Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3109/10641955.2015.1122034 http://hdl.handle.net/11449/176907 |
Resumo: | Although the role of soluble fms-like tyrosine kinase 1 (sFLT-1) in preeclampsia is well-established, the mechanism related to its synthesis remains poorly understood. We evaluated the association among the circulating microRNAs (miRs) and sFLT-1 levels in preeclampsia pregnant women. For this purpose, we measured the plasma sFLT-1 levels in 24 preeclampsia women and selected from these, three subjects with the lowest and three with the highest levels of sFLT-1 in order to screen for potential miRs associated with plasmatic sFLT-1 concentrations using a polymerase chain reaction-array (PCR-array) methodology. From screening results, we found three statistically different expressed miRs with fold change (FC-high/low levels) ≥3.0: miR-195-5p (FC = 5.2 increase in group with high sFLT-1 levels), miR-16-5p (FC = 3.2; increase in group with high sFLT-1 levels), and miR-375 (FC = −3.0; decrease in group with high sFLT-1 levels) which were later validated in all samples (n = 24). To correlate these miRs and plasma sFLT-1 levels, we used two extremes of analysis. In one part, we compared 12 preeclampsia women with the lowest sFLT-1 levels (L-50% group) to 12 with the highest levels (50% H group); and in the second analysis, 6 preeclampsia women (L-25%) from the L group to 6 preeclampsia women from the H group (H-25%). Our results showed increased expression of miR-195-5p in the H group, considering both the analyses with 50%, FC = 2.1 and 25%, FC = 4.2. Regarding other miRs, lack of correlation was found in both analyses (50% and 25%). In conclusion, our data demonstrate an association of higher levels of sFLT-1 with increased expression of miR-195-5p in preeclampsia pregnant women. |
id |
UNSP_c50cd0c59324f94d1635826f9ea9409a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/176907 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsiaCirculating microRNAmicroRNAmiR-195PCR-arraypreeclampsiasFLT-1Although the role of soluble fms-like tyrosine kinase 1 (sFLT-1) in preeclampsia is well-established, the mechanism related to its synthesis remains poorly understood. We evaluated the association among the circulating microRNAs (miRs) and sFLT-1 levels in preeclampsia pregnant women. For this purpose, we measured the plasma sFLT-1 levels in 24 preeclampsia women and selected from these, three subjects with the lowest and three with the highest levels of sFLT-1 in order to screen for potential miRs associated with plasmatic sFLT-1 concentrations using a polymerase chain reaction-array (PCR-array) methodology. From screening results, we found three statistically different expressed miRs with fold change (FC-high/low levels) ≥3.0: miR-195-5p (FC = 5.2 increase in group with high sFLT-1 levels), miR-16-5p (FC = 3.2; increase in group with high sFLT-1 levels), and miR-375 (FC = −3.0; decrease in group with high sFLT-1 levels) which were later validated in all samples (n = 24). To correlate these miRs and plasma sFLT-1 levels, we used two extremes of analysis. In one part, we compared 12 preeclampsia women with the lowest sFLT-1 levels (L-50% group) to 12 with the highest levels (50% H group); and in the second analysis, 6 preeclampsia women (L-25%) from the L group to 6 preeclampsia women from the H group (H-25%). Our results showed increased expression of miR-195-5p in the H group, considering both the analyses with 50%, FC = 2.1 and 25%, FC = 4.2. Regarding other miRs, lack of correlation was found in both analyses (50% and 25%). In conclusion, our data demonstrate an association of higher levels of sFLT-1 with increased expression of miR-195-5p in preeclampsia pregnant women.Department of Pharmacology Institute of Biosciences Universidade Estadual Paulista (UNESP)Núcleo de Pós-Graduação e Pesquisa – Santa Casa de Belo HorizonteDepartment of Pharmacology Faculty of Medicine of Ribeirao Preto University of Sao PauloDepartment of Gynecology and Obstetrics Faculty of Medicine of Ribeirao Preto University of Sao PauloDepartment of Pharmacology Institute of Biosciences Universidade Estadual Paulista (UNESP)Universidade Estadual Paulista (Unesp)Núcleo de Pós-Graduação e Pesquisa – Santa Casa de Belo HorizonteUniversidade de São Paulo (USP)Sandrim, Valeria Cristina [UNESP]Eleuterio, Nibia [UNESP]Pilan, Eliane [UNESP]Tanus-Santos, Jose EduardoFernandes, KarlaCavalli, Ricardo2018-12-11T17:23:03Z2018-12-11T17:23:03Z2016-04-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article150-158http://dx.doi.org/10.3109/10641955.2015.1122034Hypertension in Pregnancy, v. 35, n. 2, p. 150-158, 2016.1525-60651064-1955http://hdl.handle.net/11449/17690710.3109/10641955.2015.11220342-s2.0-84959055876Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHypertension in Pregnancy0,556info:eu-repo/semantics/openAccess2021-10-23T16:57:51Zoai:repositorio.unesp.br:11449/176907Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:57:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia |
title |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia |
spellingShingle |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia Sandrim, Valeria Cristina [UNESP] Circulating microRNA microRNA miR-195 PCR-array preeclampsia sFLT-1 |
title_short |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia |
title_full |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia |
title_fullStr |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia |
title_full_unstemmed |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia |
title_sort |
Plasma levels of increased miR-195-5p correlates with the sFLT-1 levels in preeclampsia |
author |
Sandrim, Valeria Cristina [UNESP] |
author_facet |
Sandrim, Valeria Cristina [UNESP] Eleuterio, Nibia [UNESP] Pilan, Eliane [UNESP] Tanus-Santos, Jose Eduardo Fernandes, Karla Cavalli, Ricardo |
author_role |
author |
author2 |
Eleuterio, Nibia [UNESP] Pilan, Eliane [UNESP] Tanus-Santos, Jose Eduardo Fernandes, Karla Cavalli, Ricardo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Núcleo de Pós-Graduação e Pesquisa – Santa Casa de Belo Horizonte Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Sandrim, Valeria Cristina [UNESP] Eleuterio, Nibia [UNESP] Pilan, Eliane [UNESP] Tanus-Santos, Jose Eduardo Fernandes, Karla Cavalli, Ricardo |
dc.subject.por.fl_str_mv |
Circulating microRNA microRNA miR-195 PCR-array preeclampsia sFLT-1 |
topic |
Circulating microRNA microRNA miR-195 PCR-array preeclampsia sFLT-1 |
description |
Although the role of soluble fms-like tyrosine kinase 1 (sFLT-1) in preeclampsia is well-established, the mechanism related to its synthesis remains poorly understood. We evaluated the association among the circulating microRNAs (miRs) and sFLT-1 levels in preeclampsia pregnant women. For this purpose, we measured the plasma sFLT-1 levels in 24 preeclampsia women and selected from these, three subjects with the lowest and three with the highest levels of sFLT-1 in order to screen for potential miRs associated with plasmatic sFLT-1 concentrations using a polymerase chain reaction-array (PCR-array) methodology. From screening results, we found three statistically different expressed miRs with fold change (FC-high/low levels) ≥3.0: miR-195-5p (FC = 5.2 increase in group with high sFLT-1 levels), miR-16-5p (FC = 3.2; increase in group with high sFLT-1 levels), and miR-375 (FC = −3.0; decrease in group with high sFLT-1 levels) which were later validated in all samples (n = 24). To correlate these miRs and plasma sFLT-1 levels, we used two extremes of analysis. In one part, we compared 12 preeclampsia women with the lowest sFLT-1 levels (L-50% group) to 12 with the highest levels (50% H group); and in the second analysis, 6 preeclampsia women (L-25%) from the L group to 6 preeclampsia women from the H group (H-25%). Our results showed increased expression of miR-195-5p in the H group, considering both the analyses with 50%, FC = 2.1 and 25%, FC = 4.2. Regarding other miRs, lack of correlation was found in both analyses (50% and 25%). In conclusion, our data demonstrate an association of higher levels of sFLT-1 with increased expression of miR-195-5p in preeclampsia pregnant women. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-02 2018-12-11T17:23:03Z 2018-12-11T17:23:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3109/10641955.2015.1122034 Hypertension in Pregnancy, v. 35, n. 2, p. 150-158, 2016. 1525-6065 1064-1955 http://hdl.handle.net/11449/176907 10.3109/10641955.2015.1122034 2-s2.0-84959055876 |
url |
http://dx.doi.org/10.3109/10641955.2015.1122034 http://hdl.handle.net/11449/176907 |
identifier_str_mv |
Hypertension in Pregnancy, v. 35, n. 2, p. 150-158, 2016. 1525-6065 1064-1955 10.3109/10641955.2015.1122034 2-s2.0-84959055876 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hypertension in Pregnancy 0,556 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
150-158 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789895758970880 |