A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2527/1996.7481915x http://hdl.handle.net/11449/219194 |
Resumo: | To determine whether injection of hCG or GnRH-agonist on d 5 after estrus (d 0) has a differential functional effect on an induced and the original corpus luteum (CL), two experiments were conducted. In Exp. 1, nonlactating Holstein cows were injected on d 5 with saline (n = 4; T1), a GnRH-agonist (Buserelin®, 8 μg i.m.; n = 4; T2), or hCG (1,000 IU, i.v., and 2,000 IU, i.m.; n = 4; T3). Induced CL were removed on d 13 and weights were different (GnRH-agonist < hCG). In vitro production of progesterone by CL tissue (μg/g; μg/CL) was affected by treatment (GnRH-agonist < hCG) and dose of LH (ng/mL) in culture media. Experiment 2 was a replicate of Exp. 1, except that the original CL was removed on d 17 for in vitro culture. Day-17 CL weights and in vitro production of progesterone by original CL were not affected by treatment. The daily rate of increase of plasma progesterone from d 6 to d 13 differed: saline < GnRH-agonist < hCG (P < .01). From d 14 to 17, the rate of plasma progesterone decrease was not different between treatments. Electron micrographic study of the original and induced CL indicates that LH-like exposure delays involution of steroidogenic luteal cells. In summary, the higher levels of progesterone from d 6 to d 13 of the estrous cycle following an injection of hCG vs GnRH-agonist on d 5 is due to a greater response of hCG-induced CL. |
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A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous CycleCorpus LuteumGonadotropinshCGLuteal CellsProgesteroneTo determine whether injection of hCG or GnRH-agonist on d 5 after estrus (d 0) has a differential functional effect on an induced and the original corpus luteum (CL), two experiments were conducted. In Exp. 1, nonlactating Holstein cows were injected on d 5 with saline (n = 4; T1), a GnRH-agonist (Buserelin®, 8 μg i.m.; n = 4; T2), or hCG (1,000 IU, i.v., and 2,000 IU, i.m.; n = 4; T3). Induced CL were removed on d 13 and weights were different (GnRH-agonist < hCG). In vitro production of progesterone by CL tissue (μg/g; μg/CL) was affected by treatment (GnRH-agonist < hCG) and dose of LH (ng/mL) in culture media. Experiment 2 was a replicate of Exp. 1, except that the original CL was removed on d 17 for in vitro culture. Day-17 CL weights and in vitro production of progesterone by original CL were not affected by treatment. The daily rate of increase of plasma progesterone from d 6 to d 13 differed: saline < GnRH-agonist < hCG (P < .01). From d 14 to 17, the rate of plasma progesterone decrease was not different between treatments. Electron micrographic study of the original and induced CL indicates that LH-like exposure delays involution of steroidogenic luteal cells. In summary, the higher levels of progesterone from d 6 to d 13 of the estrous cycle following an injection of hCG vs GnRH-agonist on d 5 is due to a greater response of hCG-induced CL.Dept. of Dairy and Poultry Sciences University of Florida, Gainesville, FL 32611Department of Animal Science University of Florida, Gainesville, FL 32611Department of Pharmacology Univ. Estadual de São Paulo, São Paulo 18600Dept. of Struct. and Cell. Biology University of South Alabama, Mobile, AL 36688University of FloridaUniv. Estadual de São PauloUniversity of South AlabamaSchmitt, É J.P.Barros, C. M.Fields, P. A.Fields, M. J.Diaz, T.Kluge, J. M.Thatcher, W. W.2022-04-28T18:54:10Z2022-04-28T18:54:10Z1996-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1915-1929http://dx.doi.org/10.2527/1996.7481915xJournal of Animal Science, v. 74, n. 8, p. 1915-1929, 1996.0021-8812http://hdl.handle.net/11449/21919410.2527/1996.7481915x2-s2.0-0030207326Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Animal Scienceinfo:eu-repo/semantics/openAccess2022-04-28T18:54:10Zoai:repositorio.unesp.br:11449/219194Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T18:54:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle |
title |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle |
spellingShingle |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle Schmitt, É J.P. Corpus Luteum Gonadotropins hCG Luteal Cells Progesterone |
title_short |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle |
title_full |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle |
title_fullStr |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle |
title_full_unstemmed |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle |
title_sort |
A Cellular and Endocrine Characterization of the Original and Induced Corpus Luteum after Administration of a Gonadotropin-Releasing Hormone Agonist or Human Chorionic Gonadotropin on Day Five of the Estrous Cycle |
author |
Schmitt, É J.P. |
author_facet |
Schmitt, É J.P. Barros, C. M. Fields, P. A. Fields, M. J. Diaz, T. Kluge, J. M. Thatcher, W. W. |
author_role |
author |
author2 |
Barros, C. M. Fields, P. A. Fields, M. J. Diaz, T. Kluge, J. M. Thatcher, W. W. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
University of Florida Univ. Estadual de São Paulo University of South Alabama |
dc.contributor.author.fl_str_mv |
Schmitt, É J.P. Barros, C. M. Fields, P. A. Fields, M. J. Diaz, T. Kluge, J. M. Thatcher, W. W. |
dc.subject.por.fl_str_mv |
Corpus Luteum Gonadotropins hCG Luteal Cells Progesterone |
topic |
Corpus Luteum Gonadotropins hCG Luteal Cells Progesterone |
description |
To determine whether injection of hCG or GnRH-agonist on d 5 after estrus (d 0) has a differential functional effect on an induced and the original corpus luteum (CL), two experiments were conducted. In Exp. 1, nonlactating Holstein cows were injected on d 5 with saline (n = 4; T1), a GnRH-agonist (Buserelin®, 8 μg i.m.; n = 4; T2), or hCG (1,000 IU, i.v., and 2,000 IU, i.m.; n = 4; T3). Induced CL were removed on d 13 and weights were different (GnRH-agonist < hCG). In vitro production of progesterone by CL tissue (μg/g; μg/CL) was affected by treatment (GnRH-agonist < hCG) and dose of LH (ng/mL) in culture media. Experiment 2 was a replicate of Exp. 1, except that the original CL was removed on d 17 for in vitro culture. Day-17 CL weights and in vitro production of progesterone by original CL were not affected by treatment. The daily rate of increase of plasma progesterone from d 6 to d 13 differed: saline < GnRH-agonist < hCG (P < .01). From d 14 to 17, the rate of plasma progesterone decrease was not different between treatments. Electron micrographic study of the original and induced CL indicates that LH-like exposure delays involution of steroidogenic luteal cells. In summary, the higher levels of progesterone from d 6 to d 13 of the estrous cycle following an injection of hCG vs GnRH-agonist on d 5 is due to a greater response of hCG-induced CL. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996-01-01 2022-04-28T18:54:10Z 2022-04-28T18:54:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2527/1996.7481915x Journal of Animal Science, v. 74, n. 8, p. 1915-1929, 1996. 0021-8812 http://hdl.handle.net/11449/219194 10.2527/1996.7481915x 2-s2.0-0030207326 |
url |
http://dx.doi.org/10.2527/1996.7481915x http://hdl.handle.net/11449/219194 |
identifier_str_mv |
Journal of Animal Science, v. 74, n. 8, p. 1915-1929, 1996. 0021-8812 10.2527/1996.7481915x 2-s2.0-0030207326 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Animal Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1915-1929 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1799965570436694016 |