Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial

Detalhes bibliográficos
Autor(a) principal: Silva, Mariana Fedocci [UNESP]
Data de Publicação: 2015
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/139015
http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2016-05-10/000865321.pdf
Resumo: The action of man has led, over the years, major impacts on the environment, especially in aquatic ecosystems, leading to an impairment of water quality, considered one of the essential factors for the maintenance of vital functions and consequently the life of the planet. Among the activities considered a risk for the environment are linked to pollution from many sources and even entire watersheds, whether by industrial waste, sewage, or for substances used in agriculture, such as pesticides, herbicides and fertilizers. The (2,4-D) 2,4- dichlorophenoxyacetic acid is used worldwide, and the fact that its genotoxicity is proven by several studies and by its long persistence in soil, which enables the leaching and percolation of compounds affecting water bodies, toxicity studies are relevant and justifiable. Thus, this study aimed to evaluate the toxicity of 2,4-D by examining the liver of the fish Oreochromis niloticus exposed to different dilutions. Portions of liver were collected and fixed for histological and histochemical techniques to detect total proteins, polysaccharides and lipids. lipids. Treatment with 2,4-D herbicide apparently did not alter the lipid profiles, the accumulation of polysaccharides, and the presence of total proteins. The 2.5 and 5.0% were lethal to fish. These mortalities are probably of high toxic and cytotoxic potential of 2,4-D herbicide results. Several histopathological changes were found, such as: loss of cytoplasmic integrity, loss of cell limit, nuclear deformation, vacuolated cytoplasm, tissue disorganization and hydropic degeneration. Statistically significant changes were: hydropic degeneration and vacuolated cytoplasm. It is concluded, therefore, that the qualitative morphological analysis is an important method for observing changes in liver toxicology studies. As the O. niloticus species is an efficient biological indicator of water pollution by 2,4-D
id UNSP_c5a61ce393f016a1ec9801a805efb0f6
oai_identifier_str oai:repositorio.unesp.br:11449/139015
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercialEcologia aquaticaHistopatologia veterinariaTilapia (Peixe)VenenosÁgua - PoluiçãoThe action of man has led, over the years, major impacts on the environment, especially in aquatic ecosystems, leading to an impairment of water quality, considered one of the essential factors for the maintenance of vital functions and consequently the life of the planet. Among the activities considered a risk for the environment are linked to pollution from many sources and even entire watersheds, whether by industrial waste, sewage, or for substances used in agriculture, such as pesticides, herbicides and fertilizers. The (2,4-D) 2,4- dichlorophenoxyacetic acid is used worldwide, and the fact that its genotoxicity is proven by several studies and by its long persistence in soil, which enables the leaching and percolation of compounds affecting water bodies, toxicity studies are relevant and justifiable. Thus, this study aimed to evaluate the toxicity of 2,4-D by examining the liver of the fish Oreochromis niloticus exposed to different dilutions. Portions of liver were collected and fixed for histological and histochemical techniques to detect total proteins, polysaccharides and lipids. lipids. Treatment with 2,4-D herbicide apparently did not alter the lipid profiles, the accumulation of polysaccharides, and the presence of total proteins. The 2.5 and 5.0% were lethal to fish. These mortalities are probably of high toxic and cytotoxic potential of 2,4-D herbicide results. Several histopathological changes were found, such as: loss of cytoplasmic integrity, loss of cell limit, nuclear deformation, vacuolated cytoplasm, tissue disorganization and hydropic degeneration. Statistically significant changes were: hydropic degeneration and vacuolated cytoplasm. It is concluded, therefore, that the qualitative morphological analysis is an important method for observing changes in liver toxicology studies. As the O. niloticus species is an efficient biological indicator of water pollution by 2,4-DAo longo dos anos, a ação do homem tem provocado grande impacto no meio ambiente, principalmente em ecossistemas aquáticos, levando-o ao comprometimento da sua qualidade, uma vez que a água é considerada um dos fatores essenciais para a manutenção das funções vitais e, consequentemente, da vida do planeta. Entre as atividades consideradas de risco para o meio ambiente, estão as ligadas a poluição de muitos mananciais e até bacias hidrográficas inteiras, seja por resíduos industriais, esgoto doméstico, ou ainda, por substâncias utilizadas na agricultura, tais como pesticidas, herbicidas e fertilizantes. O ácido 2,4- diclorofenoxiacético (2,4-D) é usado em larga escala no mundo todo, e pelo fato de ter uma longa persistência no solo, o que possibilita a lixiviação e a percolação do composto afetando corpos hídricos, estudos de sua toxicidade são relevantes e justificáveis. Desta maneira, este estudo procurou avaliar a toxicidade do herbicida 2,4-D por meio da análise de fígados de peixes Oreochromis niloticus, expostos a diferentes diluições. Porções dos fígados foram coletadas e fixadas para serem analisadas por meio de técnicas histológica e histoquímicas para detecção de proteínas totais, polissacarídeos e lipídios. O tratamento com o herbicida 2,4-D aparentemente não alterou o padrão lipídico, o acúmulo de polissacarídeos e a presença de proteínas totais. As concentrações 2,5 e 5,0% foram letais aos peixes. Tais mortalidades provavelmente são resultados do alto potencial tóxico e citotóxico do herbicida 2,4-D. Foram encontradas diversas alterações histopatológicas, sendo elas: perda da integridade citoplasmática, perda do limite celular, deformação nuclear, citoplasma vacuolizado, desorganização do tecido e degeneração hidrópica. As alterações estatisticamente significativas foram: degeneração hidrópica e citoplasma vacuolizado. Concluiu-se, portanto, que a análise...Universidade Estadual Paulista (Unesp)Christofoletti, Carmem Silvia Fontanetti [UNESP]Marcato, Ana Claudia de Castro [UNESP]Universidade Estadual Paulista (Unesp)Silva, Mariana Fedocci [UNESP]2016-06-07T17:10:18Z2016-06-07T17:10:18Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesis34 f.application/pdfSILVA, Mariana Fedocci. Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial. 2015. 34 f. Trabalho de conclusão de curso (Ecologia) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2015.http://hdl.handle.net/11449/139015000865321http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2016-05-10/000865321.pdfAlephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-11-27T06:13:39Zoai:repositorio.unesp.br:11449/139015Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-27T06:13:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
title Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
spellingShingle Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
Silva, Mariana Fedocci [UNESP]
Ecologia aquatica
Histopatologia veterinaria
Tilapia (Peixe)
Venenos
Água - Poluição
title_short Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
title_full Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
title_fullStr Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
title_full_unstemmed Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
title_sort Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial
author Silva, Mariana Fedocci [UNESP]
author_facet Silva, Mariana Fedocci [UNESP]
author_role author
dc.contributor.none.fl_str_mv Christofoletti, Carmem Silvia Fontanetti [UNESP]
Marcato, Ana Claudia de Castro [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Mariana Fedocci [UNESP]
dc.subject.por.fl_str_mv Ecologia aquatica
Histopatologia veterinaria
Tilapia (Peixe)
Venenos
Água - Poluição
topic Ecologia aquatica
Histopatologia veterinaria
Tilapia (Peixe)
Venenos
Água - Poluição
description The action of man has led, over the years, major impacts on the environment, especially in aquatic ecosystems, leading to an impairment of water quality, considered one of the essential factors for the maintenance of vital functions and consequently the life of the planet. Among the activities considered a risk for the environment are linked to pollution from many sources and even entire watersheds, whether by industrial waste, sewage, or for substances used in agriculture, such as pesticides, herbicides and fertilizers. The (2,4-D) 2,4- dichlorophenoxyacetic acid is used worldwide, and the fact that its genotoxicity is proven by several studies and by its long persistence in soil, which enables the leaching and percolation of compounds affecting water bodies, toxicity studies are relevant and justifiable. Thus, this study aimed to evaluate the toxicity of 2,4-D by examining the liver of the fish Oreochromis niloticus exposed to different dilutions. Portions of liver were collected and fixed for histological and histochemical techniques to detect total proteins, polysaccharides and lipids. lipids. Treatment with 2,4-D herbicide apparently did not alter the lipid profiles, the accumulation of polysaccharides, and the presence of total proteins. The 2.5 and 5.0% were lethal to fish. These mortalities are probably of high toxic and cytotoxic potential of 2,4-D herbicide results. Several histopathological changes were found, such as: loss of cytoplasmic integrity, loss of cell limit, nuclear deformation, vacuolated cytoplasm, tissue disorganization and hydropic degeneration. Statistically significant changes were: hydropic degeneration and vacuolated cytoplasm. It is concluded, therefore, that the qualitative morphological analysis is an important method for observing changes in liver toxicology studies. As the O. niloticus species is an efficient biological indicator of water pollution by 2,4-D
publishDate 2015
dc.date.none.fl_str_mv 2015
2016-06-07T17:10:18Z
2016-06-07T17:10:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
format bachelorThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Mariana Fedocci. Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial. 2015. 34 f. Trabalho de conclusão de curso (Ecologia) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2015.
http://hdl.handle.net/11449/139015
000865321
http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2016-05-10/000865321.pdf
identifier_str_mv SILVA, Mariana Fedocci. Análise histopatológica de fígado de tilápias (Oreochromis Niloticus) expostas ao herbicida 2,4-D comercial. 2015. 34 f. Trabalho de conclusão de curso (Ecologia) - Universidade Estadual Paulista, Instituto de Biociências de Rio Claro, 2015.
000865321
url http://hdl.handle.net/11449/139015
http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2016-05-10/000865321.pdf
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 34 f.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965087312642048

Registros relacionados