Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.oooo.2020.06.003 http://hdl.handle.net/11449/201953 |
Resumo: | Objective: The aim of this study was to evaluate the expression of DNA repair genes in cases of oral squamous cell carcinoma (OSCC). Study Design: Expression of the MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes was evaluated by reverse transcription–quantitative polymerase chain reaction in the OSCC group (32 patients) and the control group (15 patients). The groups were compared by using the Mann-Whitney test, with Bonferroni correction. Associations between gene expression levels and clinical data were explored by using Pearson's and Spearman's correlation coefficients, with P value less than. 05 indicating a significant difference. Results: The MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes were downregulated in the OSCC group compared with the control group, with significant values for MLH1 (P < .0001); MSH2 (P = .038); MLH3 (P < .0001); ATM (P < .0001); MRE11A (P < .0001); XRCC1 (P = .0004); and PMS2 (P = .008). Analysis of the correlation between gene expression and clinical data only revealed a significant negative correlation between age and expression of the PMS2 gene. Conclusions: Expression of the DNA repair genes MLH1, MSH2, MLH3, ATM, MRE11 AMRE11A, XRCC1, and PMS2 was reduced in OSCC. |
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Repositório Institucional da UNESP |
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Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reactionObjective: The aim of this study was to evaluate the expression of DNA repair genes in cases of oral squamous cell carcinoma (OSCC). Study Design: Expression of the MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes was evaluated by reverse transcription–quantitative polymerase chain reaction in the OSCC group (32 patients) and the control group (15 patients). The groups were compared by using the Mann-Whitney test, with Bonferroni correction. Associations between gene expression levels and clinical data were explored by using Pearson's and Spearman's correlation coefficients, with P value less than. 05 indicating a significant difference. Results: The MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes were downregulated in the OSCC group compared with the control group, with significant values for MLH1 (P < .0001); MSH2 (P = .038); MLH3 (P < .0001); ATM (P < .0001); MRE11A (P < .0001); XRCC1 (P = .0004); and PMS2 (P = .008). Analysis of the correlation between gene expression and clinical data only revealed a significant negative correlation between age and expression of the PMS2 gene. Conclusions: Expression of the DNA repair genes MLH1, MSH2, MLH3, ATM, MRE11 AMRE11A, XRCC1, and PMS2 was reduced in OSCC.School of Dentistry Universidade Mogi das CruzesSchool of Dentistry Brazcubas EducaçãoDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Department of Head and Neck Surgery São Leopoldo Mandic Medical SchoolDepartment of Head and Neck Surgery Hospital Municipal Dr. Mário GattiDepartment of Oral Pathology School of Dentistry University of São PauloSchool of Medicine Anhembi Morumbi University São José dos CamposDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (UNESP)Universidade Mogi das CruzesBrazcubas EducaçãoUniversidade Estadual Paulista (Unesp)São Leopoldo Mandic Medical SchoolHospital Municipal Dr. Mário GattiUniversidade de São Paulo (USP)São José dos CamposOliveira Alves, Mônica Ghislaineda Silva Miguel, NatáliaFerreira, Camila Cristina Panisello [UNESP]Alvarenga, Elis RibeiroTonon, Bruna Manzanaresde Barros, Patrícia Pimentel [UNESP]Carta, Celina Faig Lima [UNESP]Paschoal, Maria Beatriz Nogueirade Sales Chagas, José FranciscoBandeira, Celso Muller [UNESP]Nunes, Fábio DaumasAlmeida, Janete Dias [UNESP]2020-12-12T02:46:03Z2020-12-12T02:46:03Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.oooo.2020.06.003Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology.2212-4403http://hdl.handle.net/11449/20195310.1016/j.oooo.2020.06.0032-s2.0-85087938403Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOral Surgery, Oral Medicine, Oral Pathology and Oral Radiologyinfo:eu-repo/semantics/openAccess2021-10-23T03:21:30Zoai:repositorio.unesp.br:11449/201953Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T03:21:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction |
title |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction |
spellingShingle |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction Oliveira Alves, Mônica Ghislaine |
title_short |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction |
title_full |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction |
title_fullStr |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction |
title_full_unstemmed |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction |
title_sort |
Expression of DNA repair genes in oral squamous cell carcinoma using reverse transcription-quantitative polymerase chain reaction |
author |
Oliveira Alves, Mônica Ghislaine |
author_facet |
Oliveira Alves, Mônica Ghislaine da Silva Miguel, Natália Ferreira, Camila Cristina Panisello [UNESP] Alvarenga, Elis Ribeiro Tonon, Bruna Manzanares de Barros, Patrícia Pimentel [UNESP] Carta, Celina Faig Lima [UNESP] Paschoal, Maria Beatriz Nogueira de Sales Chagas, José Francisco Bandeira, Celso Muller [UNESP] Nunes, Fábio Daumas Almeida, Janete Dias [UNESP] |
author_role |
author |
author2 |
da Silva Miguel, Natália Ferreira, Camila Cristina Panisello [UNESP] Alvarenga, Elis Ribeiro Tonon, Bruna Manzanares de Barros, Patrícia Pimentel [UNESP] Carta, Celina Faig Lima [UNESP] Paschoal, Maria Beatriz Nogueira de Sales Chagas, José Francisco Bandeira, Celso Muller [UNESP] Nunes, Fábio Daumas Almeida, Janete Dias [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Mogi das Cruzes Brazcubas Educação Universidade Estadual Paulista (Unesp) São Leopoldo Mandic Medical School Hospital Municipal Dr. Mário Gatti Universidade de São Paulo (USP) São José dos Campos |
dc.contributor.author.fl_str_mv |
Oliveira Alves, Mônica Ghislaine da Silva Miguel, Natália Ferreira, Camila Cristina Panisello [UNESP] Alvarenga, Elis Ribeiro Tonon, Bruna Manzanares de Barros, Patrícia Pimentel [UNESP] Carta, Celina Faig Lima [UNESP] Paschoal, Maria Beatriz Nogueira de Sales Chagas, José Francisco Bandeira, Celso Muller [UNESP] Nunes, Fábio Daumas Almeida, Janete Dias [UNESP] |
description |
Objective: The aim of this study was to evaluate the expression of DNA repair genes in cases of oral squamous cell carcinoma (OSCC). Study Design: Expression of the MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes was evaluated by reverse transcription–quantitative polymerase chain reaction in the OSCC group (32 patients) and the control group (15 patients). The groups were compared by using the Mann-Whitney test, with Bonferroni correction. Associations between gene expression levels and clinical data were explored by using Pearson's and Spearman's correlation coefficients, with P value less than. 05 indicating a significant difference. Results: The MLH1, MSH2, MLH3, ATM, MRE11A, XRCC1, and PMS2 genes were downregulated in the OSCC group compared with the control group, with significant values for MLH1 (P < .0001); MSH2 (P = .038); MLH3 (P < .0001); ATM (P < .0001); MRE11A (P < .0001); XRCC1 (P = .0004); and PMS2 (P = .008). Analysis of the correlation between gene expression and clinical data only revealed a significant negative correlation between age and expression of the PMS2 gene. Conclusions: Expression of the DNA repair genes MLH1, MSH2, MLH3, ATM, MRE11 AMRE11A, XRCC1, and PMS2 was reduced in OSCC. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:46:03Z 2020-12-12T02:46:03Z 2020-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.oooo.2020.06.003 Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 2212-4403 http://hdl.handle.net/11449/201953 10.1016/j.oooo.2020.06.003 2-s2.0-85087938403 |
url |
http://dx.doi.org/10.1016/j.oooo.2020.06.003 http://hdl.handle.net/11449/201953 |
identifier_str_mv |
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 2212-4403 10.1016/j.oooo.2020.06.003 2-s2.0-85087938403 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790047423954944 |