New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ijppaw.2021.02.009 http://hdl.handle.net/11449/207431 |
Resumo: | The Amphibia are considered the most threatened vertebrate class globally, yet in Brazil they are also one of the more diverse and species rich groups. Although, in recent years there has been strong focus on amphibian related research, their parasites have not received the same attention. In Brazil, only a single species of Hepatozoon, namely H. leptodactyli (Lesage, 1908) Pessoa, 1970, has been described from anuran hosts. The present study aimed to describe three new species of Hepatozoon parasitising Leptodactylus labyrinthicus and Leptodactylus latrans from Mato Grosso State, Brazil. From 66 anurans screened for haemogregarines, four belonging to the Leptodactylidae were found positive for species of Hepatozoon. Based on the morphological analysis of peripheral blood gamonts and spleen and liver tissue meronts, three different morphotypes of Hepatozoon spp. were identified. Morphotype 1 (M1) and morphotype 2 (M2) in L. labyrinthicus and morphotype 3 (M3) in L. latrans. Molecular data based on partial 18S rDNA sequences revealed an interspecific divergence, between the species ranging from 0.43% to 1.16%. Phylogenetic analysis recovered isolates from the present study monophyletic with other isolates from Brazilian reptile and anuran hosts, sister to a clade comprising species isolated from African, North American and European reptile and anuran host species. Thus, using morphological and molecular analysis three new species infecting Brazilian Leptodactylidae anurans were identified and described. This study increases the knowledge of Brazilian anurans blood parasites and demonstrates the importance of using integrative approaches for diagnosis of hemoparasites. |
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New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans18S rDNAAnuranHaemogregarineHemoparasitePhylogenyThe Amphibia are considered the most threatened vertebrate class globally, yet in Brazil they are also one of the more diverse and species rich groups. Although, in recent years there has been strong focus on amphibian related research, their parasites have not received the same attention. In Brazil, only a single species of Hepatozoon, namely H. leptodactyli (Lesage, 1908) Pessoa, 1970, has been described from anuran hosts. The present study aimed to describe three new species of Hepatozoon parasitising Leptodactylus labyrinthicus and Leptodactylus latrans from Mato Grosso State, Brazil. From 66 anurans screened for haemogregarines, four belonging to the Leptodactylidae were found positive for species of Hepatozoon. Based on the morphological analysis of peripheral blood gamonts and spleen and liver tissue meronts, three different morphotypes of Hepatozoon spp. were identified. Morphotype 1 (M1) and morphotype 2 (M2) in L. labyrinthicus and morphotype 3 (M3) in L. latrans. Molecular data based on partial 18S rDNA sequences revealed an interspecific divergence, between the species ranging from 0.43% to 1.16%. Phylogenetic analysis recovered isolates from the present study monophyletic with other isolates from Brazilian reptile and anuran hosts, sister to a clade comprising species isolated from African, North American and European reptile and anuran host species. Thus, using morphological and molecular analysis three new species infecting Brazilian Leptodactylidae anurans were identified and described. This study increases the knowledge of Brazilian anurans blood parasites and demonstrates the importance of using integrative approaches for diagnosis of hemoparasites.National Research FoundationFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Setor de Parasitologia DBBVPZ Instituto de Biociências Universidade Estadual Paulista- UNESP, Distrito de Rubião JuniorUnit for Environmental Sciences and Management North-West University, Private Bag X6001Laboratório de Ensino e Pesquisa em Animais Silvestres Faculdade de Medicina Veterinária Universidade Federal de UberlândiaSetor de Parasitologia DBBVPZ Instituto de Biociências Universidade Estadual Paulista- UNESP, Distrito de Rubião JuniorNational Research Foundation: 129669FAPESP: 2016/50377–1FAPESP: 2018/00754–9FAPESP: 2018/09623–4CNPq: 309125/2017–0CNPq: 440496/2015–2Universidade Estadual Paulista (Unesp)North-West UniversityUniversidade Federal de Uberlândia (UFU)Úngari, Letícia Pereira [UNESP]Netherlands, Edward C.Quagliatto Santos, André LuizPaulino de Alcantara, Edna [UNESP]Emmerich, Enzo [UNESP]da Silva, Reinaldo José [UNESP]O'Dwyer, Lucia Helena [UNESP]2021-06-25T10:55:03Z2021-06-25T10:55:03Z2021-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article190-201http://dx.doi.org/10.1016/j.ijppaw.2021.02.009International Journal for Parasitology: Parasites and Wildlife, v. 14, p. 190-201.2213-2244http://hdl.handle.net/11449/20743110.1016/j.ijppaw.2021.02.0092-s2.0-85102379484Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal for Parasitology: Parasites and Wildlifeinfo:eu-repo/semantics/openAccess2021-10-23T17:16:33Zoai:repositorio.unesp.br:11449/207431Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T17:16:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans |
title |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans |
spellingShingle |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans Úngari, Letícia Pereira [UNESP] 18S rDNA Anuran Haemogregarine Hemoparasite Phylogeny |
title_short |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans |
title_full |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans |
title_fullStr |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans |
title_full_unstemmed |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans |
title_sort |
New insights on the diversity of Brazilian anuran blood parasites: With the description of three new species of Hepatozoon (Apicomplexa: Hepatozoidae) from Leptodactylidae anurans |
author |
Úngari, Letícia Pereira [UNESP] |
author_facet |
Úngari, Letícia Pereira [UNESP] Netherlands, Edward C. Quagliatto Santos, André Luiz Paulino de Alcantara, Edna [UNESP] Emmerich, Enzo [UNESP] da Silva, Reinaldo José [UNESP] O'Dwyer, Lucia Helena [UNESP] |
author_role |
author |
author2 |
Netherlands, Edward C. Quagliatto Santos, André Luiz Paulino de Alcantara, Edna [UNESP] Emmerich, Enzo [UNESP] da Silva, Reinaldo José [UNESP] O'Dwyer, Lucia Helena [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) North-West University Universidade Federal de Uberlândia (UFU) |
dc.contributor.author.fl_str_mv |
Úngari, Letícia Pereira [UNESP] Netherlands, Edward C. Quagliatto Santos, André Luiz Paulino de Alcantara, Edna [UNESP] Emmerich, Enzo [UNESP] da Silva, Reinaldo José [UNESP] O'Dwyer, Lucia Helena [UNESP] |
dc.subject.por.fl_str_mv |
18S rDNA Anuran Haemogregarine Hemoparasite Phylogeny |
topic |
18S rDNA Anuran Haemogregarine Hemoparasite Phylogeny |
description |
The Amphibia are considered the most threatened vertebrate class globally, yet in Brazil they are also one of the more diverse and species rich groups. Although, in recent years there has been strong focus on amphibian related research, their parasites have not received the same attention. In Brazil, only a single species of Hepatozoon, namely H. leptodactyli (Lesage, 1908) Pessoa, 1970, has been described from anuran hosts. The present study aimed to describe three new species of Hepatozoon parasitising Leptodactylus labyrinthicus and Leptodactylus latrans from Mato Grosso State, Brazil. From 66 anurans screened for haemogregarines, four belonging to the Leptodactylidae were found positive for species of Hepatozoon. Based on the morphological analysis of peripheral blood gamonts and spleen and liver tissue meronts, three different morphotypes of Hepatozoon spp. were identified. Morphotype 1 (M1) and morphotype 2 (M2) in L. labyrinthicus and morphotype 3 (M3) in L. latrans. Molecular data based on partial 18S rDNA sequences revealed an interspecific divergence, between the species ranging from 0.43% to 1.16%. Phylogenetic analysis recovered isolates from the present study monophyletic with other isolates from Brazilian reptile and anuran hosts, sister to a clade comprising species isolated from African, North American and European reptile and anuran host species. Thus, using morphological and molecular analysis three new species infecting Brazilian Leptodactylidae anurans were identified and described. This study increases the knowledge of Brazilian anurans blood parasites and demonstrates the importance of using integrative approaches for diagnosis of hemoparasites. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:55:03Z 2021-06-25T10:55:03Z 2021-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijppaw.2021.02.009 International Journal for Parasitology: Parasites and Wildlife, v. 14, p. 190-201. 2213-2244 http://hdl.handle.net/11449/207431 10.1016/j.ijppaw.2021.02.009 2-s2.0-85102379484 |
url |
http://dx.doi.org/10.1016/j.ijppaw.2021.02.009 http://hdl.handle.net/11449/207431 |
identifier_str_mv |
International Journal for Parasitology: Parasites and Wildlife, v. 14, p. 190-201. 2213-2244 10.1016/j.ijppaw.2021.02.009 2-s2.0-85102379484 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal for Parasitology: Parasites and Wildlife |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
190-201 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965728111067136 |