Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae)
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-022-18559-0 http://hdl.handle.net/11449/242190 |
Resumo: | Bacillus thuringiensis (Bt) isolates native to Maranhão (BtMA) that are highly toxic to Aedes aegypti larvae and seven standard subspecies of Bt were analyzed for genetic diversity using the rep-PRC technique with BOX, ERIC, REP, MB1, and GTG5 markers. The rep-PCR technique is considered an extremely reliable, reproducible, fast and highly discriminatory technique that may be used even among populations of the same species. These five markers revealed a total of 38 polymorphic DNA fragments for 30 BtMA isolates. Eight groups were obtained with the dendrogram generated through Pearson's correlation analysis, with four groups formed only with BtMA isolates and four comprised of isolates of BtMA and the standard subspecies toxic to dipterans and lepidopterans. Despite the high genetic diversity of BtMA, a low correlation between the collection site, gene content and mortality against A. aegypti larvae was evidenced. The clustering of the standard subspecies of Bt that were toxic against dipterans with BtMA isolates confirm the mosquitocidal action of the native isolates from Maranhão, and they can be used as an alternative for A. aegypti control and other insects of medical importance and for the control of agricultural pests. |
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Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae)Bacillus thuringiensis (Bt) isolates native to Maranhão (BtMA) that are highly toxic to Aedes aegypti larvae and seven standard subspecies of Bt were analyzed for genetic diversity using the rep-PRC technique with BOX, ERIC, REP, MB1, and GTG5 markers. The rep-PCR technique is considered an extremely reliable, reproducible, fast and highly discriminatory technique that may be used even among populations of the same species. These five markers revealed a total of 38 polymorphic DNA fragments for 30 BtMA isolates. Eight groups were obtained with the dendrogram generated through Pearson's correlation analysis, with four groups formed only with BtMA isolates and four comprised of isolates of BtMA and the standard subspecies toxic to dipterans and lepidopterans. Despite the high genetic diversity of BtMA, a low correlation between the collection site, gene content and mortality against A. aegypti larvae was evidenced. The clustering of the standard subspecies of Bt that were toxic against dipterans with BtMA isolates confirm the mosquitocidal action of the native isolates from Maranhão, and they can be used as an alternative for A. aegypti control and other insects of medical importance and for the control of agricultural pests.PostGraduate Program in Biodiversity Environment and Health Center for Higher Studies of Caxias (CESC) State University of Maranhão (UEMA), Praça Duque de Caxias, Morro do Alecrim, S/N, MALaboratory of Entomopathogenic Bacteria and Molecular Markers Center for Higher Studies of Caxias (CESC) State University of Maranhão (UEMA), Praça Duque de Caxias, Morro do Alecrim, S/N, MAPostgraduate Program in Animal Science State University of Maranhão (UEMA) University City Paul VI, Avenida Lourenço Vieira da Silva 1000, Jardim São Cristóvão, MAPostgraduate Program in Agronomy (Agricultural Entomology) São Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesDepartment of Agricultural Production Sciences São Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesDepartment of Agronomy (Entomology) Federal Rural University of Pernambuco (UFRPE), PEDepartment of Chemistry and Biology Laboratory of Entomopathogenic Bacteria and Molecular Markers Center for Higher Studies of Caxias State University of Maranhão, Praça Duque de Caxias, Morro do Alecrim, S/N, MAPostgraduate Program in Agronomy (Agricultural Entomology) São Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesDepartment of Agricultural Production Sciences São Paulo State University (UNESP) School of Agricultural and Veterinarian SciencesUniversidade Estadual de Maringá (UEM)Universidade Estadual Paulista (UNESP)Federal Rural University of Pernambuco (UFRPE)State University of Maranhãoda Costa Fernandes, Geyslade Prado Costa, Dalton Kaynnande Oliveira, Nayanne Santosde Sousa, Emanuelle Cristine PereiraMachado, Déborah Heloísa Bittencourt [UNESP]Polanczyk, Ricardo Antonio [UNESP]de Siqueira, Herbert Álvaro Abreuda Silva, Maria Cleoneide2023-03-02T11:50:26Z2023-03-02T11:50:26Z2022-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-022-18559-0Scientific Reports, v. 12, n. 1, 2022.2045-2322http://hdl.handle.net/11449/24219010.1038/s41598-022-18559-02-s2.0-85136465707Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2023-03-02T11:50:26Zoai:repositorio.unesp.br:11449/242190Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T11:50:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
title |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
spellingShingle |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) da Costa Fernandes, Geysla |
title_short |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
title_full |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
title_fullStr |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
title_full_unstemmed |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
title_sort |
Genetic diversity of Brazilian Bacillus thuringiensis isolates with toxicity against Aedes aegypti (Diptera: Culicidae) |
author |
da Costa Fernandes, Geysla |
author_facet |
da Costa Fernandes, Geysla de Prado Costa, Dalton Kaynnan de Oliveira, Nayanne Santos de Sousa, Emanuelle Cristine Pereira Machado, Déborah Heloísa Bittencourt [UNESP] Polanczyk, Ricardo Antonio [UNESP] de Siqueira, Herbert Álvaro Abreu da Silva, Maria Cleoneide |
author_role |
author |
author2 |
de Prado Costa, Dalton Kaynnan de Oliveira, Nayanne Santos de Sousa, Emanuelle Cristine Pereira Machado, Déborah Heloísa Bittencourt [UNESP] Polanczyk, Ricardo Antonio [UNESP] de Siqueira, Herbert Álvaro Abreu da Silva, Maria Cleoneide |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Maringá (UEM) Universidade Estadual Paulista (UNESP) Federal Rural University of Pernambuco (UFRPE) State University of Maranhão |
dc.contributor.author.fl_str_mv |
da Costa Fernandes, Geysla de Prado Costa, Dalton Kaynnan de Oliveira, Nayanne Santos de Sousa, Emanuelle Cristine Pereira Machado, Déborah Heloísa Bittencourt [UNESP] Polanczyk, Ricardo Antonio [UNESP] de Siqueira, Herbert Álvaro Abreu da Silva, Maria Cleoneide |
description |
Bacillus thuringiensis (Bt) isolates native to Maranhão (BtMA) that are highly toxic to Aedes aegypti larvae and seven standard subspecies of Bt were analyzed for genetic diversity using the rep-PRC technique with BOX, ERIC, REP, MB1, and GTG5 markers. The rep-PCR technique is considered an extremely reliable, reproducible, fast and highly discriminatory technique that may be used even among populations of the same species. These five markers revealed a total of 38 polymorphic DNA fragments for 30 BtMA isolates. Eight groups were obtained with the dendrogram generated through Pearson's correlation analysis, with four groups formed only with BtMA isolates and four comprised of isolates of BtMA and the standard subspecies toxic to dipterans and lepidopterans. Despite the high genetic diversity of BtMA, a low correlation between the collection site, gene content and mortality against A. aegypti larvae was evidenced. The clustering of the standard subspecies of Bt that were toxic against dipterans with BtMA isolates confirm the mosquitocidal action of the native isolates from Maranhão, and they can be used as an alternative for A. aegypti control and other insects of medical importance and for the control of agricultural pests. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-12-01 2023-03-02T11:50:26Z 2023-03-02T11:50:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-022-18559-0 Scientific Reports, v. 12, n. 1, 2022. 2045-2322 http://hdl.handle.net/11449/242190 10.1038/s41598-022-18559-0 2-s2.0-85136465707 |
url |
http://dx.doi.org/10.1038/s41598-022-18559-0 http://hdl.handle.net/11449/242190 |
identifier_str_mv |
Scientific Reports, v. 12, n. 1, 2022. 2045-2322 10.1038/s41598-022-18559-0 2-s2.0-85136465707 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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