight gain. Daily reductions in methane emissions (-9.54 g or 18.5%) were observed with ENS when expressed as g of CH4/kg of forage dry matter intake (fDMI) (P = 0.037). Lower concentrations of NH3-N and a higher ruminal pH were observed in ENS groups 6 h after supplementation (P < 0.05). Total VFA rumen concentration 6 h (P = 0.009) and 12 h after supplementation with EN resulted in lower acetate concentrations in the rumen (P = 0.041). Steers supplemented with EN had a greater ruminal abundance of Bacteroides, Barnesiella, Lactobacillus, Selenomonas, Veillonella, Succinimonas, Succinivibrio, and Duganella sp. (P < 0.05), but a lower abundance of Methanobrevibacter sp. (P = 0.007). Strong negative correlations were found between daily methane emissions and Proteobacteria, Erysipelotrichaceae, Prevotellaceae, and Roseburia, Kandleria, Selenomonas, Veillonella, and Succinivibrio sp. (P < 0.05) in the rumen of ENS steers. Encapsulated nitrate is a feed additive that persistently affects enteric methane emission in grazing steers, thereby decreasing Methanobrevibacter abundance in the rumen. In addition, ENS can promote fumarate-reducer and lactate-producer bacteria, thereby reducing acetate production during rumen fermentation. |
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Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steersArchaea diversityBeef cattleEnteric methane emissionNitrateRumen bacteria diversityVolatile fatty acidsThis study investigated the long-term effects (13 months) of encapsulated nitrate supplementation (ENS) on enteric methane emissions, rumen fermentation parameters, ruminal bacteria, and diversity of archaea in grazing beef cattle. We used a total of thirty-two Nellore steers (initial BW of 197 ± 15.3 kg), 12 of which were fitted with rumen cannulas. For 13 months, the animals were maintained in 12 paddocks and fed a concentrate of ground corn, soybean meals, mineral supplements, and urea (URS) or encapsulated nitrate (EN) containing 70 g of EN/100 kg of BW (corresponding to 47 g NO3-/100 kg BW). Encapsulated nitrate supplementation resulted in similar forage, supplement and total DMI values as URS (P > 0.05), but ENS tended to increase (+48 g/d; P = 0.055) average daily weight gain. Daily reductions in methane emissions (-9.54 g or 18.5%) were observed with ENS when expressed as g of CH4/kg of forage dry matter intake (fDMI) (P = 0.037). Lower concentrations of NH3-N and a higher ruminal pH were observed in ENS groups 6 h after supplementation (P < 0.05). Total VFA rumen concentration 6 h (P = 0.009) and 12 h after supplementation with EN resulted in lower acetate concentrations in the rumen (P = 0.041). Steers supplemented with EN had a greater ruminal abundance of Bacteroides, Barnesiella, Lactobacillus, Selenomonas, Veillonella, Succinimonas, Succinivibrio, and Duganella sp. (P < 0.05), but a lower abundance of Methanobrevibacter sp. (P = 0.007). Strong negative correlations were found between daily methane emissions and Proteobacteria, Erysipelotrichaceae, Prevotellaceae, and Roseburia, Kandleria, Selenomonas, Veillonella, and Succinivibrio sp. (P < 0.05) in the rumen of ENS steers. Encapsulated nitrate is a feed additive that persistently affects enteric methane emission in grazing steers, thereby decreasing Methanobrevibacter abundance in the rumen. In addition, ENS can promote fumarate-reducer and lactate-producer bacteria, thereby reducing acetate production during rumen fermentation.Department of Animal Science Faculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual PaulistaDepartment of Animal Science Agência Paulista de Tecnologia Dos AgronegóciosGRASP Ind. and Com. LTDADepartment of Technology Faculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual PaulistaINCT/CA - UFV Department of Animal ScienceEmbrapa Southeast LivestockDepartment of Animal Science Faculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual PaulistaDepartment of Technology Faculdade de Ciências Agrárias e Veterinárias UNESP - Universidade Estadual PaulistaUniversidade Estadual Paulista (Unesp)Agência Paulista de Tecnologia Dos AgronegóciosGRASP Ind. and Com. LTDAUniversidade Federal de Viçosa (UFV)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Granja-Salcedo, Yury Tatiana [UNESP]Fernandes, Rodolfo MacIel [UNESP]De Araujo, Rafael CanonencoKishi, Luciano Takeshi [UNESP]Berchielli, Telma Teresinha [UNESP]De Resende, Flávio Dutra [UNESP]Berndt, AlexandreSiqueira, Gustavo Rezende [UNESP]2019-10-06T16:33:18Z2019-10-06T16:33:18Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fmicb.2019.00614Frontiers in Microbiology, v. 10, n. MAR, 2019.1664-302Xhttp://hdl.handle.net/11449/18920210.3389/fmicb.2019.006142-s2.0-85066628627Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T14:27:00Zoai:repositorio.unesp.br:11449/189202Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T14:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers |
title |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers |
spellingShingle |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers Granja-Salcedo, Yury Tatiana [UNESP] Archaea diversity Beef cattle Enteric methane emission Nitrate Rumen bacteria diversity Volatile fatty acids |
title_short |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers |
title_full |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers |
title_fullStr |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers |
title_full_unstemmed |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers |
title_sort |
Long-term encapsulated nitrate supplementation modulates rumen microbial diversity and rumen fermentation to reduce methane emission in grazing steers |
author |
Granja-Salcedo, Yury Tatiana [UNESP] |
author_facet |
Granja-Salcedo, Yury Tatiana [UNESP] Fernandes, Rodolfo MacIel [UNESP] De Araujo, Rafael Canonenco Kishi, Luciano Takeshi [UNESP] Berchielli, Telma Teresinha [UNESP] De Resende, Flávio Dutra [UNESP] Berndt, Alexandre Siqueira, Gustavo Rezende [UNESP] |
author_role |
author |
author2 |
Fernandes, Rodolfo MacIel [UNESP] De Araujo, Rafael Canonenco Kishi, Luciano Takeshi [UNESP] Berchielli, Telma Teresinha [UNESP] De Resende, Flávio Dutra [UNESP] Berndt, Alexandre Siqueira, Gustavo Rezende [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Agência Paulista de Tecnologia Dos Agronegócios GRASP Ind. and Com. LTDA Universidade Federal de Viçosa (UFV) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Granja-Salcedo, Yury Tatiana [UNESP] Fernandes, Rodolfo MacIel [UNESP] De Araujo, Rafael Canonenco Kishi, Luciano Takeshi [UNESP] Berchielli, Telma Teresinha [UNESP] De Resende, Flávio Dutra [UNESP] Berndt, Alexandre Siqueira, Gustavo Rezende [UNESP] |
dc.subject.por.fl_str_mv |
Archaea diversity Beef cattle Enteric methane emission Nitrate Rumen bacteria diversity Volatile fatty acids |
topic |
Archaea diversity Beef cattle Enteric methane emission Nitrate Rumen bacteria diversity Volatile fatty acids |
description |
This study investigated the long-term effects (13 months) of encapsulated nitrate supplementation (ENS) on enteric methane emissions, rumen fermentation parameters, ruminal bacteria, and diversity of archaea in grazing beef cattle. We used a total of thirty-two Nellore steers (initial BW of 197 ± 15.3 kg), 12 of which were fitted with rumen cannulas. For 13 months, the animals were maintained in 12 paddocks and fed a concentrate of ground corn, soybean meals, mineral supplements, and urea (URS) or encapsulated nitrate (EN) containing 70 g of EN/100 kg of BW (corresponding to 47 g NO3-/100 kg BW). Encapsulated nitrate supplementation resulted in similar forage, supplement and total DMI values as URS (P > 0.05), but ENS tended to increase (+48 g/d; P = 0.055) average daily weight gain. Daily reductions in methane emissions (-9.54 g or 18.5%) were observed with ENS when expressed as g of CH4/kg of forage dry matter intake (fDMI) (P = 0.037). Lower concentrations of NH3-N and a higher ruminal pH were observed in ENS groups 6 h after supplementation (P < 0.05). Total VFA rumen concentration 6 h (P = 0.009) and 12 h after supplementation with EN resulted in lower acetate concentrations in the rumen (P = 0.041). Steers supplemented with EN had a greater ruminal abundance of Bacteroides, Barnesiella, Lactobacillus, Selenomonas, Veillonella, Succinimonas, Succinivibrio, and Duganella sp. (P < 0.05), but a lower abundance of Methanobrevibacter sp. (P = 0.007). Strong negative correlations were found between daily methane emissions and Proteobacteria, Erysipelotrichaceae, Prevotellaceae, and Roseburia, Kandleria, Selenomonas, Veillonella, and Succinivibrio sp. (P < 0.05) in the rumen of ENS steers. Encapsulated nitrate is a feed additive that persistently affects enteric methane emission in grazing steers, thereby decreasing Methanobrevibacter abundance in the rumen. In addition, ENS can promote fumarate-reducer and lactate-producer bacteria, thereby reducing acetate production during rumen fermentation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:33:18Z 2019-10-06T16:33:18Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2019.00614 Frontiers in Microbiology, v. 10, n. MAR, 2019. 1664-302X http://hdl.handle.net/11449/189202 10.3389/fmicb.2019.00614 2-s2.0-85066628627 |
url |
http://dx.doi.org/10.3389/fmicb.2019.00614 http://hdl.handle.net/11449/189202 |
identifier_str_mv |
Frontiers in Microbiology, v. 10, n. MAR, 2019. 1664-302X 10.3389/fmicb.2019.00614 2-s2.0-85066628627 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964446235295744 |