Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/121862 |
Resumo: | The cardiotoxicity induced by doxorubicin generates myocardial remodeling and systolic dysfunction. The present study evaluated the role of apoptosis and extracellular matrix components (fibronectin and myofibroblasts) in doxorubicin-induced dilated cardiomyopathy in rabbits. Twenty five New Zealand rabbits were used, allocated into two groups (control and treated). The drug was administered for six weeks and Doppler echocardiography was performed before the first and after the last administration. Myocardial remodeling was evaluated by electron microscopy (scanning and transmission) and immunodetection of apoptotic cells, myofibroblasts and fibronectin. Significant reduction in systolic function, increased apoptotic fibers and myofibroblasts were noticed in treated animals, on the left ventricle, interventricular septum and right ventricle. There was a significant negative correlation between the number of damaged mitochondria and apoptotic cells at the left ventricle and interventricular septum with systolic function, showing that the apoptosis by mitochondrial pathway plays a role on the systolic dysfunction during treatment with doxorubicin and the increase in extracellular matrix is not the primary cause of systolic dysfunction induced by doxorubicin |
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Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhosCoelhoMiocardio - DoençasDoppler, EcocardiografiaImuno-histoquímicaMicroscopia eletronicaApoptoseImmunohistochemistryThe cardiotoxicity induced by doxorubicin generates myocardial remodeling and systolic dysfunction. The present study evaluated the role of apoptosis and extracellular matrix components (fibronectin and myofibroblasts) in doxorubicin-induced dilated cardiomyopathy in rabbits. Twenty five New Zealand rabbits were used, allocated into two groups (control and treated). The drug was administered for six weeks and Doppler echocardiography was performed before the first and after the last administration. Myocardial remodeling was evaluated by electron microscopy (scanning and transmission) and immunodetection of apoptotic cells, myofibroblasts and fibronectin. Significant reduction in systolic function, increased apoptotic fibers and myofibroblasts were noticed in treated animals, on the left ventricle, interventricular septum and right ventricle. There was a significant negative correlation between the number of damaged mitochondria and apoptotic cells at the left ventricle and interventricular septum with systolic function, showing that the apoptosis by mitochondrial pathway plays a role on the systolic dysfunction during treatment with doxorubicin and the increase in extracellular matrix is not the primary cause of systolic dysfunction induced by doxorubicinA cardiotoxicidade induzida pela doxorrubicina gera remodelamento miocárdico e disfunção sistólica. O presente estudo avaliou a participação da apoptose e componentes da matriz extracelular (miofibroblastos e fibronectina) na cardiomiopatia dilatada induzida com doxorrubicina em coelhos. Foram utilizados 25 coelhos da raça Nova Zelândia, alocados em dois grupos (controle e tratados). O fármaco foi administrado por seis semanas e a ecodopplercardiografia foi realizada no momento zero e após a última administração. O remodelamento do miocárdio foi avaliado por microscopia eletrônica (varredura e transmissão) e por imunodetecção de fibras apoptóticas, miofibroblastos e fibronectina. Nos animais tratados houve redução significativa da função sistólica observada na ecodopplercardiografia e aumento das fibras apoptóticas e miofibroblastos, no ventrículo esquerdo, septo interventricular e ventrículo direito. Houve correlação negativa significativa entre o número de mitocôndrias lesadas e das células apoptóticas no septo interventricular e no ventrículo esquerdo com os índices de função sistólica. Os dados obtidos permitem inferir que a apoptose por via mitocondrial participa da disfunção sistólica observada no tratamento com doxorrubicina e que o aumento da matriz extracelular não é o principal causador de disfunção sistólica na cardiomiopatia dilatada induzida com doxorrubicinaUniversidade Estadual Paulista (Unesp)Camacho, Aparecido Antonio [UNESP]Universidade Estadual Paulista (Unesp)Gava, Fábio Nelson [UNESP]2015-04-09T12:28:07Z2015-04-09T12:28:07Z2014-02-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisxvi, 56 p. : il.application/pdfGAVA, Fábio Nelson. Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos. 2014. xvi, 56 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014.http://hdl.handle.net/11449/121862000814364000814364.pdf33004102072P99642688764978907Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-11-19T06:07:38Zoai:repositorio.unesp.br:11449/121862Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-19T06:07:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos |
title |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos |
spellingShingle |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos Gava, Fábio Nelson [UNESP] Coelho Miocardio - Doenças Doppler, Ecocardiografia Imuno-histoquímica Microscopia eletronica Apoptose Immunohistochemistry |
title_short |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos |
title_full |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos |
title_fullStr |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos |
title_full_unstemmed |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos |
title_sort |
Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos |
author |
Gava, Fábio Nelson [UNESP] |
author_facet |
Gava, Fábio Nelson [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Camacho, Aparecido Antonio [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Gava, Fábio Nelson [UNESP] |
dc.subject.por.fl_str_mv |
Coelho Miocardio - Doenças Doppler, Ecocardiografia Imuno-histoquímica Microscopia eletronica Apoptose Immunohistochemistry |
topic |
Coelho Miocardio - Doenças Doppler, Ecocardiografia Imuno-histoquímica Microscopia eletronica Apoptose Immunohistochemistry |
description |
The cardiotoxicity induced by doxorubicin generates myocardial remodeling and systolic dysfunction. The present study evaluated the role of apoptosis and extracellular matrix components (fibronectin and myofibroblasts) in doxorubicin-induced dilated cardiomyopathy in rabbits. Twenty five New Zealand rabbits were used, allocated into two groups (control and treated). The drug was administered for six weeks and Doppler echocardiography was performed before the first and after the last administration. Myocardial remodeling was evaluated by electron microscopy (scanning and transmission) and immunodetection of apoptotic cells, myofibroblasts and fibronectin. Significant reduction in systolic function, increased apoptotic fibers and myofibroblasts were noticed in treated animals, on the left ventricle, interventricular septum and right ventricle. There was a significant negative correlation between the number of damaged mitochondria and apoptotic cells at the left ventricle and interventricular septum with systolic function, showing that the apoptosis by mitochondrial pathway plays a role on the systolic dysfunction during treatment with doxorubicin and the increase in extracellular matrix is not the primary cause of systolic dysfunction induced by doxorubicin |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-18 2015-04-09T12:28:07Z 2015-04-09T12:28:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
GAVA, Fábio Nelson. Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos. 2014. xvi, 56 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014. http://hdl.handle.net/11449/121862 000814364 000814364.pdf 33004102072P9 9642688764978907 |
identifier_str_mv |
GAVA, Fábio Nelson. Remodelamento do miocárdio na cardiomiopatia dilatada induzida com doxorrubicina em coelhos. 2014. xvi, 56 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2014. 000814364 000814364.pdf 33004102072P9 9642688764978907 |
url |
http://hdl.handle.net/11449/121862 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
xvi, 56 p. : il. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961860080762880 |