A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses

Detalhes bibliográficos
Autor(a) principal: Alves da Cunha Valini, Graziela [UNESP]
Data de Publicação: 2023
Outros Autores: Arnaut, Pedro Righetti [UNESP], Barbosa, Larissa Gonçalves [UNESP], de Azevedo, Paulo Henrique Amadeu [UNESP], Melo, Antonio Diego Brandão [UNESP], Marçal, Danilo Alves [UNESP], Campos, Paulo Henrique Reis Furtado, Hauschild, Luciano [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/microorganisms11020446
http://hdl.handle.net/11449/248427
Resumo: Salmonella Typhimurium is the most frequent serovar in pigs and causes infections in humans. However, the dosage used for experimentation is not well defined. The present study aimed to evaluate a dosage for oral inoculation with Salmonella Typhimurium to assess immunological and growth performance alterations in pigs. Gilts were randomly allocated into one of three experimental treatments: no Salmonella Typhimurium inoculation (Basal), or oral inoculation of 1 × 108 or 1.5 × 108 colony-forming units of Salmonella Typhimurium. Growth rate, rectal temperature, and fecal Salmonella shedding were recorded. Blood samples were taken. Inoculated pigs shed the bacteria for up to 7 days, but no differences were observed between the groups. No differences were observed in rectal temperature, body weight, or average daily feed intake. However, reductions in average daily gain (−17 and −22%) and feed efficiency (−14 and −20%) were observed in pigs inoculated with 1 × 108 and 1.5 × 108 colony-forming units, respectively. The hemoglobin and hematocrit concentrations increased in challenged pigs compared to Basal pigs. The oral dosage of 1.5 × 108 colony-forming units of Salmonella Typhimurium is suitable for activating the immune system of pigs and assessing the impact of Salmonella on pig performance.
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spelling A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Dosesgrowthimmunologic responsesanitary challengeswineSalmonella Typhimurium is the most frequent serovar in pigs and causes infections in humans. However, the dosage used for experimentation is not well defined. The present study aimed to evaluate a dosage for oral inoculation with Salmonella Typhimurium to assess immunological and growth performance alterations in pigs. Gilts were randomly allocated into one of three experimental treatments: no Salmonella Typhimurium inoculation (Basal), or oral inoculation of 1 × 108 or 1.5 × 108 colony-forming units of Salmonella Typhimurium. Growth rate, rectal temperature, and fecal Salmonella shedding were recorded. Blood samples were taken. Inoculated pigs shed the bacteria for up to 7 days, but no differences were observed between the groups. No differences were observed in rectal temperature, body weight, or average daily feed intake. However, reductions in average daily gain (−17 and −22%) and feed efficiency (−14 and −20%) were observed in pigs inoculated with 1 × 108 and 1.5 × 108 colony-forming units, respectively. The hemoglobin and hematocrit concentrations increased in challenged pigs compared to Basal pigs. The oral dosage of 1.5 × 108 colony-forming units of Salmonella Typhimurium is suitable for activating the immune system of pigs and assessing the impact of Salmonella on pig performance.Department of Animal Science Faculdade de Ciências Agrárias e Veterinárias UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho”, São PauloDepartment of Animal Science Universidade Federal de Viçosa, Minas GeraisDepartment of Animal Science Faculdade de Ciências Agrárias e Veterinárias UNESP Universidade Estadual Paulista “Júlio de Mesquita Filho”, São PauloUniversidade Estadual Paulista (UNESP)Universidade Federal de Viçosa (UFV)Alves da Cunha Valini, Graziela [UNESP]Arnaut, Pedro Righetti [UNESP]Barbosa, Larissa Gonçalves [UNESP]de Azevedo, Paulo Henrique Amadeu [UNESP]Melo, Antonio Diego Brandão [UNESP]Marçal, Danilo Alves [UNESP]Campos, Paulo Henrique Reis FurtadoHauschild, Luciano [UNESP]2023-07-29T13:43:44Z2023-07-29T13:43:44Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/microorganisms11020446Microorganisms, v. 11, n. 2, 2023.2076-2607http://hdl.handle.net/11449/24842710.3390/microorganisms110204462-s2.0-85149013083Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMicroorganismsinfo:eu-repo/semantics/openAccess2023-07-29T13:43:44Zoai:repositorio.unesp.br:11449/248427Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:43:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
title A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
spellingShingle A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
Alves da Cunha Valini, Graziela [UNESP]
growth
immunologic response
sanitary challenge
swine
title_short A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
title_full A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
title_fullStr A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
title_full_unstemmed A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
title_sort A Simple Assay to Assess Salmonella Typhimurium Impact on Performance and Immune Status of Growing Pigs after Different Inoculation Doses
author Alves da Cunha Valini, Graziela [UNESP]
author_facet Alves da Cunha Valini, Graziela [UNESP]
Arnaut, Pedro Righetti [UNESP]
Barbosa, Larissa Gonçalves [UNESP]
de Azevedo, Paulo Henrique Amadeu [UNESP]
Melo, Antonio Diego Brandão [UNESP]
Marçal, Danilo Alves [UNESP]
Campos, Paulo Henrique Reis Furtado
Hauschild, Luciano [UNESP]
author_role author
author2 Arnaut, Pedro Righetti [UNESP]
Barbosa, Larissa Gonçalves [UNESP]
de Azevedo, Paulo Henrique Amadeu [UNESP]
Melo, Antonio Diego Brandão [UNESP]
Marçal, Danilo Alves [UNESP]
Campos, Paulo Henrique Reis Furtado
Hauschild, Luciano [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de Viçosa (UFV)
dc.contributor.author.fl_str_mv Alves da Cunha Valini, Graziela [UNESP]
Arnaut, Pedro Righetti [UNESP]
Barbosa, Larissa Gonçalves [UNESP]
de Azevedo, Paulo Henrique Amadeu [UNESP]
Melo, Antonio Diego Brandão [UNESP]
Marçal, Danilo Alves [UNESP]
Campos, Paulo Henrique Reis Furtado
Hauschild, Luciano [UNESP]
dc.subject.por.fl_str_mv growth
immunologic response
sanitary challenge
swine
topic growth
immunologic response
sanitary challenge
swine
description Salmonella Typhimurium is the most frequent serovar in pigs and causes infections in humans. However, the dosage used for experimentation is not well defined. The present study aimed to evaluate a dosage for oral inoculation with Salmonella Typhimurium to assess immunological and growth performance alterations in pigs. Gilts were randomly allocated into one of three experimental treatments: no Salmonella Typhimurium inoculation (Basal), or oral inoculation of 1 × 108 or 1.5 × 108 colony-forming units of Salmonella Typhimurium. Growth rate, rectal temperature, and fecal Salmonella shedding were recorded. Blood samples were taken. Inoculated pigs shed the bacteria for up to 7 days, but no differences were observed between the groups. No differences were observed in rectal temperature, body weight, or average daily feed intake. However, reductions in average daily gain (−17 and −22%) and feed efficiency (−14 and −20%) were observed in pigs inoculated with 1 × 108 and 1.5 × 108 colony-forming units, respectively. The hemoglobin and hematocrit concentrations increased in challenged pigs compared to Basal pigs. The oral dosage of 1.5 × 108 colony-forming units of Salmonella Typhimurium is suitable for activating the immune system of pigs and assessing the impact of Salmonella on pig performance.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:43:44Z
2023-07-29T13:43:44Z
2023-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/microorganisms11020446
Microorganisms, v. 11, n. 2, 2023.
2076-2607
http://hdl.handle.net/11449/248427
10.3390/microorganisms11020446
2-s2.0-85149013083
url http://dx.doi.org/10.3390/microorganisms11020446
http://hdl.handle.net/11449/248427
identifier_str_mv Microorganisms, v. 11, n. 2, 2023.
2076-2607
10.3390/microorganisms11020446
2-s2.0-85149013083
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964938255466496

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