Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s13071-015-1035-7 http://hdl.handle.net/11449/131381 |
Resumo: | The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America. A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition. The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae. Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae. |
id |
UNSP_c01d2272a68e6ce3f41aca439875af00 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/131381 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae)Sand fliesPhlebotomineImmature stagesLarval dietNyssomyia neivaiSubstratesSterilized dietThe most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America. A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition. The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae. Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Zoologia Animal, Universidade Estadual de Campinas, Campinas, SP 13083-970, Brazil.Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, UNESP - Univ Estadual Paulista, Araraquara, SP 14801-902, Brazil.Departamento de Ciências Biológicas, Faculdade de Ciências Farmacêuticas, UNESP - Univ Estadual Paulista, Araraquara, SP, 14801-902, Brazil.FAPESP: 2012/23832–9BioMed CentralUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Goulart, Thais MarchiCastro, Camila Feitosa de [UNESP]Machado, Vicente Estevam [UNESP]Silva, Flávia Benini da Rocha [UNESP]Pinto, Mara Cristina [UNESP]2015-12-07T15:34:35Z2015-12-07T15:34:35Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://dx.doi.org/10.1186/s13071-015-1035-7Parasites & Vectors, v. 8, p. 1-7, 2015.1756-3305http://hdl.handle.net/11449/13138110.1186/s13071-015-1035-7PMC4537559.pdf822173749159841526276040PMC4537559PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasites & Vectors3.1631,702info:eu-repo/semantics/openAccess2023-12-31T06:21:52Zoai:repositorio.unesp.br:11449/131381Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-31T06:21:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) |
title |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) |
spellingShingle |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) Goulart, Thais Marchi Sand flies Phlebotomine Immature stages Larval diet Nyssomyia neivai Substrates Sterilized diet |
title_short |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) |
title_full |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) |
title_fullStr |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) |
title_full_unstemmed |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) |
title_sort |
Techniques to improve the maintenance of a laboratory colony of Nyssomyia neivai (Diptera: Psychodidae) |
author |
Goulart, Thais Marchi |
author_facet |
Goulart, Thais Marchi Castro, Camila Feitosa de [UNESP] Machado, Vicente Estevam [UNESP] Silva, Flávia Benini da Rocha [UNESP] Pinto, Mara Cristina [UNESP] |
author_role |
author |
author2 |
Castro, Camila Feitosa de [UNESP] Machado, Vicente Estevam [UNESP] Silva, Flávia Benini da Rocha [UNESP] Pinto, Mara Cristina [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Goulart, Thais Marchi Castro, Camila Feitosa de [UNESP] Machado, Vicente Estevam [UNESP] Silva, Flávia Benini da Rocha [UNESP] Pinto, Mara Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Sand flies Phlebotomine Immature stages Larval diet Nyssomyia neivai Substrates Sterilized diet |
topic |
Sand flies Phlebotomine Immature stages Larval diet Nyssomyia neivai Substrates Sterilized diet |
description |
The most critical phase in sand fly colonization is the high mortality in the larval instars. In this study, we sought out strategies for improving the colonization of Nyssomyia neivai, one of the vectors of cutaneous leishmaniasis agent in South America. A colony of Ny. neivai was established in the laboratory from a field population, and the productivity of adults was evaluated considering carrying capacity, diet for larvae and surface for oviposition. The highest emergency rate of adults was achieved with the fewest couples inside 150 mL rearing chambers on a sterilized diet made of rabbit feces, rabbit food, soil and fish food and with vermiculite as a substrate for oviposition and the development of larvae. Our data on Ny. neivai colonization showed that the best adult productivities were achieved with fewer couples inside the rearing chambers; smaller rearing containers of 150 mL (due to less fungi growth); sterilized diet made of rabbit feces, rabbit food, soil and fish food; and vermiculite as the substrate for oviposition and development of larvae. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:34:35Z 2015-12-07T15:34:35Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s13071-015-1035-7 Parasites & Vectors, v. 8, p. 1-7, 2015. 1756-3305 http://hdl.handle.net/11449/131381 10.1186/s13071-015-1035-7 PMC4537559.pdf 8221737491598415 26276040 PMC4537559 |
url |
http://dx.doi.org/10.1186/s13071-015-1035-7 http://hdl.handle.net/11449/131381 |
identifier_str_mv |
Parasites & Vectors, v. 8, p. 1-7, 2015. 1756-3305 10.1186/s13071-015-1035-7 PMC4537559.pdf 8221737491598415 26276040 PMC4537559 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasites & Vectors 3.163 1,702 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 application/pdf |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792962223727968256 |