Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms24065242 http://hdl.handle.net/11449/247133 |
Resumo: | This study aimed to evaluate the density of the dendritic cells (DCs) and macrophages in oral leukoplakia (OL) and proliferative verrucous leukoplakia (PVL) by immunohistochemical analysis. We analysed paraffined tissue samples of PVL (n = 27), OL (n = 20), and inflammatory fibrous hyperplasia (n = 20) as the control group using the immunomarkers for DCs (CD1a, CD207, CD83, CD208 and CD123) and macrophages (CD68, CD163, FXIIIa and CD209). A quantitative analysis of positive cells in the epithelial and subepithelial areas was determined. Our results showed a reduction in CD208+ cells in the subepithelial area of the OL and PVL compared to the control. Additionally, we found a higher density of FXIIIa+ and CD163+ cells in the subepithelial area in PVL compared to the OL and control. Four-way MANOVA revealed a relationship between increased CD123+ cell density in the subepithelial area of “high-risk” samples regardless of disease. Macrophages provide the first line of defence against PVL antigens, suggesting a distinct pattern of innate immune system activation in PVL compared to OL, which may contribute to the complexity and the high rate of malignant transformation in the PVL. |
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Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigensdendritic cellsmacrophagesmalignant transformationoralproliferative verrucous leukoplakiaThis study aimed to evaluate the density of the dendritic cells (DCs) and macrophages in oral leukoplakia (OL) and proliferative verrucous leukoplakia (PVL) by immunohistochemical analysis. We analysed paraffined tissue samples of PVL (n = 27), OL (n = 20), and inflammatory fibrous hyperplasia (n = 20) as the control group using the immunomarkers for DCs (CD1a, CD207, CD83, CD208 and CD123) and macrophages (CD68, CD163, FXIIIa and CD209). A quantitative analysis of positive cells in the epithelial and subepithelial areas was determined. Our results showed a reduction in CD208+ cells in the subepithelial area of the OL and PVL compared to the control. Additionally, we found a higher density of FXIIIa+ and CD163+ cells in the subepithelial area in PVL compared to the OL and control. Four-way MANOVA revealed a relationship between increased CD123+ cell density in the subepithelial area of “high-risk” samples regardless of disease. Macrophages provide the first line of defence against PVL antigens, suggesting a distinct pattern of innate immune system activation in PVL compared to OL, which may contribute to the complexity and the high rate of malignant transformation in the PVL.Oral Medicine Department of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp), SPOral Pathology Department of Stomatology Public Oral and Forensic Dentistry Ribeirão Preto Dental School University of São Paulo (FORP/USP), SPUWA Dental School The University of Western AustraliaOral Medicine Department of Diagnosis and Surgery School of Dentistry São Paulo State University (Unesp), SPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)The University of Western AustraliaPalaçon, Mariana Paravani [UNESP]de Oliveira Barbeiro, Camila [UNESP]Fernandes, Darcy [UNESP]Biancardi, Mariel Ruivo [UNESP]Silveira, Heitor Albergoni [UNESP]Ferrisse, Túlio Morandin [UNESP]León, Jorge EsquicheKujan, OmarBufalino, Andreia [UNESP]2023-07-29T13:07:08Z2023-07-29T13:07:08Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms24065242International Journal of Molecular Sciences, v. 24, n. 6, 2023.1422-00671661-6596http://hdl.handle.net/11449/24713310.3390/ijms240652422-s2.0-85151963821Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2023-07-29T13:07:08Zoai:repositorio.unesp.br:11449/247133Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:07:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens |
title |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens |
spellingShingle |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens Palaçon, Mariana Paravani [UNESP] dendritic cells macrophages malignant transformation oral proliferative verrucous leukoplakia |
title_short |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens |
title_full |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens |
title_fullStr |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens |
title_full_unstemmed |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens |
title_sort |
Macrophages CD163+ and Factor XIIIa+ Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens |
author |
Palaçon, Mariana Paravani [UNESP] |
author_facet |
Palaçon, Mariana Paravani [UNESP] de Oliveira Barbeiro, Camila [UNESP] Fernandes, Darcy [UNESP] Biancardi, Mariel Ruivo [UNESP] Silveira, Heitor Albergoni [UNESP] Ferrisse, Túlio Morandin [UNESP] León, Jorge Esquiche Kujan, Omar Bufalino, Andreia [UNESP] |
author_role |
author |
author2 |
de Oliveira Barbeiro, Camila [UNESP] Fernandes, Darcy [UNESP] Biancardi, Mariel Ruivo [UNESP] Silveira, Heitor Albergoni [UNESP] Ferrisse, Túlio Morandin [UNESP] León, Jorge Esquiche Kujan, Omar Bufalino, Andreia [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) The University of Western Australia |
dc.contributor.author.fl_str_mv |
Palaçon, Mariana Paravani [UNESP] de Oliveira Barbeiro, Camila [UNESP] Fernandes, Darcy [UNESP] Biancardi, Mariel Ruivo [UNESP] Silveira, Heitor Albergoni [UNESP] Ferrisse, Túlio Morandin [UNESP] León, Jorge Esquiche Kujan, Omar Bufalino, Andreia [UNESP] |
dc.subject.por.fl_str_mv |
dendritic cells macrophages malignant transformation oral proliferative verrucous leukoplakia |
topic |
dendritic cells macrophages malignant transformation oral proliferative verrucous leukoplakia |
description |
This study aimed to evaluate the density of the dendritic cells (DCs) and macrophages in oral leukoplakia (OL) and proliferative verrucous leukoplakia (PVL) by immunohistochemical analysis. We analysed paraffined tissue samples of PVL (n = 27), OL (n = 20), and inflammatory fibrous hyperplasia (n = 20) as the control group using the immunomarkers for DCs (CD1a, CD207, CD83, CD208 and CD123) and macrophages (CD68, CD163, FXIIIa and CD209). A quantitative analysis of positive cells in the epithelial and subepithelial areas was determined. Our results showed a reduction in CD208+ cells in the subepithelial area of the OL and PVL compared to the control. Additionally, we found a higher density of FXIIIa+ and CD163+ cells in the subepithelial area in PVL compared to the OL and control. Four-way MANOVA revealed a relationship between increased CD123+ cell density in the subepithelial area of “high-risk” samples regardless of disease. Macrophages provide the first line of defence against PVL antigens, suggesting a distinct pattern of innate immune system activation in PVL compared to OL, which may contribute to the complexity and the high rate of malignant transformation in the PVL. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:07:08Z 2023-07-29T13:07:08Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms24065242 International Journal of Molecular Sciences, v. 24, n. 6, 2023. 1422-0067 1661-6596 http://hdl.handle.net/11449/247133 10.3390/ijms24065242 2-s2.0-85151963821 |
url |
http://dx.doi.org/10.3390/ijms24065242 http://hdl.handle.net/11449/247133 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 24, n. 6, 2023. 1422-0067 1661-6596 10.3390/ijms24065242 2-s2.0-85151963821 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964836807835648 |