Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0103-6440201902241 http://hdl.handle.net/11449/189018 |
Resumo: | In the present study we compared the effects of the selective COX-2 inhibitor etoricoxib with those of the classical non-selective NSAID diclofenac on the inflammatory process and alveolar bone loss in an experimental model of periodontitis in rats. Ninety male Holtzman rats (250 g) were randomly sorted into four experimental groups: Sham+CMC and Ligature+CMC (control) groups which received 0.5% carboxymethylcellulose sodium (CMC) solution; Ligature+Diclofenac and Ligature+Etoricoxib groups which received Potassium Diclofenac and Etoricoxib, respectively, suspended in 0.5% CMC (10 mg/kg/day). At 7, 14 and 21 days after placing ligatures in the cervical region of both the lower right and left first molars, the animals were euthanized. At the end of each period, the mandibles were collected for radiographic examination of alveolar bone loss. In addition, alveolar bone and periodontal ligament tissue samples were collected for COX-2 expression analysis and gingival tissues were collected for measurement of PGE2 contents. Animals with ligature-induced periodontal disease showed significant increased COX-2 gene expression at days 7, 14 and 21 (p<0.05) on alveolar bone and periodontal ligament. However, both treatments resulted in significantly reduced alveolar bone loss when compared to the untreated Ligature group (p<0.05), with no statistical difference between Etoricoxib and Diclofenac Potassium groups. This study shows that both drugs were able to reduce alveolar bone loss after periodontal disease induction. |
id |
UNSP_cdbc47264bfaa0aa84c2a8947ada214a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/189018 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitisCyclooxygenase inhibitorsPeriodontitisRatsIn the present study we compared the effects of the selective COX-2 inhibitor etoricoxib with those of the classical non-selective NSAID diclofenac on the inflammatory process and alveolar bone loss in an experimental model of periodontitis in rats. Ninety male Holtzman rats (250 g) were randomly sorted into four experimental groups: Sham+CMC and Ligature+CMC (control) groups which received 0.5% carboxymethylcellulose sodium (CMC) solution; Ligature+Diclofenac and Ligature+Etoricoxib groups which received Potassium Diclofenac and Etoricoxib, respectively, suspended in 0.5% CMC (10 mg/kg/day). At 7, 14 and 21 days after placing ligatures in the cervical region of both the lower right and left first molars, the animals were euthanized. At the end of each period, the mandibles were collected for radiographic examination of alveolar bone loss. In addition, alveolar bone and periodontal ligament tissue samples were collected for COX-2 expression analysis and gingival tissues were collected for measurement of PGE2 contents. Animals with ligature-induced periodontal disease showed significant increased COX-2 gene expression at days 7, 14 and 21 (p<0.05) on alveolar bone and periodontal ligament. However, both treatments resulted in significantly reduced alveolar bone loss when compared to the untreated Ligature group (p<0.05), with no statistical difference between Etoricoxib and Diclofenac Potassium groups. This study shows that both drugs were able to reduce alveolar bone loss after periodontal disease induction.Department of Stomatology Discipline of Periodontology School of Dentistry USP – Universidade de São PauloDepartment of Pharmacology Institute of Biomedical Sciences USP – Universidade de São PauloDepartment of Oral Pathology Dental School of Araraquara UNESP – Universidade Estadual PaulistaDepartment of Oral Pathology Dental School of Araraquara UNESP – Universidade Estadual PaulistaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Moro, Marcella GoetzOliveira, Marilia Dantas Dos SantosOliveira, Leticia Rodrigues DeTeixeira, Simone AparecidaMuscará, Marcelo NicolasSpolidorio, Luis Carlos [UNESP]Holzhausen, Marinella2019-10-06T16:27:12Z2019-10-06T16:27:12Z2019-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article133-138application/pdfhttp://dx.doi.org/10.1590/0103-6440201902241Brazilian Dental Journal, v. 30, n. 2, p. 133-138, 2019.1806-47600103-6440http://hdl.handle.net/11449/18901810.1590/0103-6440201902241S0103-644020190002001332-s2.0-85064721662S0103-64402019000200133.pdf2640929291808415Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Dental Journalinfo:eu-repo/semantics/openAccess2023-12-24T06:19:49Zoai:repositorio.unesp.br:11449/189018Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-24T06:19:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis |
title |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis |
spellingShingle |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis Moro, Marcella Goetz Cyclooxygenase inhibitors Periodontitis Rats |
title_short |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis |
title_full |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis |
title_fullStr |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis |
title_full_unstemmed |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis |
title_sort |
Effects of selective versus non- selective cox-2 inhibition on experimental periodontitis |
author |
Moro, Marcella Goetz |
author_facet |
Moro, Marcella Goetz Oliveira, Marilia Dantas Dos Santos Oliveira, Leticia Rodrigues De Teixeira, Simone Aparecida Muscará, Marcelo Nicolas Spolidorio, Luis Carlos [UNESP] Holzhausen, Marinella |
author_role |
author |
author2 |
Oliveira, Marilia Dantas Dos Santos Oliveira, Leticia Rodrigues De Teixeira, Simone Aparecida Muscará, Marcelo Nicolas Spolidorio, Luis Carlos [UNESP] Holzhausen, Marinella |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Moro, Marcella Goetz Oliveira, Marilia Dantas Dos Santos Oliveira, Leticia Rodrigues De Teixeira, Simone Aparecida Muscará, Marcelo Nicolas Spolidorio, Luis Carlos [UNESP] Holzhausen, Marinella |
dc.subject.por.fl_str_mv |
Cyclooxygenase inhibitors Periodontitis Rats |
topic |
Cyclooxygenase inhibitors Periodontitis Rats |
description |
In the present study we compared the effects of the selective COX-2 inhibitor etoricoxib with those of the classical non-selective NSAID diclofenac on the inflammatory process and alveolar bone loss in an experimental model of periodontitis in rats. Ninety male Holtzman rats (250 g) were randomly sorted into four experimental groups: Sham+CMC and Ligature+CMC (control) groups which received 0.5% carboxymethylcellulose sodium (CMC) solution; Ligature+Diclofenac and Ligature+Etoricoxib groups which received Potassium Diclofenac and Etoricoxib, respectively, suspended in 0.5% CMC (10 mg/kg/day). At 7, 14 and 21 days after placing ligatures in the cervical region of both the lower right and left first molars, the animals were euthanized. At the end of each period, the mandibles were collected for radiographic examination of alveolar bone loss. In addition, alveolar bone and periodontal ligament tissue samples were collected for COX-2 expression analysis and gingival tissues were collected for measurement of PGE2 contents. Animals with ligature-induced periodontal disease showed significant increased COX-2 gene expression at days 7, 14 and 21 (p<0.05) on alveolar bone and periodontal ligament. However, both treatments resulted in significantly reduced alveolar bone loss when compared to the untreated Ligature group (p<0.05), with no statistical difference between Etoricoxib and Diclofenac Potassium groups. This study shows that both drugs were able to reduce alveolar bone loss after periodontal disease induction. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:27:12Z 2019-10-06T16:27:12Z 2019-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0103-6440201902241 Brazilian Dental Journal, v. 30, n. 2, p. 133-138, 2019. 1806-4760 0103-6440 http://hdl.handle.net/11449/189018 10.1590/0103-6440201902241 S0103-64402019000200133 2-s2.0-85064721662 S0103-64402019000200133.pdf 2640929291808415 |
url |
http://dx.doi.org/10.1590/0103-6440201902241 http://hdl.handle.net/11449/189018 |
identifier_str_mv |
Brazilian Dental Journal, v. 30, n. 2, p. 133-138, 2019. 1806-4760 0103-6440 10.1590/0103-6440201902241 S0103-64402019000200133 2-s2.0-85064721662 S0103-64402019000200133.pdf 2640929291808415 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Dental Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
133-138 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965394165825536 |