Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes
Autor(a) principal: | |
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Data de Publicação: | 1992 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/00032719208020053 http://hdl.handle.net/11449/230954 |
Resumo: | A graphite electrode assembled in a flow cell has shown to be a good detector for NADH. Current is linearly dependent on -7-3 concentration in the range 10 -710-3 M without any mediator at the potential applied of 300 mV vs Ag/AgCl. Lactate and alcohol dehydrogenases were immobilized near to the electrode surface or in a reactor to obtain an NADH-based biosensor for lactate or alcohol. With lactate we succeeded to obtain a response only if the reactor was used and for alcohol a current proportional to the concentration was obtained either if the enzyme was immobilized in a membrane and placed near the electrode surface or when the enzyme was immobilized in a reactor form. By FIA procedures we could obtain fast responses and recoveries, but with a short linear range. © 1992, Taylor & Francis Group, LLC. All rights reserved. |
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Repositório Institucional da UNESP |
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Nadh Electrochemical Sensor Coupled by Dehydrogenase EnzymesAlcoholBioreactorsDehydrogenaseEnzymesGraphite ElectrodesLactateNADHA graphite electrode assembled in a flow cell has shown to be a good detector for NADH. Current is linearly dependent on -7-3 concentration in the range 10 -710-3 M without any mediator at the potential applied of 300 mV vs Ag/AgCl. Lactate and alcohol dehydrogenases were immobilized near to the electrode surface or in a reactor to obtain an NADH-based biosensor for lactate or alcohol. With lactate we succeeded to obtain a response only if the reactor was used and for alcohol a current proportional to the concentration was obtained either if the enzyme was immobilized in a membrane and placed near the electrode surface or when the enzyme was immobilized in a reactor form. By FIA procedures we could obtain fast responses and recoveries, but with a short linear range. © 1992, Taylor & Francis Group, LLC. All rights reserved.Departamento de Quimica Analitica Instituto de Quimica, UNESP Caixa Postal 355, 14800 Araraquara (SP)Dipartimento di Sanita Pubblica Epidemiologia e Chimica Analitica Ambientale, Via Gino Capponi 9, 50121 FirenzeUniversidade Estadual Paulista (UNESP)Epidemiologia e Chimica Analitica AmbientaleYamanaka, HidekoMascini, Marco2022-04-29T08:42:52Z2022-04-29T08:42:52Z1992-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article983-997http://dx.doi.org/10.1080/00032719208020053Analytical Letters, v. 25, n. 6, p. 983-997, 1992.1532-236X0003-2719http://hdl.handle.net/11449/23095410.1080/000327192080200532-s2.0-0342432133Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnalytical Lettersinfo:eu-repo/semantics/openAccess2022-04-29T08:42:52Zoai:repositorio.unesp.br:11449/230954Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:42:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes |
title |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes |
spellingShingle |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes Yamanaka, Hideko Alcohol Bioreactors Dehydrogenase Enzymes Graphite Electrodes Lactate NADH |
title_short |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes |
title_full |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes |
title_fullStr |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes |
title_full_unstemmed |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes |
title_sort |
Nadh Electrochemical Sensor Coupled by Dehydrogenase Enzymes |
author |
Yamanaka, Hideko |
author_facet |
Yamanaka, Hideko Mascini, Marco |
author_role |
author |
author2 |
Mascini, Marco |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Epidemiologia e Chimica Analitica Ambientale |
dc.contributor.author.fl_str_mv |
Yamanaka, Hideko Mascini, Marco |
dc.subject.por.fl_str_mv |
Alcohol Bioreactors Dehydrogenase Enzymes Graphite Electrodes Lactate NADH |
topic |
Alcohol Bioreactors Dehydrogenase Enzymes Graphite Electrodes Lactate NADH |
description |
A graphite electrode assembled in a flow cell has shown to be a good detector for NADH. Current is linearly dependent on -7-3 concentration in the range 10 -710-3 M without any mediator at the potential applied of 300 mV vs Ag/AgCl. Lactate and alcohol dehydrogenases were immobilized near to the electrode surface or in a reactor to obtain an NADH-based biosensor for lactate or alcohol. With lactate we succeeded to obtain a response only if the reactor was used and for alcohol a current proportional to the concentration was obtained either if the enzyme was immobilized in a membrane and placed near the electrode surface or when the enzyme was immobilized in a reactor form. By FIA procedures we could obtain fast responses and recoveries, but with a short linear range. © 1992, Taylor & Francis Group, LLC. All rights reserved. |
publishDate |
1992 |
dc.date.none.fl_str_mv |
1992-06-01 2022-04-29T08:42:52Z 2022-04-29T08:42:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/00032719208020053 Analytical Letters, v. 25, n. 6, p. 983-997, 1992. 1532-236X 0003-2719 http://hdl.handle.net/11449/230954 10.1080/00032719208020053 2-s2.0-0342432133 |
url |
http://dx.doi.org/10.1080/00032719208020053 http://hdl.handle.net/11449/230954 |
identifier_str_mv |
Analytical Letters, v. 25, n. 6, p. 983-997, 1992. 1532-236X 0003-2719 10.1080/00032719208020053 2-s2.0-0342432133 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Analytical Letters |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
983-997 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964852254408704 |