Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jdent.2021.103719 http://hdl.handle.net/11449/229080 |
Resumo: | Objectives: To evaluate obliterating capability and biological performance of desensitizing agents. Methods: 50 dentin blocks were distributed according to the desensitizing agent used (n = 10): Control (Artificial saliva); Ultra EZ (Ultradent); Desensibilize Nano P (FGM); T5-OH Bioactive Glass (Experimental solution); F18 Bioactive Glass (Experimental solution). Desensitizing treatments were performed for 15 days. In addition, specimens were subjected to acid challenge to simulate oral environment demineralizing conditions. Samples were subjected to permeability analysis before and after desensitizing procedures and acid challenge. Cytotoxicity analysis was performed by using Alamar Blue assay and complemented by total protein quantification by Pierce Bicinchoninic Acid assay at 15 min, 24-h and 48-h time points. Scanning electron microscopy and energy dispersion X-ray spectroscopy were performed for qualitative analysis. Data of dentin permeability was analyzed by two-way repeated measures ANOVA and Tukey's test. For cytotoxicity, Kruskal-Wallis and Newman-Keuls tests. Results: for dentin permeability there was no significant difference among desensitizing agents after treatment, but control group presented highest values (0.131 ± 0.076 Lp). After acid challenge, control group maintained highest values (0.044 ± 0.014 Lp) with significant difference to other groups, except for Desensibilize Nano P (0.037 ± 0.019 Lp). For cytotoxicity, there were no significant differences among groups. Conclusion: Bioglass-based desensitizers caused similar effects to commercially available products, regarding permeability and dentin biological properties. Clinical significance: There is no gold standard protocol for dentin sensitivity. The study of novel desensitizing agents that can obliterate dentinal tubules in a faster-acting and long-lasting way may help meet this clinical need. |
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Analysis of permeability and biological properties of dentin treated with experimental bioactive glassesCell biologyDentin permeabilityDentin sensitivityDesensitizing agentsObjectives: To evaluate obliterating capability and biological performance of desensitizing agents. Methods: 50 dentin blocks were distributed according to the desensitizing agent used (n = 10): Control (Artificial saliva); Ultra EZ (Ultradent); Desensibilize Nano P (FGM); T5-OH Bioactive Glass (Experimental solution); F18 Bioactive Glass (Experimental solution). Desensitizing treatments were performed for 15 days. In addition, specimens were subjected to acid challenge to simulate oral environment demineralizing conditions. Samples were subjected to permeability analysis before and after desensitizing procedures and acid challenge. Cytotoxicity analysis was performed by using Alamar Blue assay and complemented by total protein quantification by Pierce Bicinchoninic Acid assay at 15 min, 24-h and 48-h time points. Scanning electron microscopy and energy dispersion X-ray spectroscopy were performed for qualitative analysis. Data of dentin permeability was analyzed by two-way repeated measures ANOVA and Tukey's test. For cytotoxicity, Kruskal-Wallis and Newman-Keuls tests. Results: for dentin permeability there was no significant difference among desensitizing agents after treatment, but control group presented highest values (0.131 ± 0.076 Lp). After acid challenge, control group maintained highest values (0.044 ± 0.014 Lp) with significant difference to other groups, except for Desensibilize Nano P (0.037 ± 0.019 Lp). For cytotoxicity, there were no significant differences among groups. Conclusion: Bioglass-based desensitizers caused similar effects to commercially available products, regarding permeability and dentin biological properties. Clinical significance: There is no gold standard protocol for dentin sensitivity. The study of novel desensitizing agents that can obliterate dentinal tubules in a faster-acting and long-lasting way may help meet this clinical need.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, 1193 José Bonifácio St.16015-050 AraçatubaUniversity of Toronto Faculty of Dentistry Department of Restorative Dentistry, RM 537124 Edward St.Federal University of São Carlos (UFSCar) Department of Materials Engineering Vitreous Materials Laboratory (LaMaV) CP. 676, 13565-905Professor São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Dental Materials and Prosthodontics, 1193 José Bonifácio St.16015-050 AraçatubaSão Paulo State University (UNESP) School of Dentistry Araçatuba Department of Preventive and Restorative Dentistry, 1193 José Bonifácio St.16015-050 AraçatubaProfessor São Paulo State University (UNESP) School of Dentistry Araçatuba Department of Dental Materials and Prosthodontics, 1193 José Bonifácio St.16015-050 AraçatubaCAPES: 88887.363216/2019-00Universidade Estadual Paulista (UNESP)Faculty of DentistryUniversidade Federal de São Carlos (UFSCar)Reis, Bruna de Oliveira [UNESP]Prakki, AnuradhaStavroullakis, Alexander TerrySouza, Marina TrevelinSiqueira, Renato LuizZanotto, Edgar DutraBriso, André Luiz Fraga [UNESP]Tavares Ângelo Cintra, Luciano [UNESP]Henrique dos Santos, Paulo [UNESP]2022-04-29T08:30:16Z2022-04-29T08:30:16Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jdent.2021.103719Journal of Dentistry, v. 111.0300-5712http://hdl.handle.net/11449/22908010.1016/j.jdent.2021.1037192-s2.0-85109100077Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Dentistryinfo:eu-repo/semantics/openAccess2022-04-29T08:30:16Zoai:repositorio.unesp.br:11449/229080Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:30:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses |
title |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses |
spellingShingle |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses Reis, Bruna de Oliveira [UNESP] Cell biology Dentin permeability Dentin sensitivity Desensitizing agents |
title_short |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses |
title_full |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses |
title_fullStr |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses |
title_full_unstemmed |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses |
title_sort |
Analysis of permeability and biological properties of dentin treated with experimental bioactive glasses |
author |
Reis, Bruna de Oliveira [UNESP] |
author_facet |
Reis, Bruna de Oliveira [UNESP] Prakki, Anuradha Stavroullakis, Alexander Terry Souza, Marina Trevelin Siqueira, Renato Luiz Zanotto, Edgar Dutra Briso, André Luiz Fraga [UNESP] Tavares Ângelo Cintra, Luciano [UNESP] Henrique dos Santos, Paulo [UNESP] |
author_role |
author |
author2 |
Prakki, Anuradha Stavroullakis, Alexander Terry Souza, Marina Trevelin Siqueira, Renato Luiz Zanotto, Edgar Dutra Briso, André Luiz Fraga [UNESP] Tavares Ângelo Cintra, Luciano [UNESP] Henrique dos Santos, Paulo [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Faculty of Dentistry Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Reis, Bruna de Oliveira [UNESP] Prakki, Anuradha Stavroullakis, Alexander Terry Souza, Marina Trevelin Siqueira, Renato Luiz Zanotto, Edgar Dutra Briso, André Luiz Fraga [UNESP] Tavares Ângelo Cintra, Luciano [UNESP] Henrique dos Santos, Paulo [UNESP] |
dc.subject.por.fl_str_mv |
Cell biology Dentin permeability Dentin sensitivity Desensitizing agents |
topic |
Cell biology Dentin permeability Dentin sensitivity Desensitizing agents |
description |
Objectives: To evaluate obliterating capability and biological performance of desensitizing agents. Methods: 50 dentin blocks were distributed according to the desensitizing agent used (n = 10): Control (Artificial saliva); Ultra EZ (Ultradent); Desensibilize Nano P (FGM); T5-OH Bioactive Glass (Experimental solution); F18 Bioactive Glass (Experimental solution). Desensitizing treatments were performed for 15 days. In addition, specimens were subjected to acid challenge to simulate oral environment demineralizing conditions. Samples were subjected to permeability analysis before and after desensitizing procedures and acid challenge. Cytotoxicity analysis was performed by using Alamar Blue assay and complemented by total protein quantification by Pierce Bicinchoninic Acid assay at 15 min, 24-h and 48-h time points. Scanning electron microscopy and energy dispersion X-ray spectroscopy were performed for qualitative analysis. Data of dentin permeability was analyzed by two-way repeated measures ANOVA and Tukey's test. For cytotoxicity, Kruskal-Wallis and Newman-Keuls tests. Results: for dentin permeability there was no significant difference among desensitizing agents after treatment, but control group presented highest values (0.131 ± 0.076 Lp). After acid challenge, control group maintained highest values (0.044 ± 0.014 Lp) with significant difference to other groups, except for Desensibilize Nano P (0.037 ± 0.019 Lp). For cytotoxicity, there were no significant differences among groups. Conclusion: Bioglass-based desensitizers caused similar effects to commercially available products, regarding permeability and dentin biological properties. Clinical significance: There is no gold standard protocol for dentin sensitivity. The study of novel desensitizing agents that can obliterate dentinal tubules in a faster-acting and long-lasting way may help meet this clinical need. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-04-29T08:30:16Z 2022-04-29T08:30:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jdent.2021.103719 Journal of Dentistry, v. 111. 0300-5712 http://hdl.handle.net/11449/229080 10.1016/j.jdent.2021.103719 2-s2.0-85109100077 |
url |
http://dx.doi.org/10.1016/j.jdent.2021.103719 http://hdl.handle.net/11449/229080 |
identifier_str_mv |
Journal of Dentistry, v. 111. 0300-5712 10.1016/j.jdent.2021.103719 2-s2.0-85109100077 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789576163491840 |