Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992007000300003 http://hdl.handle.net/11449/69931 |
Resumo: | Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural venom; and GIIV, inoculated with 0.5mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase (CK), aspartate amino-transferase (AST) and alanine amino-transferase (ALT); clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom. |
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Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venomBothrops jararacaCobaltGoatsRadiationAnimaliaBothropsCapra hircusSerpentesVenoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural venom; and GIIV, inoculated with 0.5mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase (CK), aspartate amino-transferase (AST) and alanine amino-transferase (ALT); clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom.Veterinary Hospital Uberaba School of Veterinary Medicine UNIUBE, Uberaba, Minas Gerais StateVeterinary Clinics School of Veterinary Medicine and Animal Husbandry UNESP, Botucatu, São Paulo StateCenter for the Study of Venoms and Venomous Animals CEVAP UNESP, São Paulo State, Rua Bahia, 870, 38050-130, Uberaba, Minas GeraisVeterinary Clinics School of Veterinary Medicine and Animal Husbandry UNESP, Botucatu, São Paulo StateCenter for the Study of Venoms and Venomous Animals CEVAP UNESP, São Paulo StateUNIUBEUniversidade Estadual Paulista (Unesp)Lucas de Oliveira, Pedro CarlosSakate, M. [UNESP]Madruga, R. A.Barbosa, N. P U2014-05-27T11:22:37Z2014-05-27T11:22:37Z2007-10-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article576-597application/pdfhttp://dx.doi.org/10.1590/S1678-91992007000300003Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 13, n. 3, p. 576-597, 2007.1678-9199http://hdl.handle.net/11449/6993110.1590/S1678-91992007000300003S1678-91992007000300003WOS:0002496942000032-s2.0-348488942592-s2.0-34848894259.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-12-24T06:15:38Zoai:repositorio.unesp.br:11449/69931Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-24T06:15:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
title |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
spellingShingle |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom Lucas de Oliveira, Pedro Carlos Bothrops jararaca Cobalt Goats Radiation Animalia Bothrops Capra hircus Serpentes |
title_short |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
title_full |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
title_fullStr |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
title_full_unstemmed |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
title_sort |
Biochemical and hematological study of goats envenomed with natural and 60Co-irradiated bothropic venom |
author |
Lucas de Oliveira, Pedro Carlos |
author_facet |
Lucas de Oliveira, Pedro Carlos Sakate, M. [UNESP] Madruga, R. A. Barbosa, N. P U |
author_role |
author |
author2 |
Sakate, M. [UNESP] Madruga, R. A. Barbosa, N. P U |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
UNIUBE Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lucas de Oliveira, Pedro Carlos Sakate, M. [UNESP] Madruga, R. A. Barbosa, N. P U |
dc.subject.por.fl_str_mv |
Bothrops jararaca Cobalt Goats Radiation Animalia Bothrops Capra hircus Serpentes |
topic |
Bothrops jararaca Cobalt Goats Radiation Animalia Bothrops Capra hircus Serpentes |
description |
Venoms from snakes of the Bothrops genus are proteolytic, coagulant, hemorrhagic and nephrotoxic, causing edema, necrosis, hemorrhage and intense pain at the bite site, besides systemic alterations. Many adjuvants have been added to the venom used in the sensitization of antiserum-producer animals to increase antigenic induction and reduce the envenomation pathological effects. Gamma radiation from 60Co has been used as an attenuating agent of the venoms toxic properties. The main objective was to study, comparatively, clinical and laboratory aspects of goats inoculated with bothropic (Bothrops jararaca) venom, natural and irradiated from a 60Co source. Twelve goats were divided into two groups of six animals: GINV, inoculated with 0.5mg/kg of natural venom; and GIIV, inoculated with 0.5mg/kg of irradiated venom. Blood samples were collected immediately before and one, two, seven, and thirty days after venom injection. Local lesions were daily evaluated. The following exams were carried out: blood tests; biochemical tests of urea, creatinine, creatine kinase (CK), aspartate amino-transferase (AST) and alanine amino-transferase (ALT); clotting time; platelets count; and total serum immunoglobulin measurement. In the conditions of the present experiment, irradiated venom was less aggressive and more immunogenic than natural venom. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-08 2014-05-27T11:22:37Z 2014-05-27T11:22:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992007000300003 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 13, n. 3, p. 576-597, 2007. 1678-9199 http://hdl.handle.net/11449/69931 10.1590/S1678-91992007000300003 S1678-91992007000300003 WOS:000249694200003 2-s2.0-34848894259 2-s2.0-34848894259.pdf |
url |
http://dx.doi.org/10.1590/S1678-91992007000300003 http://hdl.handle.net/11449/69931 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 13, n. 3, p. 576-597, 2007. 1678-9199 10.1590/S1678-91992007000300003 S1678-91992007000300003 WOS:000249694200003 2-s2.0-34848894259 2-s2.0-34848894259.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
576-597 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965390001930240 |