Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0065 http://hdl.handle.net/11449/240045 |
Resumo: | Solubility, pH, ion release, cytotoxicity, and osteoclastogenesis inhibition in bone marrow-derived monocyte macrophages (BMMs) were evaluated in EndoSequence BC Sealer (END), Bio-C Sealer (BC), and Sealer Plus BC (SPBC). pH was determined after immersion of the sealers in deionized water (DW) and Minimum Essential Medium Alpha (α-MEM). Solubility was obtained by mass loss. Ion release was measured by using X-ray fluorescence spectroscopy (XRF). Cytotoxicity was evaluated by MTT assay. Inhibition of osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP). Data were analyzed using the t-test, ANOVA and Tukey/Dunnett’s post-hoc tests (α = 0.05). END had the highest pH in DW (p < 0.05), and BC, in α-MEM (p < 0.05). Solubility in DW was the lowest for SPBC (p < 0.005). The highest calcium release was observed for BC in DW at 12 h (p < 0.05), and in α-MEM at 12 and 24 h (p < 0.05). The lowest toxicity was detected for END (p < 0.05). BC had the highest inhibitory effect on osteoclasts (p < 0.05). Overall, the highest solubility and pH values were found in DW. However, the calcium silicate-based sealer showed higher solubility than the ISO standards. Calcium release was the highest for BC. END showed the highest cell viability, and BC, the highest osteoclast inhibition. |
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Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post setEndodonticsMaterials testingOsteogenesisRoot canal filling materialsSolubility, pH, ion release, cytotoxicity, and osteoclastogenesis inhibition in bone marrow-derived monocyte macrophages (BMMs) were evaluated in EndoSequence BC Sealer (END), Bio-C Sealer (BC), and Sealer Plus BC (SPBC). pH was determined after immersion of the sealers in deionized water (DW) and Minimum Essential Medium Alpha (α-MEM). Solubility was obtained by mass loss. Ion release was measured by using X-ray fluorescence spectroscopy (XRF). Cytotoxicity was evaluated by MTT assay. Inhibition of osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP). Data were analyzed using the t-test, ANOVA and Tukey/Dunnett’s post-hoc tests (α = 0.05). END had the highest pH in DW (p < 0.05), and BC, in α-MEM (p < 0.05). Solubility in DW was the lowest for SPBC (p < 0.005). The highest calcium release was observed for BC in DW at 12 h (p < 0.05), and in α-MEM at 12 and 24 h (p < 0.05). The lowest toxicity was detected for END (p < 0.05). BC had the highest inhibitory effect on osteoclasts (p < 0.05). Overall, the highest solubility and pH values were found in DW. However, the calcium silicate-based sealer showed higher solubility than the ISO standards. Calcium release was the highest for BC. END showed the highest cell viability, and BC, the highest osteoclast inhibition.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Federal de Uberlândia – UFU School of Dentistry Department of Endodontics, MGUniversidade Estadual Paulista – Unesp School of Dentistry Department of Restorative Dentistry, SPUniversidade Federal de Uberlândia – UFU Institute of Biomedical Sciences Department of Immunology, MGUniversidade Estadual Paulista – Unesp School of Dentistry Department of Restorative Dentistry, SPUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (UNESP)De Rosatto, Camila Maria PeresSouza, Gabriela Leite deFerraz, Danilo Cassiano [UNESP]Silva, Marcelo José BarbosaTanomaru-Filho, Mario [UNESP]Moura, Camilla Christian Gomes2023-03-01T19:58:57Z2023-03-01T19:58:57Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0065Brazilian Oral Research, v. 36.1807-31071806-8324http://hdl.handle.net/11449/24004510.1590/1807-3107bor-2022.vol36.00652-s2.0-85129929766Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Oral Researchinfo:eu-repo/semantics/openAccess2023-03-01T19:58:57Zoai:repositorio.unesp.br:11449/240045Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T19:58:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set |
title |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set |
spellingShingle |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set De Rosatto, Camila Maria Peres Endodontics Materials testing Osteogenesis Root canal filling materials |
title_short |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set |
title_full |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set |
title_fullStr |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set |
title_full_unstemmed |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set |
title_sort |
Physicochemical properties and osteoclastogenesis for three premixed calcium silicate-based sealers post set |
author |
De Rosatto, Camila Maria Peres |
author_facet |
De Rosatto, Camila Maria Peres Souza, Gabriela Leite de Ferraz, Danilo Cassiano [UNESP] Silva, Marcelo José Barbosa Tanomaru-Filho, Mario [UNESP] Moura, Camilla Christian Gomes |
author_role |
author |
author2 |
Souza, Gabriela Leite de Ferraz, Danilo Cassiano [UNESP] Silva, Marcelo José Barbosa Tanomaru-Filho, Mario [UNESP] Moura, Camilla Christian Gomes |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
De Rosatto, Camila Maria Peres Souza, Gabriela Leite de Ferraz, Danilo Cassiano [UNESP] Silva, Marcelo José Barbosa Tanomaru-Filho, Mario [UNESP] Moura, Camilla Christian Gomes |
dc.subject.por.fl_str_mv |
Endodontics Materials testing Osteogenesis Root canal filling materials |
topic |
Endodontics Materials testing Osteogenesis Root canal filling materials |
description |
Solubility, pH, ion release, cytotoxicity, and osteoclastogenesis inhibition in bone marrow-derived monocyte macrophages (BMMs) were evaluated in EndoSequence BC Sealer (END), Bio-C Sealer (BC), and Sealer Plus BC (SPBC). pH was determined after immersion of the sealers in deionized water (DW) and Minimum Essential Medium Alpha (α-MEM). Solubility was obtained by mass loss. Ion release was measured by using X-ray fluorescence spectroscopy (XRF). Cytotoxicity was evaluated by MTT assay. Inhibition of osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP). Data were analyzed using the t-test, ANOVA and Tukey/Dunnett’s post-hoc tests (α = 0.05). END had the highest pH in DW (p < 0.05), and BC, in α-MEM (p < 0.05). Solubility in DW was the lowest for SPBC (p < 0.005). The highest calcium release was observed for BC in DW at 12 h (p < 0.05), and in α-MEM at 12 and 24 h (p < 0.05). The lowest toxicity was detected for END (p < 0.05). BC had the highest inhibitory effect on osteoclasts (p < 0.05). Overall, the highest solubility and pH values were found in DW. However, the calcium silicate-based sealer showed higher solubility than the ISO standards. Calcium release was the highest for BC. END showed the highest cell viability, and BC, the highest osteoclast inhibition. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T19:58:57Z 2023-03-01T19:58:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0065 Brazilian Oral Research, v. 36. 1807-3107 1806-8324 http://hdl.handle.net/11449/240045 10.1590/1807-3107bor-2022.vol36.0065 2-s2.0-85129929766 |
url |
http://dx.doi.org/10.1590/1807-3107bor-2022.vol36.0065 http://hdl.handle.net/11449/240045 |
identifier_str_mv |
Brazilian Oral Research, v. 36. 1807-3107 1806-8324 10.1590/1807-3107bor-2022.vol36.0065 2-s2.0-85129929766 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Oral Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789272227446784 |