Toxicity and effect of whitening toothpastes on enamel surface
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0025 http://hdl.handle.net/11449/208463 |
Resumo: | This in vitro study evaluated the biocompatibility and abrasivity of whitening and conventional toothpastes. Samples of conventional (non-whitening) - Edel White Infant (EWI) - and whitening toothpastes - Edel White Whitening (EWW), Edel White CAREFORTE (EWC), Colgate Total 12 Ò Professional (C), and Oral-B Whitening (OB) - were dissolved in culture medium (0.2 g sample weight per mL). Human gingival fibroblasts (hGF) were placed in contact with different dilutions of culture media that had been previously exposed to these toothpastes. Cytotoxicity was then assessed using the methyl tetrazolium test (MTT) and the cell survival rate was determined. Genotoxicity was assessed by the micronucleus test (MNT) and the number of micronuclei was determined before and after exposure to the toothpaste solutions. The enamel surface roughness was evaluated in specimens of bovine teeth (n = 10 per group) before and after 10,000 brushing cycles, using the investigated toothpastes. The results were statistically analyzed using the Mann-Whitney U test and two-way ANOVA (p < 0.05). According to the MTT assay, EWW and OB presented significant cytotoxicity (p < 0.01), but no genotoxic (MNT) effects (p > 0.05). C toothpaste was statistically significantly abrasive to the enamel surface (p < 0.01). The findings of this study may be helpful for individualized selection of commercial toothpastes, as some whitening toothpastes present significant cytotoxicity and conventional toothpaste cause significant surface changes. |
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Toxicity and effect of whitening toothpastes on enamel surfaceThis in vitro study evaluated the biocompatibility and abrasivity of whitening and conventional toothpastes. Samples of conventional (non-whitening) - Edel White Infant (EWI) - and whitening toothpastes - Edel White Whitening (EWW), Edel White CAREFORTE (EWC), Colgate Total 12 Ò Professional (C), and Oral-B Whitening (OB) - were dissolved in culture medium (0.2 g sample weight per mL). Human gingival fibroblasts (hGF) were placed in contact with different dilutions of culture media that had been previously exposed to these toothpastes. Cytotoxicity was then assessed using the methyl tetrazolium test (MTT) and the cell survival rate was determined. Genotoxicity was assessed by the micronucleus test (MNT) and the number of micronuclei was determined before and after exposure to the toothpaste solutions. The enamel surface roughness was evaluated in specimens of bovine teeth (n = 10 per group) before and after 10,000 brushing cycles, using the investigated toothpastes. The results were statistically analyzed using the Mann-Whitney U test and two-way ANOVA (p < 0.05). According to the MTT assay, EWW and OB presented significant cytotoxicity (p < 0.01), but no genotoxic (MNT) effects (p > 0.05). C toothpaste was statistically significantly abrasive to the enamel surface (p < 0.01). The findings of this study may be helpful for individualized selection of commercial toothpastes, as some whitening toothpastes present significant cytotoxicity and conventional toothpaste cause significant surface changes.Universidade Estadual Paulista - Unesp Institute of Science and Technology Department of Dental MaterialsUniversidade Federal de Uberlândia - UFU School of Dentistry Postgraduate Program in Dentistry MGUniversidade Estadual Paulista - Unesp Institute of Science and Technology Department of Restorative DentistryUniversity of Florida College of Dentistry Department of Restorative Dental Sciences FLUniversidade Estadual Paulista - Unesp Institute of Science and Technology Department of Dental MaterialsUniversidade Estadual Paulista - Unesp Institute of Science and Technology Department of Restorative DentistryUniversidade Estadual Paulista (Unesp)Universidade Federal de Uberlândia (UFU)FLRode, Sigmar de Mello [UNESP]Sato, Tabata do Prado [UNESP]Matos, Felipe de SouzaCorreia, Ayla Macyelle de Oliveira [UNESP]Camargo, Samira Esteves Afonso2021-06-25T11:12:32Z2021-06-25T11:12:32Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee025http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0025Brazilian oral research, v. 35, p. e025-.1807-3107http://hdl.handle.net/11449/20846310.1590/1807-3107bor-2021.vol35.00252-s2.0-85101778124Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian oral researchinfo:eu-repo/semantics/openAccess2021-10-23T19:02:12Zoai:repositorio.unesp.br:11449/208463Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:02:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toxicity and effect of whitening toothpastes on enamel surface |
title |
Toxicity and effect of whitening toothpastes on enamel surface |
spellingShingle |
Toxicity and effect of whitening toothpastes on enamel surface Rode, Sigmar de Mello [UNESP] |
title_short |
Toxicity and effect of whitening toothpastes on enamel surface |
title_full |
Toxicity and effect of whitening toothpastes on enamel surface |
title_fullStr |
Toxicity and effect of whitening toothpastes on enamel surface |
title_full_unstemmed |
Toxicity and effect of whitening toothpastes on enamel surface |
title_sort |
Toxicity and effect of whitening toothpastes on enamel surface |
author |
Rode, Sigmar de Mello [UNESP] |
author_facet |
Rode, Sigmar de Mello [UNESP] Sato, Tabata do Prado [UNESP] Matos, Felipe de Souza Correia, Ayla Macyelle de Oliveira [UNESP] Camargo, Samira Esteves Afonso |
author_role |
author |
author2 |
Sato, Tabata do Prado [UNESP] Matos, Felipe de Souza Correia, Ayla Macyelle de Oliveira [UNESP] Camargo, Samira Esteves Afonso |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Uberlândia (UFU) FL |
dc.contributor.author.fl_str_mv |
Rode, Sigmar de Mello [UNESP] Sato, Tabata do Prado [UNESP] Matos, Felipe de Souza Correia, Ayla Macyelle de Oliveira [UNESP] Camargo, Samira Esteves Afonso |
description |
This in vitro study evaluated the biocompatibility and abrasivity of whitening and conventional toothpastes. Samples of conventional (non-whitening) - Edel White Infant (EWI) - and whitening toothpastes - Edel White Whitening (EWW), Edel White CAREFORTE (EWC), Colgate Total 12 Ò Professional (C), and Oral-B Whitening (OB) - were dissolved in culture medium (0.2 g sample weight per mL). Human gingival fibroblasts (hGF) were placed in contact with different dilutions of culture media that had been previously exposed to these toothpastes. Cytotoxicity was then assessed using the methyl tetrazolium test (MTT) and the cell survival rate was determined. Genotoxicity was assessed by the micronucleus test (MNT) and the number of micronuclei was determined before and after exposure to the toothpaste solutions. The enamel surface roughness was evaluated in specimens of bovine teeth (n = 10 per group) before and after 10,000 brushing cycles, using the investigated toothpastes. The results were statistically analyzed using the Mann-Whitney U test and two-way ANOVA (p < 0.05). According to the MTT assay, EWW and OB presented significant cytotoxicity (p < 0.01), but no genotoxic (MNT) effects (p > 0.05). C toothpaste was statistically significantly abrasive to the enamel surface (p < 0.01). The findings of this study may be helpful for individualized selection of commercial toothpastes, as some whitening toothpastes present significant cytotoxicity and conventional toothpaste cause significant surface changes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:12:32Z 2021-06-25T11:12:32Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0025 Brazilian oral research, v. 35, p. e025-. 1807-3107 http://hdl.handle.net/11449/208463 10.1590/1807-3107bor-2021.vol35.0025 2-s2.0-85101778124 |
url |
http://dx.doi.org/10.1590/1807-3107bor-2021.vol35.0025 http://hdl.handle.net/11449/208463 |
identifier_str_mv |
Brazilian oral research, v. 35, p. e025-. 1807-3107 10.1590/1807-3107bor-2021.vol35.0025 2-s2.0-85101778124 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian oral research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e025 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789825093337088 |