Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii
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Publication Date: | 2007 |
Other Authors: | , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1051/vetres:2007023 http://hdl.handle.net/11449/37501 |
Summary: | The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the São Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% ( 4/197) and 1.5% ( 3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer ( ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples ( n = 8) were inoculated into a liquid pre-enrichment growth medium ( BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog ( ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii ( pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs. |
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Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffiidogsBartonella infectionsheart diseasecultureBrazilThe purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the São Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% ( 4/197) and 1.5% ( 3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer ( ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples ( n = 8) were inoculated into a liquid pre-enrichment growth medium ( BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog ( ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii ( pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs.N Carolina State Univ, Sch Vet Med, Ctr Comparat Med & Translat Res, Dept Clin Sci,Intracellular Pathogens Res Lab, Raleigh, NC 27606 USAUniv Estadual Paulista, FMVZ, Sch Vet Med & Anim Sci, São Paulo, BrazilUniv Estadual Paulista, FMVZ, Sch Vet Med & Anim Sci, São Paulo, BrazilEdp Sciences S AN Carolina State UnivUniversidade Estadual Paulista (Unesp)Vissotto de Paiva Diniz, Pedro PauloMaggi, Ricardo GuillermoSchwartz, Denise SarettaCadenas, Maria BelenBradley, Julie MeredithHegarty, BarbaraBreitschwerdt, Edward Bealmear2014-05-20T15:27:32Z2014-05-20T15:27:32Z2007-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article697-710application/pdfhttp://dx.doi.org/10.1051/vetres:2007023Veterinary Research. Les Ulis Cedex A: Edp Sciences S A, v. 38, n. 5, p. 697-710, 2007.0928-4249http://hdl.handle.net/11449/3750110.1051/vetres:2007023WOS:000249779400004WOS000249779400004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Research2.9031,266info:eu-repo/semantics/openAccess2023-12-24T06:13:14Zoai:repositorio.unesp.br:11449/37501Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-24T06:13:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii |
title |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii |
spellingShingle |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii Vissotto de Paiva Diniz, Pedro Paulo dogs Bartonella infections heart disease culture Brazil |
title_short |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii |
title_full |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii |
title_fullStr |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii |
title_full_unstemmed |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii |
title_sort |
Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp berkhoffii |
author |
Vissotto de Paiva Diniz, Pedro Paulo |
author_facet |
Vissotto de Paiva Diniz, Pedro Paulo Maggi, Ricardo Guillermo Schwartz, Denise Saretta Cadenas, Maria Belen Bradley, Julie Meredith Hegarty, Barbara Breitschwerdt, Edward Bealmear |
author_role |
author |
author2 |
Maggi, Ricardo Guillermo Schwartz, Denise Saretta Cadenas, Maria Belen Bradley, Julie Meredith Hegarty, Barbara Breitschwerdt, Edward Bealmear |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
N Carolina State Univ Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vissotto de Paiva Diniz, Pedro Paulo Maggi, Ricardo Guillermo Schwartz, Denise Saretta Cadenas, Maria Belen Bradley, Julie Meredith Hegarty, Barbara Breitschwerdt, Edward Bealmear |
dc.subject.por.fl_str_mv |
dogs Bartonella infections heart disease culture Brazil |
topic |
dogs Bartonella infections heart disease culture Brazil |
description |
The purpose of this study was to determine the serological and molecular prevalence of Bartonella spp. infection in a sick dog population from Brazil. At the São Paulo State University Veterinary Teaching Hospital in Botucatu, 198 consecutive dogs with clinicopathological abnormalities consistent with tick-borne infections were sampled. Antibodies to Bartonella henselae and Bartonella vinsonii subsp. berkhoffii were detected in 2.0% ( 4/197) and 1.5% ( 3/197) of the dogs, respectively. Using 16S-23S rRNA intergenic transcribed spacer ( ITS) primers, Bartonella DNA was amplified from only 1/198 blood samples. Bartonella seroreactive and/or PCR positive blood samples ( n = 8) were inoculated into a liquid pre-enrichment growth medium ( BAPGM) and subsequently sub-inoculated onto BAPGM/blood-agar plates. PCR targeting the ITS region, pap31 and rpoB genes amplified B. henselae from the blood and/or isolates of the PCR positive dog ( ITS: DQ346666; pap31 gene: DQ351240; rpoB: EF196806). B. henselae and B. vinsonii subsp. berkhoffii ( pap31: DQ906160; rpoB: EF196805) co-infection was found in one of the B. vinsonii subsp. berkhoffii seroreactive dogs. We conclude that dogs in this study population were infrequently exposed to or infected with a Bartonella species. The B. henselae and B. vinsonii subsp. berkhoffii strains identified in this study are genetically similar to strains isolated from septicemic cats, dogs, coyotes and human beings from other parts of the world. To our knowledge, these isolates provide the first Brazilian DNA sequences from these Bartonella species and the first evidence of Bartonella co-infection in dogs. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-09-01 2014-05-20T15:27:32Z 2014-05-20T15:27:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1051/vetres:2007023 Veterinary Research. Les Ulis Cedex A: Edp Sciences S A, v. 38, n. 5, p. 697-710, 2007. 0928-4249 http://hdl.handle.net/11449/37501 10.1051/vetres:2007023 WOS:000249779400004 WOS000249779400004.pdf |
url |
http://dx.doi.org/10.1051/vetres:2007023 http://hdl.handle.net/11449/37501 |
identifier_str_mv |
Veterinary Research. Les Ulis Cedex A: Edp Sciences S A, v. 38, n. 5, p. 697-710, 2007. 0928-4249 10.1051/vetres:2007023 WOS:000249779400004 WOS000249779400004.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Research 2.903 1,266 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
697-710 application/pdf |
dc.publisher.none.fl_str_mv |
Edp Sciences S A |
publisher.none.fl_str_mv |
Edp Sciences S A |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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