Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections

Detalhes bibliográficos
Autor(a) principal: Maluta, Renato Pariz [UNESP]
Data de Publicação: 2012
Outros Autores: Stella, Ariel Eurides, Riccardi, Kátia [UNESP], Rigobelo, Everlon Cid [UNESP], Marin, José Moacir, Carvalho, Marileda Bonafim [UNESP], Ávila, Fernando Antonio de [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1517-83822012000100045
http://hdl.handle.net/11449/2942
Resumo: Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6:H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E.
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spelling Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infectionsBiomarkersbiotypingEric-PCRUPECPathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6:H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Universidade Estadual PaulistaUniversidade Federal de Goiás (UFG)Universidade de São Paulo Faculdade de Odontologia de Ribeirão PretoUniversidade Estadual PaulistaFUNDUNESP: 01198/08-DFPSociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Universidade Federal de Goiás (UFG)Universidade de São Paulo (USP)Maluta, Renato Pariz [UNESP]Stella, Ariel EuridesRiccardi, Kátia [UNESP]Rigobelo, Everlon Cid [UNESP]Marin, José MoacirCarvalho, Marileda Bonafim [UNESP]Ávila, Fernando Antonio de [UNESP]2014-05-20T13:15:56Z2014-05-20T13:15:56Z2012-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article375-381application/pdfhttp://dx.doi.org/10.1590/S1517-83822012000100045Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 1, p. 375-381, 2012.1517-8382http://hdl.handle.net/11449/294210.1590/S1517-83822012000100045S1517-83822012000100045WOS:000303939800045S1517-83822012000100045.pdf301187271510908107466476017663900000-0002-9779-2213SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-06-07T13:02:37Zoai:repositorio.unesp.br:11449/2942Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:42:29.198198Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
title Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
spellingShingle Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
Maluta, Renato Pariz [UNESP]
Biomarkers
biotyping
Eric-PCR
UPEC
title_short Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
title_full Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
title_fullStr Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
title_full_unstemmed Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
title_sort Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
author Maluta, Renato Pariz [UNESP]
author_facet Maluta, Renato Pariz [UNESP]
Stella, Ariel Eurides
Riccardi, Kátia [UNESP]
Rigobelo, Everlon Cid [UNESP]
Marin, José Moacir
Carvalho, Marileda Bonafim [UNESP]
Ávila, Fernando Antonio de [UNESP]
author_role author
author2 Stella, Ariel Eurides
Riccardi, Kátia [UNESP]
Rigobelo, Everlon Cid [UNESP]
Marin, José Moacir
Carvalho, Marileda Bonafim [UNESP]
Ávila, Fernando Antonio de [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de Goiás (UFG)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Maluta, Renato Pariz [UNESP]
Stella, Ariel Eurides
Riccardi, Kátia [UNESP]
Rigobelo, Everlon Cid [UNESP]
Marin, José Moacir
Carvalho, Marileda Bonafim [UNESP]
Ávila, Fernando Antonio de [UNESP]
dc.subject.por.fl_str_mv Biomarkers
biotyping
Eric-PCR
UPEC
topic Biomarkers
biotyping
Eric-PCR
UPEC
description Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6:H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-01
2014-05-20T13:15:56Z
2014-05-20T13:15:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822012000100045
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 1, p. 375-381, 2012.
1517-8382
http://hdl.handle.net/11449/2942
10.1590/S1517-83822012000100045
S1517-83822012000100045
WOS:000303939800045
S1517-83822012000100045.pdf
3011872715109081
0746647601766390
0000-0002-9779-2213
url http://dx.doi.org/10.1590/S1517-83822012000100045
http://hdl.handle.net/11449/2942
identifier_str_mv Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 1, p. 375-381, 2012.
1517-8382
10.1590/S1517-83822012000100045
S1517-83822012000100045
WOS:000303939800045
S1517-83822012000100045.pdf
3011872715109081
0746647601766390
0000-0002-9779-2213
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Microbiology
1.810
0,630
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 375-381
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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