Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s40729-020-00261-3 http://hdl.handle.net/11449/209590 |
Resumo: | Background An effective biomaterial for bone replacement should have properties to avoid bacterial contamination and promote bone formation while inducing rapid cell differentiation simultaneously. Bone marrow stem cells are currently being investigated because of their known potential for differentiation in osteoblast lineage. This makes these cells a good option for stem cell-based therapy. We have aimed to analyze, in vitro, the potential of pure titanium (Ti), Ti-35Nb-7Zr alloy (A), niobium (Nb), and zirconia (Zr) to avoid the microorganisms S. aureus (S.a) and P. aeruginosa (P.a). Furthermore, our objective was to evaluate if the basic elements of Ti-35Nb-7Zr alloy have any influence on bone marrow stromal cells, the source of stem cells, and observe if these metals have properties to induce cell differentiation into osteoblasts. Methods Bone marrow stromal cells (BMSC) were obtained from mice femurs and cultured in osteogenic media without dexamethasone as an external source of cell differentiation. The samples were divided into Ti-35Nb-7Zr alloy (A), pure titanium (Ti), Nb (niobium), and Zr (zirconia) and were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). After predetermined periods, cell interaction, cytotoxicity, proliferation, and cell differentiation tests were performed. For monotypic biofilm formation, standardized suspensions (10(6) cells/ml) with the microorganisms S. aureus (S.a) and P. aeruginosa (P.a) were cultured for 24 h on the samples and submitted to an MTT test. Results All samples presented cell proliferation, growth, and spreading. All groups presented cell viability above 70%, but the alloy (A) showed better results, with statistical differences from Nb and Zr samples. Zr expressed higher ALP activity and was statistically different from the other groups (p < 0.05). In contrast, no statistical difference was observed between the samples as regards mineralization nodules. Lower biofilm formation of S.a and P.a. was observed on the Nb samples, with statistical differences from the other samples. Conclusion Our results suggest that the basic elements present in the alloy have osteoinductive characteristics, and Zr has a good influence on bone marrow stromal cell differentiation. We also believe that Nb has the best potential for reducing the formation of microbial biofilms. |
id |
UNSP_c202cae62200e82a7934326c27c2aa1e |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/209590 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineeringTi-35Nb-7Zr alloyBiofilmBone marrow cellsOsteogenesisBackground An effective biomaterial for bone replacement should have properties to avoid bacterial contamination and promote bone formation while inducing rapid cell differentiation simultaneously. Bone marrow stem cells are currently being investigated because of their known potential for differentiation in osteoblast lineage. This makes these cells a good option for stem cell-based therapy. We have aimed to analyze, in vitro, the potential of pure titanium (Ti), Ti-35Nb-7Zr alloy (A), niobium (Nb), and zirconia (Zr) to avoid the microorganisms S. aureus (S.a) and P. aeruginosa (P.a). Furthermore, our objective was to evaluate if the basic elements of Ti-35Nb-7Zr alloy have any influence on bone marrow stromal cells, the source of stem cells, and observe if these metals have properties to induce cell differentiation into osteoblasts. Methods Bone marrow stromal cells (BMSC) were obtained from mice femurs and cultured in osteogenic media without dexamethasone as an external source of cell differentiation. The samples were divided into Ti-35Nb-7Zr alloy (A), pure titanium (Ti), Nb (niobium), and Zr (zirconia) and were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). After predetermined periods, cell interaction, cytotoxicity, proliferation, and cell differentiation tests were performed. For monotypic biofilm formation, standardized suspensions (10(6) cells/ml) with the microorganisms S. aureus (S.a) and P. aeruginosa (P.a) were cultured for 24 h on the samples and submitted to an MTT test. Results All samples presented cell proliferation, growth, and spreading. All groups presented cell viability above 70%, but the alloy (A) showed better results, with statistical differences from Nb and Zr samples. Zr expressed higher ALP activity and was statistically different from the other groups (p < 0.05). In contrast, no statistical difference was observed between the samples as regards mineralization nodules. Lower biofilm formation of S.a and P.a. was observed on the Nb samples, with statistical differences from the other samples. Conclusion Our results suggest that the basic elements present in the alloy have osteoinductive characteristics, and Zr has a good influence on bone marrow stromal cell differentiation. We also believe that Nb has the best potential for reducing the formation of microbial biofilms.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Estadual Paulista UNESP, Sao Jose Dos Campos Sch Dent, Dept Biosci & Oral Diag, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, BrazilOakland Univ Math & Sci, 318 Meadow Brook Rd, Rochester Hills, MI 48309 USAEscola Engn Lorena EEL USP, Gleba Al-6 S-N, Lorena, SP, BrazilUniv Estadual Paulista UNESP, Sao Jose Dos Campos Sch Dent, Dept Biosci & Oral Diag, Av Engenheiro Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP, BrazilSpringerUniversidade Estadual Paulista (Unesp)Oakland Univ Math & SciUniversidade de São Paulo (USP)Camargo Reis Mello, Daphne de [UNESP]Rodrigues, Lais Morandini [UNESP]D'Antola Mello, Fabia Zampieri [UNESP]Goncalves, Thais Fernanda [UNESP]Ferreira, BentoSchneider, Sandra GiacominOliveira, Luciane Dias de [UNESP]Vasconcellos, Luana Marotta Reis de [UNESP]2021-06-25T12:23:19Z2021-06-25T12:23:19Z2020-10-25info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.1186/s40729-020-00261-3International Journal Of Implant Dentistry. Tokyo: Springer Japan Kk, v. 6, n. 1, 11 p., 2020.http://hdl.handle.net/11449/20959010.1186/s40729-020-00261-3WOS:000582333100001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Implant Dentistryinfo:eu-repo/semantics/openAccess2021-10-23T19:28:17Zoai:repositorio.unesp.br:11449/209590Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:28:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering |
title |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering |
spellingShingle |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering Camargo Reis Mello, Daphne de [UNESP] Ti-35Nb-7Zr alloy Biofilm Bone marrow cells Osteogenesis |
title_short |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering |
title_full |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering |
title_fullStr |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering |
title_full_unstemmed |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering |
title_sort |
Biological and microbiological interactions of Ti-35Nb-7Zr alloy and its basic elements on bone marrow stromal cells: good prospects for bone tissue engineering |
author |
Camargo Reis Mello, Daphne de [UNESP] |
author_facet |
Camargo Reis Mello, Daphne de [UNESP] Rodrigues, Lais Morandini [UNESP] D'Antola Mello, Fabia Zampieri [UNESP] Goncalves, Thais Fernanda [UNESP] Ferreira, Bento Schneider, Sandra Giacomin Oliveira, Luciane Dias de [UNESP] Vasconcellos, Luana Marotta Reis de [UNESP] |
author_role |
author |
author2 |
Rodrigues, Lais Morandini [UNESP] D'Antola Mello, Fabia Zampieri [UNESP] Goncalves, Thais Fernanda [UNESP] Ferreira, Bento Schneider, Sandra Giacomin Oliveira, Luciane Dias de [UNESP] Vasconcellos, Luana Marotta Reis de [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Oakland Univ Math & Sci Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Camargo Reis Mello, Daphne de [UNESP] Rodrigues, Lais Morandini [UNESP] D'Antola Mello, Fabia Zampieri [UNESP] Goncalves, Thais Fernanda [UNESP] Ferreira, Bento Schneider, Sandra Giacomin Oliveira, Luciane Dias de [UNESP] Vasconcellos, Luana Marotta Reis de [UNESP] |
dc.subject.por.fl_str_mv |
Ti-35Nb-7Zr alloy Biofilm Bone marrow cells Osteogenesis |
topic |
Ti-35Nb-7Zr alloy Biofilm Bone marrow cells Osteogenesis |
description |
Background An effective biomaterial for bone replacement should have properties to avoid bacterial contamination and promote bone formation while inducing rapid cell differentiation simultaneously. Bone marrow stem cells are currently being investigated because of their known potential for differentiation in osteoblast lineage. This makes these cells a good option for stem cell-based therapy. We have aimed to analyze, in vitro, the potential of pure titanium (Ti), Ti-35Nb-7Zr alloy (A), niobium (Nb), and zirconia (Zr) to avoid the microorganisms S. aureus (S.a) and P. aeruginosa (P.a). Furthermore, our objective was to evaluate if the basic elements of Ti-35Nb-7Zr alloy have any influence on bone marrow stromal cells, the source of stem cells, and observe if these metals have properties to induce cell differentiation into osteoblasts. Methods Bone marrow stromal cells (BMSC) were obtained from mice femurs and cultured in osteogenic media without dexamethasone as an external source of cell differentiation. The samples were divided into Ti-35Nb-7Zr alloy (A), pure titanium (Ti), Nb (niobium), and Zr (zirconia) and were characterized by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). After predetermined periods, cell interaction, cytotoxicity, proliferation, and cell differentiation tests were performed. For monotypic biofilm formation, standardized suspensions (10(6) cells/ml) with the microorganisms S. aureus (S.a) and P. aeruginosa (P.a) were cultured for 24 h on the samples and submitted to an MTT test. Results All samples presented cell proliferation, growth, and spreading. All groups presented cell viability above 70%, but the alloy (A) showed better results, with statistical differences from Nb and Zr samples. Zr expressed higher ALP activity and was statistically different from the other groups (p < 0.05). In contrast, no statistical difference was observed between the samples as regards mineralization nodules. Lower biofilm formation of S.a and P.a. was observed on the Nb samples, with statistical differences from the other samples. Conclusion Our results suggest that the basic elements present in the alloy have osteoinductive characteristics, and Zr has a good influence on bone marrow stromal cell differentiation. We also believe that Nb has the best potential for reducing the formation of microbial biofilms. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-25 2021-06-25T12:23:19Z 2021-06-25T12:23:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s40729-020-00261-3 International Journal Of Implant Dentistry. Tokyo: Springer Japan Kk, v. 6, n. 1, 11 p., 2020. http://hdl.handle.net/11449/209590 10.1186/s40729-020-00261-3 WOS:000582333100001 |
url |
http://dx.doi.org/10.1186/s40729-020-00261-3 http://hdl.handle.net/11449/209590 |
identifier_str_mv |
International Journal Of Implant Dentistry. Tokyo: Springer Japan Kk, v. 6, n. 1, 11 p., 2020. 10.1186/s40729-020-00261-3 WOS:000582333100001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Implant Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789957428871168 |