Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jphotobiol.2017.11.027 http://hdl.handle.net/11449/170429 |
Resumo: | This study aims to evaluate, in vitro, the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled water; AL - Aloe vera with distilled water and FTL; HA - calcium hydroxide P.A. with distilled water; HL - calcium hydroxide P.A. with distilled water and FTL; HAA - calcium hydroxide P.A. with Aloe vera and distilled water; HAL - calcium hydroxide P.A. with Aloe vera, distilled water, and FTL. The cytotoxicity was evaluated by MTT assay at 24, 48, and 72 h and the genotoxicity by micronucleus test assay. This study was performed in triplicate. Data obtained in both tests were statistically analyzed by ANOVA and Tukey's tests (p ≤ 0.05). Group AA presented high genotoxicity and low cytotoxicity. After 24, 48, and 72 h, the group HAA significantly reduced the cell viability. Interaction with FTL showed slightly increase cell viability after 24 and 48 h in groups CL and HL (p < 0.001), despite the high genotoxicity in group CL and low genotoxicity in group HL. Group AL showed higher cell survival rate at 72 h (p < 0.05) and high genotoxicity (p < 0.001). It was concluded that Aloe vera allowed higher cell viability in human pulp fibroblasts in the presence of calcium hydroxide or with FTL separately, but genotoxicity increased in these associations. |
id |
UNSP_c5394a4cb12da1ec9ed86971d618fc59 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/170429 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulationAloe veraCytotoxicityEndodontic medicationGenotoxicityLaser photobiomodulationThis study aims to evaluate, in vitro, the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled water; AL - Aloe vera with distilled water and FTL; HA - calcium hydroxide P.A. with distilled water; HL - calcium hydroxide P.A. with distilled water and FTL; HAA - calcium hydroxide P.A. with Aloe vera and distilled water; HAL - calcium hydroxide P.A. with Aloe vera, distilled water, and FTL. The cytotoxicity was evaluated by MTT assay at 24, 48, and 72 h and the genotoxicity by micronucleus test assay. This study was performed in triplicate. Data obtained in both tests were statistically analyzed by ANOVA and Tukey's tests (p ≤ 0.05). Group AA presented high genotoxicity and low cytotoxicity. After 24, 48, and 72 h, the group HAA significantly reduced the cell viability. Interaction with FTL showed slightly increase cell viability after 24 and 48 h in groups CL and HL (p < 0.001), despite the high genotoxicity in group CL and low genotoxicity in group HL. Group AL showed higher cell survival rate at 72 h (p < 0.05) and high genotoxicity (p < 0.001). It was concluded that Aloe vera allowed higher cell viability in human pulp fibroblasts in the presence of calcium hydroxide or with FTL separately, but genotoxicity increased in these associations.School of Dentistry of Pernambuco University of PernambucoTiradentes UniversityFederal University of SergipeSão Paulo State University - UNESP, São José dos CamposSão Paulo State University - UNESP, São José dos CamposUniversity of PernambucoTiradentes UniversityUniversidade Federal de Sergipe (UFS)Universidade Estadual Paulista (Unesp)Carvalho, Nayane ChagasGuedes, Simone Alves GarcezAlbuquerque-Júnior, Ricardo Luiz Cavalcantide Albuquerque, Diana Santanade Souza Araújo, Adriano AntunesParanhos, Luiz RenatoCamargo, Samira Esteves Afonso [UNESP]Ribeiro, Maria Amália Gonzaga2018-12-11T16:50:45Z2018-12-11T16:50:45Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article348-354application/pdfhttp://dx.doi.org/10.1016/j.jphotobiol.2017.11.027Journal of Photochemistry and Photobiology B: Biology, v. 178, p. 348-354.1873-26821011-1344http://hdl.handle.net/11449/17042910.1016/j.jphotobiol.2017.11.0272-s2.0-850353635202-s2.0-85035363520.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Photochemistry and Photobiology B: Biology0,698info:eu-repo/semantics/openAccess2024-01-16T06:24:43Zoai:repositorio.unesp.br:11449/170429Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-16T06:24:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation |
title |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation |
spellingShingle |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation Carvalho, Nayane Chagas Aloe vera Cytotoxicity Endodontic medication Genotoxicity Laser photobiomodulation |
title_short |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation |
title_full |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation |
title_fullStr |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation |
title_full_unstemmed |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation |
title_sort |
Analysis of Aloe vera cytotoxicity and genotoxicity associated with endodontic medication and laser photobiomodulation |
author |
Carvalho, Nayane Chagas |
author_facet |
Carvalho, Nayane Chagas Guedes, Simone Alves Garcez Albuquerque-Júnior, Ricardo Luiz Cavalcanti de Albuquerque, Diana Santana de Souza Araújo, Adriano Antunes Paranhos, Luiz Renato Camargo, Samira Esteves Afonso [UNESP] Ribeiro, Maria Amália Gonzaga |
author_role |
author |
author2 |
Guedes, Simone Alves Garcez Albuquerque-Júnior, Ricardo Luiz Cavalcanti de Albuquerque, Diana Santana de Souza Araújo, Adriano Antunes Paranhos, Luiz Renato Camargo, Samira Esteves Afonso [UNESP] Ribeiro, Maria Amália Gonzaga |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Pernambuco Tiradentes University Universidade Federal de Sergipe (UFS) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Carvalho, Nayane Chagas Guedes, Simone Alves Garcez Albuquerque-Júnior, Ricardo Luiz Cavalcanti de Albuquerque, Diana Santana de Souza Araújo, Adriano Antunes Paranhos, Luiz Renato Camargo, Samira Esteves Afonso [UNESP] Ribeiro, Maria Amália Gonzaga |
dc.subject.por.fl_str_mv |
Aloe vera Cytotoxicity Endodontic medication Genotoxicity Laser photobiomodulation |
topic |
Aloe vera Cytotoxicity Endodontic medication Genotoxicity Laser photobiomodulation |
description |
This study aims to evaluate, in vitro, the effect of Aloe vera associated with endodontic medication, with or without laser photobiomodulation (FTL) irradiation in FP6 human pulp fibroblasts. The materials were divided into eight groups: CTR - control; CL - FTL alone; AA - Aloe vera with distilled water; AL - Aloe vera with distilled water and FTL; HA - calcium hydroxide P.A. with distilled water; HL - calcium hydroxide P.A. with distilled water and FTL; HAA - calcium hydroxide P.A. with Aloe vera and distilled water; HAL - calcium hydroxide P.A. with Aloe vera, distilled water, and FTL. The cytotoxicity was evaluated by MTT assay at 24, 48, and 72 h and the genotoxicity by micronucleus test assay. This study was performed in triplicate. Data obtained in both tests were statistically analyzed by ANOVA and Tukey's tests (p ≤ 0.05). Group AA presented high genotoxicity and low cytotoxicity. After 24, 48, and 72 h, the group HAA significantly reduced the cell viability. Interaction with FTL showed slightly increase cell viability after 24 and 48 h in groups CL and HL (p < 0.001), despite the high genotoxicity in group CL and low genotoxicity in group HL. Group AL showed higher cell survival rate at 72 h (p < 0.05) and high genotoxicity (p < 0.001). It was concluded that Aloe vera allowed higher cell viability in human pulp fibroblasts in the presence of calcium hydroxide or with FTL separately, but genotoxicity increased in these associations. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:50:45Z 2018-12-11T16:50:45Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jphotobiol.2017.11.027 Journal of Photochemistry and Photobiology B: Biology, v. 178, p. 348-354. 1873-2682 1011-1344 http://hdl.handle.net/11449/170429 10.1016/j.jphotobiol.2017.11.027 2-s2.0-85035363520 2-s2.0-85035363520.pdf |
url |
http://dx.doi.org/10.1016/j.jphotobiol.2017.11.027 http://hdl.handle.net/11449/170429 |
identifier_str_mv |
Journal of Photochemistry and Photobiology B: Biology, v. 178, p. 348-354. 1873-2682 1011-1344 10.1016/j.jphotobiol.2017.11.027 2-s2.0-85035363520 2-s2.0-85035363520.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Photochemistry and Photobiology B: Biology 0,698 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
348-354 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790307066052608 |