Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products
Main Author: | |
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Publication Date: | 2007 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/S1415-47572007000400023 http://hdl.handle.net/11449/30496 |
Summary: | In this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric region of all chromosomes, with the exception of the y p. Silver nitrate staining revealed one nucleolus organizer region (NOR) on the terminal region of the short arm of the second autosome pair. Silver staining of meiotic cells confirmed the NOR pattern detected in mitotic cells and revealed an argentophilous material on the Xy p. A cytogenetic comparison between the two populations of A. variegatus showed a statistically significant divergence (chi2 = 117.10; df = 1) in the number of aneuploid cells and a higher frequency of B chromosome in the population exposed to agrochemicals. |
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Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical productsB chromosomeconstitutive heterochromatinkaryotypemeiosisAg-NORsIn this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric region of all chromosomes, with the exception of the y p. Silver nitrate staining revealed one nucleolus organizer region (NOR) on the terminal region of the short arm of the second autosome pair. Silver staining of meiotic cells confirmed the NOR pattern detected in mitotic cells and revealed an argentophilous material on the Xy p. A cytogenetic comparison between the two populations of A. variegatus showed a statistically significant divergence (chi2 = 117.10; df = 1) in the number of aneuploid cells and a higher frequency of B chromosome in the population exposed to agrochemicals.Universidade Estadual Paulista Instituto de Biociências Departamento de BiologiaUniversidade Estadual de Ponta Grossa Departamento de Biologia Estrutural, Molecular e GenéticaUniversidade Estadual de Ponta Grossa Departamento de Fitotecnia e FitossanidadeUniversidade Estadual Paulista Instituto de Biociências Departamento de BiologiaSociedade Brasileira de GenéticaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Ponta Grossa (UEPG)Schneider, Marielle Cristina [UNESP]Carraro, Bianca PierinaCella, Doralice Maria [UNESP]Matiello, Rodrigo RodriguesArtoni, Roberto FerreiraAlmeida, Mara Cristina2014-05-20T15:17:30Z2014-05-20T15:17:30Z2007-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article640-645application/pdfhttp://dx.doi.org/10.1590/S1415-47572007000400023Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 640-645, 2007.1415-4757http://hdl.handle.net/11449/3049610.1590/S1415-47572007000400023S1415-47572007000400023S1415-47572007000400023.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-10-15T06:03:55Zoai:repositorio.unesp.br:11449/30496Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-15T06:03:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products |
title |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products |
spellingShingle |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products Schneider, Marielle Cristina [UNESP] B chromosome constitutive heterochromatin karyotype meiosis Ag-NORs |
title_short |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products |
title_full |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products |
title_fullStr |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products |
title_full_unstemmed |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products |
title_sort |
Astylus variegatus (Coleoptera, Melyridae): Cytogenetic study of a population exposed to agrochemical products |
author |
Schneider, Marielle Cristina [UNESP] |
author_facet |
Schneider, Marielle Cristina [UNESP] Carraro, Bianca Pierina Cella, Doralice Maria [UNESP] Matiello, Rodrigo Rodrigues Artoni, Roberto Ferreira Almeida, Mara Cristina |
author_role |
author |
author2 |
Carraro, Bianca Pierina Cella, Doralice Maria [UNESP] Matiello, Rodrigo Rodrigues Artoni, Roberto Ferreira Almeida, Mara Cristina |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Schneider, Marielle Cristina [UNESP] Carraro, Bianca Pierina Cella, Doralice Maria [UNESP] Matiello, Rodrigo Rodrigues Artoni, Roberto Ferreira Almeida, Mara Cristina |
dc.subject.por.fl_str_mv |
B chromosome constitutive heterochromatin karyotype meiosis Ag-NORs |
topic |
B chromosome constitutive heterochromatin karyotype meiosis Ag-NORs |
description |
In this work we describe the cytogenetic analyses performed in specimens of Astylus variegatus (Germar, 1824) collected in two localities: one area of natural vegetation and one of agricultural crops, where agrochemical products were used. Astylus variegatus had karyotypes with 2n(male) = 16+Xy p and 2n (female) = 16+XXp, with exclusively metacentric chromosomes. Pachytene spermatocytes showed synapsed autosomal bivalents and non-associated sex chromosomes. In diplotene, the autosomal bivalents exhibited one or two terminal chiasmata and the Xy p had a typical parachute configuration. In meiotic cells of some specimens, an extra chromosome, interpreted as a B chromosome, was observed. C-banding showed constitutive heterochromatin in the pericentromeric region of all chromosomes, with the exception of the y p. Silver nitrate staining revealed one nucleolus organizer region (NOR) on the terminal region of the short arm of the second autosome pair. Silver staining of meiotic cells confirmed the NOR pattern detected in mitotic cells and revealed an argentophilous material on the Xy p. A cytogenetic comparison between the two populations of A. variegatus showed a statistically significant divergence (chi2 = 117.10; df = 1) in the number of aneuploid cells and a higher frequency of B chromosome in the population exposed to agrochemicals. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-01-01 2014-05-20T15:17:30Z 2014-05-20T15:17:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572007000400023 Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 640-645, 2007. 1415-4757 http://hdl.handle.net/11449/30496 10.1590/S1415-47572007000400023 S1415-47572007000400023 S1415-47572007000400023.pdf |
url |
http://dx.doi.org/10.1590/S1415-47572007000400023 http://hdl.handle.net/11449/30496 |
identifier_str_mv |
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 30, n. 3, p. 640-645, 2007. 1415-4757 10.1590/S1415-47572007000400023 S1415-47572007000400023 S1415-47572007000400023.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
640-645 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789399192174592 |