Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1517-83822012000100045 http://hdl.handle.net/11449/2942 |
Resumo: | Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6:H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E. |
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Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infectionsBiomarkersbiotypingEric-PCRUPECPathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6:H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação para o Desenvolvimento da UNESP (FUNDUNESP)Universidade Estadual PaulistaUniversidade Federal de Goiás (UFG)Universidade de São Paulo Faculdade de Odontologia de Ribeirão PretoUniversidade Estadual PaulistaFUNDUNESP: 01198/08-DFPSociedade Brasileira de MicrobiologiaUniversidade Estadual Paulista (Unesp)Universidade Federal de Goiás (UFG)Universidade de São Paulo (USP)Maluta, Renato Pariz [UNESP]Stella, Ariel EuridesRiccardi, Kátia [UNESP]Rigobelo, Everlon Cid [UNESP]Marin, José MoacirCarvalho, Marileda Bonafim [UNESP]Ávila, Fernando Antonio de [UNESP]2014-05-20T13:15:56Z2014-05-20T13:15:56Z2012-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article375-381application/pdfhttp://dx.doi.org/10.1590/S1517-83822012000100045Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 1, p. 375-381, 2012.1517-8382http://hdl.handle.net/11449/294210.1590/S1517-83822012000100045S1517-83822012000100045WOS:000303939800045S1517-83822012000100045.pdf301187271510908107466476017663900000-0002-9779-2213SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-06-07T13:02:37Zoai:repositorio.unesp.br:11449/2942Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:42:29.198198Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections |
title |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections |
spellingShingle |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections Maluta, Renato Pariz [UNESP] Biomarkers biotyping Eric-PCR UPEC |
title_short |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections |
title_full |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections |
title_fullStr |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections |
title_full_unstemmed |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections |
title_sort |
Phenotypical characterization and adhesin identification in Escherichia coli strains isolated from dogs with urinary tract infections |
author |
Maluta, Renato Pariz [UNESP] |
author_facet |
Maluta, Renato Pariz [UNESP] Stella, Ariel Eurides Riccardi, Kátia [UNESP] Rigobelo, Everlon Cid [UNESP] Marin, José Moacir Carvalho, Marileda Bonafim [UNESP] Ávila, Fernando Antonio de [UNESP] |
author_role |
author |
author2 |
Stella, Ariel Eurides Riccardi, Kátia [UNESP] Rigobelo, Everlon Cid [UNESP] Marin, José Moacir Carvalho, Marileda Bonafim [UNESP] Ávila, Fernando Antonio de [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Goiás (UFG) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Maluta, Renato Pariz [UNESP] Stella, Ariel Eurides Riccardi, Kátia [UNESP] Rigobelo, Everlon Cid [UNESP] Marin, José Moacir Carvalho, Marileda Bonafim [UNESP] Ávila, Fernando Antonio de [UNESP] |
dc.subject.por.fl_str_mv |
Biomarkers biotyping Eric-PCR UPEC |
topic |
Biomarkers biotyping Eric-PCR UPEC |
description |
Pathogenic strains of Escherichia coli are the most common bacteria associated with urinary tract infections in both humans and companion animals. Standard biochemical tests may be useful in demonstrating detailed phenotypical characteristics of these strains. Thirteen strains of E. coli isolated from dogs with UTIs were submitted to biochemical tests, serotyping for O and H antigens and antimicrobial resistance testing. Furthermore, the presence of papC, sfa, and afa genes was evaluated by PCR, and genetic relationships were established using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR). The antimicrobial that showed the highest resistance rate among the isolates was nalidixic acid (76.9%), followed by cephalotin (69.2%), sulfamethoxazole + trimethoprim (61.5%), tetracycline (61.5%), streptomycin (53.8%), ciprofloxacin (53.8%), ampicillin (46.2%), gentamicin (30.8%) and chloramphenicol (23.1%). No isolate was resistant either to meropenem or nitrofurantoin. Among the five clusters that were identified using ERIC-PCR, one cluster (A) had only one strain, which belonged to a serotype with zoonotic potential (O6:H31) and showed the genes papC+, sfa+, afa-. Strains with the genes papC-, sfa+, afa- were found in two other clusters (C and D), whereas all strains in clusters B and E possessed papC-, sfa-, afa- genes. Sucrose and raffinose phenotypic tests showed some ability in discriminating clusters A, B and C from clusters D and E. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-01 2014-05-20T13:15:56Z 2014-05-20T13:15:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822012000100045 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 1, p. 375-381, 2012. 1517-8382 http://hdl.handle.net/11449/2942 10.1590/S1517-83822012000100045 S1517-83822012000100045 WOS:000303939800045 S1517-83822012000100045.pdf 3011872715109081 0746647601766390 0000-0002-9779-2213 |
url |
http://dx.doi.org/10.1590/S1517-83822012000100045 http://hdl.handle.net/11449/2942 |
identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 1, p. 375-381, 2012. 1517-8382 10.1590/S1517-83822012000100045 S1517-83822012000100045 WOS:000303939800045 S1517-83822012000100045.pdf 3011872715109081 0746647601766390 0000-0002-9779-2213 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal of Microbiology 1.810 0,630 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
375-381 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129108525187072 |