An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.cbpa.2015.06.012 http://hdl.handle.net/11449/160766 |
Resumo: | Measurement of heart rate (f(H)) in embryonic reptiles has previously imposed some degree of invasive treatment on the developing embryo. Recently a non-invasive technique of f(H) detection from intact eggs was developed for commercial avian breeders and has since been used in biological research. This device uses infrared light, enabling it to detect heartbeats in very early embryos. However, infrared light is a source of heat and extended enclosure of an egg in the device is likely to affect temperature with consequent effects on physiological processes, including f(H). We studied the effect of use of the monitor on the temperature of eggs and on fH in two species of reptiles, the snapping turtle (Chelydra serpentina) and the green iguana (Iguana iguana). Egg temperature increased from a room temperature of 27-28 degrees C, by 26% in turtles and 14% in iguanas over 1 h of enclosure, resulting in an increase in f(H) of 76-81% in turtles and 35-50% iguanas. These effects on f(H) can either be avoided by brief enclosure of each egg in the monitor or measured and accounted for during the design of long-term experiments. (C) 2015 Elsevier Inc. All rights reserved. |
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An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryosReptilesEmbryonic developmentHeart rateBuddy (R)Infrared radiationTemperatureMeasurement of heart rate (f(H)) in embryonic reptiles has previously imposed some degree of invasive treatment on the developing embryo. Recently a non-invasive technique of f(H) detection from intact eggs was developed for commercial avian breeders and has since been used in biological research. This device uses infrared light, enabling it to detect heartbeats in very early embryos. However, infrared light is a source of heat and extended enclosure of an egg in the device is likely to affect temperature with consequent effects on physiological processes, including f(H). We studied the effect of use of the monitor on the temperature of eggs and on fH in two species of reptiles, the snapping turtle (Chelydra serpentina) and the green iguana (Iguana iguana). Egg temperature increased from a room temperature of 27-28 degrees C, by 26% in turtles and 14% in iguanas over 1 h of enclosure, resulting in an increase in f(H) of 76-81% in turtles and 35-50% iguanas. These effects on f(H) can either be avoided by brief enclosure of each egg in the monitor or measured and accounted for during the design of long-term experiments. (C) 2015 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Science FoundationUniv Estadual Paulista, Inst Biociencias, Dept Zool, Rio Claro, SP, BrazilUniv Birmingham, Sch Biosci, Birmingham B15 2TT, W Midlands, EnglandUniv N Texas, Dept Biol Sci, Dev Integrat Biol Cluster, Denton, TX 76203 USAUniv Estadual Paulista, Inst Biociencias, Dept Zool, Rio Claro, SP, BrazilFAPESP: 2012/06938-8FAPESP: 2012/16537-0National Science Foundation: IOS-0845741Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ BirminghamUniv N TexasSartori, Marina R. [UNESP]Taylor, Edwin W. [UNESP]Abe, Augusto S. [UNESP]Crossley, Dane A.2018-11-26T16:16:38Z2018-11-26T16:16:38Z2015-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-21application/pdfhttp://dx.doi.org/10.1016/j.cbpa.2015.06.012Comparative Biochemistry And Physiology A-molecular & Integrative Physiology. New York: Elsevier Science Inc, v. 188, p. 17-21, 2015.1095-6433http://hdl.handle.net/11449/16076610.1016/j.cbpa.2015.06.012WOS:000360513400003WOS000360513400003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengComparative Biochemistry And Physiology A-molecular & Integrative Physiology0,836info:eu-repo/semantics/openAccess2023-10-29T06:05:34Zoai:repositorio.unesp.br:11449/160766Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-29T06:05:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos |
title |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos |
spellingShingle |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos Sartori, Marina R. [UNESP] Reptiles Embryonic development Heart rate Buddy (R) Infrared radiation Temperature |
title_short |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos |
title_full |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos |
title_fullStr |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos |
title_full_unstemmed |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos |
title_sort |
An appraisal of the use of an infrared digital monitoring system for long-term measurement of heart rate in reptilian embryos |
author |
Sartori, Marina R. [UNESP] |
author_facet |
Sartori, Marina R. [UNESP] Taylor, Edwin W. [UNESP] Abe, Augusto S. [UNESP] Crossley, Dane A. |
author_role |
author |
author2 |
Taylor, Edwin W. [UNESP] Abe, Augusto S. [UNESP] Crossley, Dane A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Birmingham Univ N Texas |
dc.contributor.author.fl_str_mv |
Sartori, Marina R. [UNESP] Taylor, Edwin W. [UNESP] Abe, Augusto S. [UNESP] Crossley, Dane A. |
dc.subject.por.fl_str_mv |
Reptiles Embryonic development Heart rate Buddy (R) Infrared radiation Temperature |
topic |
Reptiles Embryonic development Heart rate Buddy (R) Infrared radiation Temperature |
description |
Measurement of heart rate (f(H)) in embryonic reptiles has previously imposed some degree of invasive treatment on the developing embryo. Recently a non-invasive technique of f(H) detection from intact eggs was developed for commercial avian breeders and has since been used in biological research. This device uses infrared light, enabling it to detect heartbeats in very early embryos. However, infrared light is a source of heat and extended enclosure of an egg in the device is likely to affect temperature with consequent effects on physiological processes, including f(H). We studied the effect of use of the monitor on the temperature of eggs and on fH in two species of reptiles, the snapping turtle (Chelydra serpentina) and the green iguana (Iguana iguana). Egg temperature increased from a room temperature of 27-28 degrees C, by 26% in turtles and 14% in iguanas over 1 h of enclosure, resulting in an increase in f(H) of 76-81% in turtles and 35-50% iguanas. These effects on f(H) can either be avoided by brief enclosure of each egg in the monitor or measured and accounted for during the design of long-term experiments. (C) 2015 Elsevier Inc. All rights reserved. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 2018-11-26T16:16:38Z 2018-11-26T16:16:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.cbpa.2015.06.012 Comparative Biochemistry And Physiology A-molecular & Integrative Physiology. New York: Elsevier Science Inc, v. 188, p. 17-21, 2015. 1095-6433 http://hdl.handle.net/11449/160766 10.1016/j.cbpa.2015.06.012 WOS:000360513400003 WOS000360513400003.pdf |
url |
http://dx.doi.org/10.1016/j.cbpa.2015.06.012 http://hdl.handle.net/11449/160766 |
identifier_str_mv |
Comparative Biochemistry And Physiology A-molecular & Integrative Physiology. New York: Elsevier Science Inc, v. 188, p. 17-21, 2015. 1095-6433 10.1016/j.cbpa.2015.06.012 WOS:000360513400003 WOS000360513400003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Comparative Biochemistry And Physiology A-molecular & Integrative Physiology 0,836 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-21 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964740151148544 |