Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12011-019-01765-9 http://hdl.handle.net/11449/190391 |
Resumo: | The development of metallomics techniques has allowed for metallomics analysis of biological systems, enabling a better understanding of the response mechanisms for different stimuli, their relationship to metallic species, and the characterization of biomarkers. In this study, a metallomics analysis of the muscle tissue of Nile tilapia was used to aid the understanding of the molecular mechanisms involved in zinc absorption in this fish species when fed organic and/or inorganic sources of zinc and to identify possible biomarkers for the absorption of this micromineral. To accomplish this, the fish were separated into three groups of 24 g, 74 g, and 85 g initial weights, and each group, respectively, was fed a zinc-free diet (control group, G1), a diet containing zinc found in organic sources (treatment 1, G2), and a diet containing zinc from an inorganic source (treatment 2, G3). Two-dimensional polyacrylamide (2D PAGE) gel electrophoresis was used to separate the proteins of the muscle tissue. Subsequently, the expression profiles of protein spots in the samples where zinc was applied in different concentrations were compared, using the software ImageMaster 2D Platinum version 7.0, to identify proteins that were differentially expressed. The identified proteins were then exposed to atomic absorption spectrometry in a graphite furnace to determine zinc mapping and were subsequently characterized via electrospray ionization tandem mass spectrometry (ESI-MS/MS). The metallomic analysis identified 15 proteins differentially expressed and associated with zinc, leading to the conclusion that three metal-binding proteins presented as possible biomarkers of zinc absorption in fish. |
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Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics AnalysisBiomarkerExpressionFishMetallomicsProteinThe development of metallomics techniques has allowed for metallomics analysis of biological systems, enabling a better understanding of the response mechanisms for different stimuli, their relationship to metallic species, and the characterization of biomarkers. In this study, a metallomics analysis of the muscle tissue of Nile tilapia was used to aid the understanding of the molecular mechanisms involved in zinc absorption in this fish species when fed organic and/or inorganic sources of zinc and to identify possible biomarkers for the absorption of this micromineral. To accomplish this, the fish were separated into three groups of 24 g, 74 g, and 85 g initial weights, and each group, respectively, was fed a zinc-free diet (control group, G1), a diet containing zinc found in organic sources (treatment 1, G2), and a diet containing zinc from an inorganic source (treatment 2, G3). Two-dimensional polyacrylamide (2D PAGE) gel electrophoresis was used to separate the proteins of the muscle tissue. Subsequently, the expression profiles of protein spots in the samples where zinc was applied in different concentrations were compared, using the software ImageMaster 2D Platinum version 7.0, to identify proteins that were differentially expressed. The identified proteins were then exposed to atomic absorption spectrometry in a graphite furnace to determine zinc mapping and were subsequently characterized via electrospray ionization tandem mass spectrometry (ESI-MS/MS). The metallomic analysis identified 15 proteins differentially expressed and associated with zinc, leading to the conclusion that three metal-binding proteins presented as possible biomarkers of zinc absorption in fish.School of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)Federal University of Mato Grosso do Sul (UFMS)Biochemistry Department São Paulo University (USP)Biochemistry Department University of Nebraska-UNLSchool of Veterinary Medicine and Animal Science São Paulo State University (UNESP)Institute of Biosciences São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade de São Paulo (USP)University of Nebraska-UNLde Lima, Paula Monteiro [UNESP]Vieira, José Cavalcante Souza [UNESP]Cavecci-Mendonça, Bruna [UNESP]Fleuri, Luciana Francisco [UNESP]de Lima Leite, AlineBuzalaf, Marília Afonso RabeloPezzato, Luiz Edivaldo [UNESP]Braga, Camila Pereirade Magalhães Padilha, Pedro [UNESP]2019-10-06T17:11:40Z2019-10-06T17:11:40Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s12011-019-01765-9Biological Trace Element Research.1559-07200163-4984http://hdl.handle.net/11449/19039110.1007/s12011-019-01765-92-s2.0-8506723022164951487470496889309759030087536Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Trace Element Researchinfo:eu-repo/semantics/openAccess2021-10-22T22:17:21Zoai:repositorio.unesp.br:11449/190391Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T22:17:21Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis |
title |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis |
spellingShingle |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis de Lima, Paula Monteiro [UNESP] Biomarker Expression Fish Metallomics Protein |
title_short |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis |
title_full |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis |
title_fullStr |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis |
title_full_unstemmed |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis |
title_sort |
Identification of Zinc Absorption Biomarkers in Muscle Tissue of Nile Tilapia Fed with Organic and Inorganic Sources of Zinc Using Metallomics Analysis |
author |
de Lima, Paula Monteiro [UNESP] |
author_facet |
de Lima, Paula Monteiro [UNESP] Vieira, José Cavalcante Souza [UNESP] Cavecci-Mendonça, Bruna [UNESP] Fleuri, Luciana Francisco [UNESP] de Lima Leite, Aline Buzalaf, Marília Afonso Rabelo Pezzato, Luiz Edivaldo [UNESP] Braga, Camila Pereira de Magalhães Padilha, Pedro [UNESP] |
author_role |
author |
author2 |
Vieira, José Cavalcante Souza [UNESP] Cavecci-Mendonça, Bruna [UNESP] Fleuri, Luciana Francisco [UNESP] de Lima Leite, Aline Buzalaf, Marília Afonso Rabelo Pezzato, Luiz Edivaldo [UNESP] Braga, Camila Pereira de Magalhães Padilha, Pedro [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade de São Paulo (USP) University of Nebraska-UNL |
dc.contributor.author.fl_str_mv |
de Lima, Paula Monteiro [UNESP] Vieira, José Cavalcante Souza [UNESP] Cavecci-Mendonça, Bruna [UNESP] Fleuri, Luciana Francisco [UNESP] de Lima Leite, Aline Buzalaf, Marília Afonso Rabelo Pezzato, Luiz Edivaldo [UNESP] Braga, Camila Pereira de Magalhães Padilha, Pedro [UNESP] |
dc.subject.por.fl_str_mv |
Biomarker Expression Fish Metallomics Protein |
topic |
Biomarker Expression Fish Metallomics Protein |
description |
The development of metallomics techniques has allowed for metallomics analysis of biological systems, enabling a better understanding of the response mechanisms for different stimuli, their relationship to metallic species, and the characterization of biomarkers. In this study, a metallomics analysis of the muscle tissue of Nile tilapia was used to aid the understanding of the molecular mechanisms involved in zinc absorption in this fish species when fed organic and/or inorganic sources of zinc and to identify possible biomarkers for the absorption of this micromineral. To accomplish this, the fish were separated into three groups of 24 g, 74 g, and 85 g initial weights, and each group, respectively, was fed a zinc-free diet (control group, G1), a diet containing zinc found in organic sources (treatment 1, G2), and a diet containing zinc from an inorganic source (treatment 2, G3). Two-dimensional polyacrylamide (2D PAGE) gel electrophoresis was used to separate the proteins of the muscle tissue. Subsequently, the expression profiles of protein spots in the samples where zinc was applied in different concentrations were compared, using the software ImageMaster 2D Platinum version 7.0, to identify proteins that were differentially expressed. The identified proteins were then exposed to atomic absorption spectrometry in a graphite furnace to determine zinc mapping and were subsequently characterized via electrospray ionization tandem mass spectrometry (ESI-MS/MS). The metallomic analysis identified 15 proteins differentially expressed and associated with zinc, leading to the conclusion that three metal-binding proteins presented as possible biomarkers of zinc absorption in fish. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:11:40Z 2019-10-06T17:11:40Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12011-019-01765-9 Biological Trace Element Research. 1559-0720 0163-4984 http://hdl.handle.net/11449/190391 10.1007/s12011-019-01765-9 2-s2.0-85067230221 6495148747049688 9309759030087536 |
url |
http://dx.doi.org/10.1007/s12011-019-01765-9 http://hdl.handle.net/11449/190391 |
identifier_str_mv |
Biological Trace Element Research. 1559-0720 0163-4984 10.1007/s12011-019-01765-9 2-s2.0-85067230221 6495148747049688 9309759030087536 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Trace Element Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964615931592704 |