Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.snb.2016.04.139 http://hdl.handle.net/11449/168604 |
Resumo: | Highly selective and sensitive modified electrodes have been the focus of many studies in sensing applications. Gene or enzymatic biosensors are greatly effective in this context, but they are often fragile and expensive. An alternative to these systems is the use of mimetic systems such as artificial enzyme immobilized in a matrix formed by thin films, for which the electrostatic layer-by-layer (LbL) technique has been widely applied. Here, the formation of bilayers or trilayers combining a cationic electrolyte (poly(allylamine) hydrochlorate, PAH), an anionic metallic complex (iron tetrasulfonated phthalocyanine, FeTsPc), an anionic phospholipid 1,2-dipalmitoyl-sn-3-glycero-(phosphor-rac-(1-glycerol), DPPG) and silver nanoparticles (AgNPs) arranged as (PAH/FeTsPc + DPPG)n and (PAH/FeTsPc + DPPG/AgNP)n was structurally and morphologically characterized. The electrostatic interaction of PAH, FeTsPc, and DPPG molecules were identified as the driven forces that allow the LbL film growth. The incorporation of AgNPs in the LbL films makes possible to active the surface enhanced resonant Raman scattering (SERRS) effect. The LbL films were evaluated as a sensor to catechol by cyclic voltammetry and impedance spectroscopy. The presence of AgNPs enhances the electrocatalytic activity of FeTsPc in the film to catechol oxidation. The (PAH/FeTsPc + DPPG/AgNP)5 LbL film presented a linear concentration range for catechol detection from 2 up to 100 μM, with a limit of detection of 0.87 μM. The distinction of catechol concentrations as well as discrimination among catechol, gallic and vanillic acid aqueous solutions was efficiently performed by principal component analysis (PCA) from impedance spectroscopy measurements. The LbL films here evaluated showed suitable properties to be applied as mimetic biosensors. The sensor was applied to polyphenol determination in green tea samples by standard addition showing high selectivity to catechol compared with results from Folin-Ciocalteu method. |
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Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detectionIron tetrasulfonated phthalocyanineLayer-by-layer filmsMimetic biosensorPhospholipidPolyphenol detectionSilver nanoparticlesHighly selective and sensitive modified electrodes have been the focus of many studies in sensing applications. Gene or enzymatic biosensors are greatly effective in this context, but they are often fragile and expensive. An alternative to these systems is the use of mimetic systems such as artificial enzyme immobilized in a matrix formed by thin films, for which the electrostatic layer-by-layer (LbL) technique has been widely applied. Here, the formation of bilayers or trilayers combining a cationic electrolyte (poly(allylamine) hydrochlorate, PAH), an anionic metallic complex (iron tetrasulfonated phthalocyanine, FeTsPc), an anionic phospholipid 1,2-dipalmitoyl-sn-3-glycero-(phosphor-rac-(1-glycerol), DPPG) and silver nanoparticles (AgNPs) arranged as (PAH/FeTsPc + DPPG)n and (PAH/FeTsPc + DPPG/AgNP)n was structurally and morphologically characterized. The electrostatic interaction of PAH, FeTsPc, and DPPG molecules were identified as the driven forces that allow the LbL film growth. The incorporation of AgNPs in the LbL films makes possible to active the surface enhanced resonant Raman scattering (SERRS) effect. The LbL films were evaluated as a sensor to catechol by cyclic voltammetry and impedance spectroscopy. The presence of AgNPs enhances the electrocatalytic activity of FeTsPc in the film to catechol oxidation. The (PAH/FeTsPc + DPPG/AgNP)5 LbL film presented a linear concentration range for catechol detection from 2 up to 100 μM, with a limit of detection of 0.87 μM. The distinction of catechol concentrations as well as discrimination among catechol, gallic and vanillic acid aqueous solutions was efficiently performed by principal component analysis (PCA) from impedance spectroscopy measurements. The LbL films here evaluated showed suitable properties to be applied as mimetic biosensors. The sensor was applied to polyphenol determination in green tea samples by standard addition showing high selectivity to catechol compared with results from Folin-Ciocalteu method.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federación Española de Enfermedades RarasMinisterio de Economía y CompetitividadFaculdade de Ciências e Tecnologia UNESP Univ. Estadual PaulistaDepartamento de Física de la Materia Condensada Facultad de Ciencias Universidad de ValladolidGrupo UVAsens. Escuela de Ingenierías Industriales Universidad de ValladolidFaculdade de Ciências e Tecnologia UNESP Univ. Estadual PaulistaFederación Española de Enfermedades Raras: CICYT-AGL2015-67482-RUniversidade Estadual Paulista (Unesp)Universidad de ValladolidAlessio, Priscila [UNESP]Martin, Cibely S. [UNESP]De Saja, Jose A.Rodriguez-Mendez, Maria L.2018-12-11T16:42:08Z2018-12-11T16:42:08Z2016-10-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article654-666application/pdfhttp://dx.doi.org/10.1016/j.snb.2016.04.139Sensors and Actuators, B: Chemical, v. 233, p. 654-666.0925-4005http://hdl.handle.net/11449/16860410.1016/j.snb.2016.04.1392-s2.0-849646241912-s2.0-84964624191.pdf97271222032192630000-0002-1345-0540Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSensors and Actuators, B: Chemical1,406info:eu-repo/semantics/openAccess2023-10-13T06:11:25Zoai:repositorio.unesp.br:11449/168604Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-13T06:11:25Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection |
title |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection |
spellingShingle |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection Alessio, Priscila [UNESP] Iron tetrasulfonated phthalocyanine Layer-by-layer films Mimetic biosensor Phospholipid Polyphenol detection Silver nanoparticles |
title_short |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection |
title_full |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection |
title_fullStr |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection |
title_full_unstemmed |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection |
title_sort |
Mimetic biosensors composed by layer-by-layer films of phospholipid, phthalocyanine and silver nanoparticles to polyphenol detection |
author |
Alessio, Priscila [UNESP] |
author_facet |
Alessio, Priscila [UNESP] Martin, Cibely S. [UNESP] De Saja, Jose A. Rodriguez-Mendez, Maria L. |
author_role |
author |
author2 |
Martin, Cibely S. [UNESP] De Saja, Jose A. Rodriguez-Mendez, Maria L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidad de Valladolid |
dc.contributor.author.fl_str_mv |
Alessio, Priscila [UNESP] Martin, Cibely S. [UNESP] De Saja, Jose A. Rodriguez-Mendez, Maria L. |
dc.subject.por.fl_str_mv |
Iron tetrasulfonated phthalocyanine Layer-by-layer films Mimetic biosensor Phospholipid Polyphenol detection Silver nanoparticles |
topic |
Iron tetrasulfonated phthalocyanine Layer-by-layer films Mimetic biosensor Phospholipid Polyphenol detection Silver nanoparticles |
description |
Highly selective and sensitive modified electrodes have been the focus of many studies in sensing applications. Gene or enzymatic biosensors are greatly effective in this context, but they are often fragile and expensive. An alternative to these systems is the use of mimetic systems such as artificial enzyme immobilized in a matrix formed by thin films, for which the electrostatic layer-by-layer (LbL) technique has been widely applied. Here, the formation of bilayers or trilayers combining a cationic electrolyte (poly(allylamine) hydrochlorate, PAH), an anionic metallic complex (iron tetrasulfonated phthalocyanine, FeTsPc), an anionic phospholipid 1,2-dipalmitoyl-sn-3-glycero-(phosphor-rac-(1-glycerol), DPPG) and silver nanoparticles (AgNPs) arranged as (PAH/FeTsPc + DPPG)n and (PAH/FeTsPc + DPPG/AgNP)n was structurally and morphologically characterized. The electrostatic interaction of PAH, FeTsPc, and DPPG molecules were identified as the driven forces that allow the LbL film growth. The incorporation of AgNPs in the LbL films makes possible to active the surface enhanced resonant Raman scattering (SERRS) effect. The LbL films were evaluated as a sensor to catechol by cyclic voltammetry and impedance spectroscopy. The presence of AgNPs enhances the electrocatalytic activity of FeTsPc in the film to catechol oxidation. The (PAH/FeTsPc + DPPG/AgNP)5 LbL film presented a linear concentration range for catechol detection from 2 up to 100 μM, with a limit of detection of 0.87 μM. The distinction of catechol concentrations as well as discrimination among catechol, gallic and vanillic acid aqueous solutions was efficiently performed by principal component analysis (PCA) from impedance spectroscopy measurements. The LbL films here evaluated showed suitable properties to be applied as mimetic biosensors. The sensor was applied to polyphenol determination in green tea samples by standard addition showing high selectivity to catechol compared with results from Folin-Ciocalteu method. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-05 2018-12-11T16:42:08Z 2018-12-11T16:42:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.snb.2016.04.139 Sensors and Actuators, B: Chemical, v. 233, p. 654-666. 0925-4005 http://hdl.handle.net/11449/168604 10.1016/j.snb.2016.04.139 2-s2.0-84964624191 2-s2.0-84964624191.pdf 9727122203219263 0000-0002-1345-0540 |
url |
http://dx.doi.org/10.1016/j.snb.2016.04.139 http://hdl.handle.net/11449/168604 |
identifier_str_mv |
Sensors and Actuators, B: Chemical, v. 233, p. 654-666. 0925-4005 10.1016/j.snb.2016.04.139 2-s2.0-84964624191 2-s2.0-84964624191.pdf 9727122203219263 0000-0002-1345-0540 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sensors and Actuators, B: Chemical 1,406 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
654-666 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964551577337856 |