Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.rvsc.2020.11.021 http://hdl.handle.net/11449/206953 |
Resumo: | Visceral Leishmaniasis (VL) is a neglected tropical disease, caused by L. infantum in the New World, where dogs are the main reservoir. These parasites can regulate host immune response through miRNA differential expression in the early stages of infection; however such early response has not yet been investigated in the canine model. PBMC from healthy dogs were exposed to L. infantum in vitro and microarray analysis showed an upregulation of miR-206, miR-302d, miR-433, miR-214, miR-493, miR-514, miR-1835, miR-210, miR-539, miR-432, miR-188, miR-345 and downregulation of miR-489 and miR-503 in comparison to non-exposed control cells, at 24 h post-exposure. In silico target prediction showed that the upregulated miRNAs target 1541 genes, which can modulate important pathways involved in the early immune responses, like the “MAPK signaling pathway”, one of the most relevant pathways to Leishmania survival inside host cells. These findings shed light on parasite modulation of host immunity following Leishmania infection, which in turn can be explored for drug development. |
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Repositório Institucional da UNESP |
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Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitroDogLeishmaniaMAPKmiRNAVisceral Leishmaniasis (VL) is a neglected tropical disease, caused by L. infantum in the New World, where dogs are the main reservoir. These parasites can regulate host immune response through miRNA differential expression in the early stages of infection; however such early response has not yet been investigated in the canine model. PBMC from healthy dogs were exposed to L. infantum in vitro and microarray analysis showed an upregulation of miR-206, miR-302d, miR-433, miR-214, miR-493, miR-514, miR-1835, miR-210, miR-539, miR-432, miR-188, miR-345 and downregulation of miR-489 and miR-503 in comparison to non-exposed control cells, at 24 h post-exposure. In silico target prediction showed that the upregulated miRNAs target 1541 genes, which can modulate important pathways involved in the early immune responses, like the “MAPK signaling pathway”, one of the most relevant pathways to Leishmania survival inside host cells. These findings shed light on parasite modulation of host immunity following Leishmania infection, which in turn can be explored for drug development.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine AraçatubaDepartment of Support Production and Animal Health São Paulo State University (UNESP) School of Veterinary Medicine AraçatubaDepartment of Surgery and Animal Reproduction São Paulo State University (UNESP) School of Veterinary Medicine AraçatubaDepartment of Support Production and Animal Health São Paulo State University (UNESP) School of Veterinary Medicine AraçatubaFAPESP: 2015/16101-6FAPESP: 2015/16972-7Universidade Estadual Paulista (Unesp)Soares, Matheus Fujimura [UNESP]Melo, Larissa Martins [UNESP]Bragato, Jaqueline Poleto [UNESP]Furlan, Amanda de Oliveira [UNESP]Scaramele, Natália Francisco [UNESP]Lopes, Flávia Lombardi [UNESP]Lima, Valéria Marçal Felix de [UNESP]2021-06-25T10:46:36Z2021-06-25T10:46:36Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article58-63http://dx.doi.org/10.1016/j.rvsc.2020.11.021Research in Veterinary Science, v. 134, p. 58-63.1532-26610034-5288http://hdl.handle.net/11449/20695310.1016/j.rvsc.2020.11.0212-s2.0-85097456540Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengResearch in Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-23T15:48:30Zoai:repositorio.unesp.br:11449/206953Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T15:48:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro |
title |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro |
spellingShingle |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro Soares, Matheus Fujimura [UNESP] Dog Leishmania MAPK miRNA |
title_short |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro |
title_full |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro |
title_fullStr |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro |
title_full_unstemmed |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro |
title_sort |
Differential expression of miRNAs in canine peripheral blood mononuclear cells (PBMC) exposed to Leishmania infantum in vitro |
author |
Soares, Matheus Fujimura [UNESP] |
author_facet |
Soares, Matheus Fujimura [UNESP] Melo, Larissa Martins [UNESP] Bragato, Jaqueline Poleto [UNESP] Furlan, Amanda de Oliveira [UNESP] Scaramele, Natália Francisco [UNESP] Lopes, Flávia Lombardi [UNESP] Lima, Valéria Marçal Felix de [UNESP] |
author_role |
author |
author2 |
Melo, Larissa Martins [UNESP] Bragato, Jaqueline Poleto [UNESP] Furlan, Amanda de Oliveira [UNESP] Scaramele, Natália Francisco [UNESP] Lopes, Flávia Lombardi [UNESP] Lima, Valéria Marçal Felix de [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Soares, Matheus Fujimura [UNESP] Melo, Larissa Martins [UNESP] Bragato, Jaqueline Poleto [UNESP] Furlan, Amanda de Oliveira [UNESP] Scaramele, Natália Francisco [UNESP] Lopes, Flávia Lombardi [UNESP] Lima, Valéria Marçal Felix de [UNESP] |
dc.subject.por.fl_str_mv |
Dog Leishmania MAPK miRNA |
topic |
Dog Leishmania MAPK miRNA |
description |
Visceral Leishmaniasis (VL) is a neglected tropical disease, caused by L. infantum in the New World, where dogs are the main reservoir. These parasites can regulate host immune response through miRNA differential expression in the early stages of infection; however such early response has not yet been investigated in the canine model. PBMC from healthy dogs were exposed to L. infantum in vitro and microarray analysis showed an upregulation of miR-206, miR-302d, miR-433, miR-214, miR-493, miR-514, miR-1835, miR-210, miR-539, miR-432, miR-188, miR-345 and downregulation of miR-489 and miR-503 in comparison to non-exposed control cells, at 24 h post-exposure. In silico target prediction showed that the upregulated miRNAs target 1541 genes, which can modulate important pathways involved in the early immune responses, like the “MAPK signaling pathway”, one of the most relevant pathways to Leishmania survival inside host cells. These findings shed light on parasite modulation of host immunity following Leishmania infection, which in turn can be explored for drug development. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:46:36Z 2021-06-25T10:46:36Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.rvsc.2020.11.021 Research in Veterinary Science, v. 134, p. 58-63. 1532-2661 0034-5288 http://hdl.handle.net/11449/206953 10.1016/j.rvsc.2020.11.021 2-s2.0-85097456540 |
url |
http://dx.doi.org/10.1016/j.rvsc.2020.11.021 http://hdl.handle.net/11449/206953 |
identifier_str_mv |
Research in Veterinary Science, v. 134, p. 58-63. 1532-2661 0034-5288 10.1016/j.rvsc.2020.11.021 2-s2.0-85097456540 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Research in Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
58-63 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965046428663808 |