Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00198-017-3905-7 http://hdl.handle.net/11449/159482 |
Resumo: | The effects of jump training on bone structure before and after ovariectomy-induced osteopenia in rats were investigated. Jumping exercise induced favorable changes in bone mineral density, bone mechanical properties, and bone formation/resorption markers. This exercise is effective to prevent bone loss after ovariectomy even when osteopenia is already established. The present study investigated the effects of jump training on bone structure before and after ovariectomy-induced osteopenia in 80 10-week-old Wistar rats. Forty rats (prevention program) were randomly allocated to one of four equal groups (n = 10): sham-operated sedentary (SHAM-SEDp), ovariectomized (OVX) sedentary (OVX-SEDp), sham-operated exercised (SHAM-EXp), and OVX exercised (OVX-EXp). SHAM-EXp and OVX-EXp animals began training 3 days after surgery. Another 40 rats (treatment program) were randomly allocated into another four groups (n = 10): sham-operated sedentary (SHAM-SEDt), OVX sedentary (OVX-SEDt), sham-operated exercised (SHAM-EXt), and OVX exercised (OVX-EXt). SHAM-EXt and OVX-EXt animals began training 60 days after surgery. The rats in the exercised groups jumped 20 times/day, 5 days/week, to a height of 40 cm for 12 weeks. At the end of the experimental period, serum osteocalcin, follicle-stimulating hormone (FSH) dosage, dual X-ray absorptiometry (DXA), histomorphometry, and biomechanical tests were analyzed. The OVX groups showed higher values of FSH and body weight (p < 0.05). DXA showed that jump training significantly increased bone mineral density of the femur and fifth lumbar vertebra (p < 0.05). The stiffness of the left femur and fifth lumbar vertebra in the exercised groups was greater than that of the sedentary groups (p < 0.05). Ovariectomy induced significant difference in bone volume (BV/TV, percent), trabecular separation (Tb.Sp, micrometer), and trabecular number (Tb.N, per millimeter) (p < 0.05) compared to sham operation. Jump training in the OVX group induced significant differences in BV/TV, Tb.Sp, and Tb.N and decreased osteoblast number per bone perimeter (p < 0.05) compared with OVX nontraining, in the prevention groups. Osteocalcin dosage showed higher values in the exercised groups (p < 0.05). Jumping exercise induced favorable changes in bone mineral density, bone mechanical properties, and bone formation/resorption markers. Jump training is effective to prevent bone loss after ovariectomy even when osteopenia is already established. |
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Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopeniaExercise therapyOsteopeniaOvariectomyRehabilitation medicineThe effects of jump training on bone structure before and after ovariectomy-induced osteopenia in rats were investigated. Jumping exercise induced favorable changes in bone mineral density, bone mechanical properties, and bone formation/resorption markers. This exercise is effective to prevent bone loss after ovariectomy even when osteopenia is already established. The present study investigated the effects of jump training on bone structure before and after ovariectomy-induced osteopenia in 80 10-week-old Wistar rats. Forty rats (prevention program) were randomly allocated to one of four equal groups (n = 10): sham-operated sedentary (SHAM-SEDp), ovariectomized (OVX) sedentary (OVX-SEDp), sham-operated exercised (SHAM-EXp), and OVX exercised (OVX-EXp). SHAM-EXp and OVX-EXp animals began training 3 days after surgery. Another 40 rats (treatment program) were randomly allocated into another four groups (n = 10): sham-operated sedentary (SHAM-SEDt), OVX sedentary (OVX-SEDt), sham-operated exercised (SHAM-EXt), and OVX exercised (OVX-EXt). SHAM-EXt and OVX-EXt animals began training 60 days after surgery. The rats in the exercised groups jumped 20 times/day, 5 days/week, to a height of 40 cm for 12 weeks. At the end of the experimental period, serum osteocalcin, follicle-stimulating hormone (FSH) dosage, dual X-ray absorptiometry (DXA), histomorphometry, and biomechanical tests were analyzed. The OVX groups showed higher values of FSH and body weight (p < 0.05). DXA showed that jump training significantly increased bone mineral density of the femur and fifth lumbar vertebra (p < 0.05). The stiffness of the left femur and fifth lumbar vertebra in the exercised groups was greater than that of the sedentary groups (p < 0.05). Ovariectomy induced significant difference in bone volume (BV/TV, percent), trabecular separation (Tb.Sp, micrometer), and trabecular number (Tb.N, per millimeter) (p < 0.05) compared to sham operation. Jump training in the OVX group induced significant differences in BV/TV, Tb.Sp, and Tb.N and decreased osteoblast number per bone perimeter (p < 0.05) compared with OVX nontraining, in the prevention groups. Osteocalcin dosage showed higher values in the exercised groups (p < 0.05). Jumping exercise induced favorable changes in bone mineral density, bone mechanical properties, and bone formation/resorption markers. Jump training is effective to prevent bone loss after ovariectomy even when osteopenia is already established.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Santa Catarina State Univ, Dept Physiotherapy, Florianopolis, SC, BrazilUniv Sao Paulo, Sch Med Ribeirao Preto, Dept Biomech Med & Rehabil Locomotor Apparatus, 3900 Bandeirantes Ave, Sao Paulo, BrazilSao Paulo State Univ, Sch Vet Med, Dept Anim Support Prod & Hlth, Sao Paulo, BrazilUniv Sao Paulo, Sch Med Ribeirao Preto, Dept Internal Med, Sao Paulo, BrazilUniv Salerno, Fac Med & Surg, Dept Musculoskeletal Disorders, Salerno, ItalyMile End Hosp, Barts & London Sch Med & Dent, Ctr Sports & Exercise Med, 275 Bancroft Rd, London E1 4DG, EnglandSao Paulo State Univ, Sch Vet Med, Dept Anim Support Prod & Hlth, Sao Paulo, BrazilFAPESP: 2008/10785-7SpringerSanta Catarina State UnivUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Univ SalernoMile End HospOkubo, R.Sanada, L. S.Castania, V. A.Louzada, M. J. Q. [UNESP]Paula, F. J. A. deMaffulli, N.Shimano, A. C.2018-11-26T15:43:58Z2018-11-26T15:43:58Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1461-1471application/pdfhttp://dx.doi.org/10.1007/s00198-017-3905-7Osteoporosis International. London: Springer London Ltd, v. 28, n. 4, p. 1461-1471, 2017.0937-941Xhttp://hdl.handle.net/11449/15948210.1007/s00198-017-3905-7WOS:000398518800031WOS000398518800031.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOsteoporosis Internationalinfo:eu-repo/semantics/openAccess2023-10-20T06:10:09Zoai:repositorio.unesp.br:11449/159482Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-20T06:10:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia |
title |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia |
spellingShingle |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia Okubo, R. Exercise therapy Osteopenia Ovariectomy Rehabilitation medicine |
title_short |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia |
title_full |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia |
title_fullStr |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia |
title_full_unstemmed |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia |
title_sort |
Jumping exercise preserves bone mineral density and mechanical properties in osteopenic ovariectomized rats even following established osteopenia |
author |
Okubo, R. |
author_facet |
Okubo, R. Sanada, L. S. Castania, V. A. Louzada, M. J. Q. [UNESP] Paula, F. J. A. de Maffulli, N. Shimano, A. C. |
author_role |
author |
author2 |
Sanada, L. S. Castania, V. A. Louzada, M. J. Q. [UNESP] Paula, F. J. A. de Maffulli, N. Shimano, A. C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Santa Catarina State Univ Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) Univ Salerno Mile End Hosp |
dc.contributor.author.fl_str_mv |
Okubo, R. Sanada, L. S. Castania, V. A. Louzada, M. J. Q. [UNESP] Paula, F. J. A. de Maffulli, N. Shimano, A. C. |
dc.subject.por.fl_str_mv |
Exercise therapy Osteopenia Ovariectomy Rehabilitation medicine |
topic |
Exercise therapy Osteopenia Ovariectomy Rehabilitation medicine |
description |
The effects of jump training on bone structure before and after ovariectomy-induced osteopenia in rats were investigated. Jumping exercise induced favorable changes in bone mineral density, bone mechanical properties, and bone formation/resorption markers. This exercise is effective to prevent bone loss after ovariectomy even when osteopenia is already established. The present study investigated the effects of jump training on bone structure before and after ovariectomy-induced osteopenia in 80 10-week-old Wistar rats. Forty rats (prevention program) were randomly allocated to one of four equal groups (n = 10): sham-operated sedentary (SHAM-SEDp), ovariectomized (OVX) sedentary (OVX-SEDp), sham-operated exercised (SHAM-EXp), and OVX exercised (OVX-EXp). SHAM-EXp and OVX-EXp animals began training 3 days after surgery. Another 40 rats (treatment program) were randomly allocated into another four groups (n = 10): sham-operated sedentary (SHAM-SEDt), OVX sedentary (OVX-SEDt), sham-operated exercised (SHAM-EXt), and OVX exercised (OVX-EXt). SHAM-EXt and OVX-EXt animals began training 60 days after surgery. The rats in the exercised groups jumped 20 times/day, 5 days/week, to a height of 40 cm for 12 weeks. At the end of the experimental period, serum osteocalcin, follicle-stimulating hormone (FSH) dosage, dual X-ray absorptiometry (DXA), histomorphometry, and biomechanical tests were analyzed. The OVX groups showed higher values of FSH and body weight (p < 0.05). DXA showed that jump training significantly increased bone mineral density of the femur and fifth lumbar vertebra (p < 0.05). The stiffness of the left femur and fifth lumbar vertebra in the exercised groups was greater than that of the sedentary groups (p < 0.05). Ovariectomy induced significant difference in bone volume (BV/TV, percent), trabecular separation (Tb.Sp, micrometer), and trabecular number (Tb.N, per millimeter) (p < 0.05) compared to sham operation. Jump training in the OVX group induced significant differences in BV/TV, Tb.Sp, and Tb.N and decreased osteoblast number per bone perimeter (p < 0.05) compared with OVX nontraining, in the prevention groups. Osteocalcin dosage showed higher values in the exercised groups (p < 0.05). Jumping exercise induced favorable changes in bone mineral density, bone mechanical properties, and bone formation/resorption markers. Jump training is effective to prevent bone loss after ovariectomy even when osteopenia is already established. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-11-26T15:43:58Z 2018-11-26T15:43:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00198-017-3905-7 Osteoporosis International. London: Springer London Ltd, v. 28, n. 4, p. 1461-1471, 2017. 0937-941X http://hdl.handle.net/11449/159482 10.1007/s00198-017-3905-7 WOS:000398518800031 WOS000398518800031.pdf |
url |
http://dx.doi.org/10.1007/s00198-017-3905-7 http://hdl.handle.net/11449/159482 |
identifier_str_mv |
Osteoporosis International. London: Springer London Ltd, v. 28, n. 4, p. 1461-1471, 2017. 0937-941X 10.1007/s00198-017-3905-7 WOS:000398518800031 WOS000398518800031.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Osteoporosis International |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1461-1471 application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789458114805760 |