Níveis de fósforo em dietas para Zebrafish Danio rerio

• Autor(a) principal: Costa, Juliana Mara [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/136058; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/19-02-2016/000858926.pdf
• topic: Peixe ornamental - Alimentação e rações; Fosforo na nutrição animal; Ossos - Anomalias; Expressão gênica; Fishes

The growth assay was performed to estimate the dicalcium phosphate 4 phosphorus (P) availability, the phosphorus requirement and external deficiency signs 5 in diet of zebrafish (Danio rerio) allocated in 15 L aquarium . Six semipurified diets 6 were used with graded total phosphorus levels (0.35, 0.65, 0.95, 1.25, 1.55 and 1.85%) 7 for 8 weeks. The basal diet (T1), with 0.35% total phosphorus was not supplemented 8 with phosphorous mineral source, and all diets were formulated with inorganic 9 phosphate inclusion increasing. For the experiment 450 zebrafish fingerlings were used 10 (0.0079 ± 0,0003g average weight with 31 days post-hatching), distributed in a 11 completely randomized design experiment with five replications. The fish were fed six 12 times a day at 28 ± 1 ᵒC, 6.8 ± 0, 32 pH, 0.06 ± 0.01 mg L-1 ammonia concentration 13 and 6.5 ± 0.3 mg L-1dissolved oxygen. Dicalcium phosphate phosphorus availability 14 was 53.84%. Feed Conversion (FC), Specific Growth Rate (SGR), Growth (G) and the 15 final Length (L) had a positive correlation with phosphorus diet levels (p> 0.01). The 16 phosphorus retention efficiency was higher in fish fed 0.35 and 0.65% phosphorus diets. 17 The fish body composition analyzes showed that phosphorus, calcium, magnesium, dry 18 matter and lipids were significantly affected by phosphorus diets concentration (p> 19 0.01), however, not significant for body manganese, zinc and copper concentration 20 among treatments. Phosphorus deficiency signs were characterized by lower growth, 21 low appetite, increased body lipid contents, external appearance of skeletal deformity 22 (cranial, lordosis and scoliosis), mineralization bone reduction (bone tissue thickness). It 23 can be concluded that the better phosphorus level was 1.55% for all parameters 24 evaluated. 25 26 ...