Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.2341/19-013-L http://hdl.handle.net/11449/206822 |
Resumo: | Objective: The aim of this study was to evaluate the influence of the presence of pigments in tooth structures on the trans-enamel and trans-dentin diffusion of hydrogen peroxide (H2O2) and its cytotoxicity after carrying out an in-office bleaching therapy. Methods and Materials: A bleaching gel with 35% H2O2 was applied for 45 minutes (three times for 15 minutes) on enamel and dentin discs (n=6), either previously submitted to the intrinsic pigmentation protocol with a concentrated solution of black tea, or not, defining the following groups: G1, unbleached untreated discs (control 1); G2, unbleached pigmented discs (control 2); G3, bleached untreated discs; G4, bleached pigmented discs. The discs were adapted to artificial pulp chambers, which were placed in wells of 24-well plates containing 1 mL culture medium (Dulbecco’s modified Eagle’s medium [DMEM]). After applying the bleaching gel on enamel, the extracts (DMEM + components of bleaching gel that diffused through the discs) were collected and then applied on the cultured MDPC-23 odontoblast-like cells. Cell viability (methyl tetrazolium assay and Live & Dead, Calcein AM, and ethidium homodimer-1 [EthD-1] probes), the amount of H2O2 that diffused through enamel and dentin (leuco-crystal violet product), and the H2O2-mediated oxidative cell stress (SOx) and components of degradation were assessed (analysis of variance/Tukey; a=0.05). Results: There was no significant difference between the groups G1 and G2 for all the parameters tested (p.0.05). Reduction in the trans-enamel and trans-dentin diffusion of H2O2 occurred for G4 in comparison with G3. Significantly lower cell viability associated with greater oxidative stress was observed for G3 (p,0.05). Therefore, in-office tooth bleaching therapy performed in pigmented samples caused lower cytotoxic effects compared with untreated samples submitted to the same esthetic procedure (p,0.05). Conclusion: According to the methodology used in this investigation, the authors concluded that the presence of pigments in hard tooth structures decreases the trans-enamel and trans-dentin diffusion of H2O2 and the toxicity to pulp cells of an in-office bleaching gel with 35% H2O2. |
id |
UNSP_d32750424dbf8fa2dead64e046119892 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/206822 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleachingObjective: The aim of this study was to evaluate the influence of the presence of pigments in tooth structures on the trans-enamel and trans-dentin diffusion of hydrogen peroxide (H2O2) and its cytotoxicity after carrying out an in-office bleaching therapy. Methods and Materials: A bleaching gel with 35% H2O2 was applied for 45 minutes (three times for 15 minutes) on enamel and dentin discs (n=6), either previously submitted to the intrinsic pigmentation protocol with a concentrated solution of black tea, or not, defining the following groups: G1, unbleached untreated discs (control 1); G2, unbleached pigmented discs (control 2); G3, bleached untreated discs; G4, bleached pigmented discs. The discs were adapted to artificial pulp chambers, which were placed in wells of 24-well plates containing 1 mL culture medium (Dulbecco’s modified Eagle’s medium [DMEM]). After applying the bleaching gel on enamel, the extracts (DMEM + components of bleaching gel that diffused through the discs) were collected and then applied on the cultured MDPC-23 odontoblast-like cells. Cell viability (methyl tetrazolium assay and Live & Dead, Calcein AM, and ethidium homodimer-1 [EthD-1] probes), the amount of H2O2 that diffused through enamel and dentin (leuco-crystal violet product), and the H2O2-mediated oxidative cell stress (SOx) and components of degradation were assessed (analysis of variance/Tukey; a=0.05). Results: There was no significant difference between the groups G1 and G2 for all the parameters tested (p.0.05). Reduction in the trans-enamel and trans-dentin diffusion of H2O2 occurred for G4 in comparison with G3. Significantly lower cell viability associated with greater oxidative stress was observed for G3 (p,0.05). Therefore, in-office tooth bleaching therapy performed in pigmented samples caused lower cytotoxic effects compared with untreated samples submitted to the same esthetic procedure (p,0.05). Conclusion: According to the methodology used in this investigation, the authors concluded that the presence of pigments in hard tooth structures decreases the trans-enamel and trans-dentin diffusion of H2O2 and the toxicity to pulp cells of an in-office bleaching gel with 35% H2O2.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Dental Materials and Prosthodontics Araraquara School of Dentistry University Estadual Paulista–UNESPDepartment of Operative Dentistry Endodontics and Dental Materials Bauru School of Dentistry University de São Paulo–USPDepartment of Restorative Dentistry Araçatuba School of Dentistry University Estadual Paulista–UNESPDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista–UNESPDepartment of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista–UNESPDepartment of Dental Materials and Prosthodontics Araraquara School of Dentistry University Estadual Paulista–UNESPDepartment of Restorative Dentistry Araçatuba School of Dentistry University Estadual Paulista–UNESPDepartment of Orthodontics and Pediatric Dentistry Araraquara School of Dentistry University Estadual Paulista–UNESPDepartment of Physiology and Pathology Araraquara School of Dentistry University Estadual Paulista–UNESPFAPESP: 2015/21770-4FAPESP: 2016/10928-9CNPq: 303599/2014-6CNPq: 443153/2014-0Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)de Oliveira Duque, C. C. [UNESP]Soares, D. G.Briso, A. L.F. [UNESP]Ortecho-Zuta, U. [UNESP]de Oliveira Ribeiro, R. A. [UNESP]Hebling, J. [UNESP]de Souza Costa, C. A. [UNESP]2021-06-25T10:44:24Z2021-06-25T10:44:24Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article632-642http://dx.doi.org/10.2341/19-013-LOperative Dentistry, v. 45, n. 6, p. 632-642, 2020.1559-28630361-7734http://hdl.handle.net/11449/20682210.2341/19-013-L2-s2.0-85095991177Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOperative Dentistryinfo:eu-repo/semantics/openAccess2021-10-23T10:18:20Zoai:repositorio.unesp.br:11449/206822Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:18:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching |
title |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching |
spellingShingle |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching de Oliveira Duque, C. C. [UNESP] |
title_short |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching |
title_full |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching |
title_fullStr |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching |
title_full_unstemmed |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching |
title_sort |
Influence of tooth pigmentation on H2O2 diffusion and its cytotoxicity after in-office tooth bleaching |
author |
de Oliveira Duque, C. C. [UNESP] |
author_facet |
de Oliveira Duque, C. C. [UNESP] Soares, D. G. Briso, A. L.F. [UNESP] Ortecho-Zuta, U. [UNESP] de Oliveira Ribeiro, R. A. [UNESP] Hebling, J. [UNESP] de Souza Costa, C. A. [UNESP] |
author_role |
author |
author2 |
Soares, D. G. Briso, A. L.F. [UNESP] Ortecho-Zuta, U. [UNESP] de Oliveira Ribeiro, R. A. [UNESP] Hebling, J. [UNESP] de Souza Costa, C. A. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
de Oliveira Duque, C. C. [UNESP] Soares, D. G. Briso, A. L.F. [UNESP] Ortecho-Zuta, U. [UNESP] de Oliveira Ribeiro, R. A. [UNESP] Hebling, J. [UNESP] de Souza Costa, C. A. [UNESP] |
description |
Objective: The aim of this study was to evaluate the influence of the presence of pigments in tooth structures on the trans-enamel and trans-dentin diffusion of hydrogen peroxide (H2O2) and its cytotoxicity after carrying out an in-office bleaching therapy. Methods and Materials: A bleaching gel with 35% H2O2 was applied for 45 minutes (three times for 15 minutes) on enamel and dentin discs (n=6), either previously submitted to the intrinsic pigmentation protocol with a concentrated solution of black tea, or not, defining the following groups: G1, unbleached untreated discs (control 1); G2, unbleached pigmented discs (control 2); G3, bleached untreated discs; G4, bleached pigmented discs. The discs were adapted to artificial pulp chambers, which were placed in wells of 24-well plates containing 1 mL culture medium (Dulbecco’s modified Eagle’s medium [DMEM]). After applying the bleaching gel on enamel, the extracts (DMEM + components of bleaching gel that diffused through the discs) were collected and then applied on the cultured MDPC-23 odontoblast-like cells. Cell viability (methyl tetrazolium assay and Live & Dead, Calcein AM, and ethidium homodimer-1 [EthD-1] probes), the amount of H2O2 that diffused through enamel and dentin (leuco-crystal violet product), and the H2O2-mediated oxidative cell stress (SOx) and components of degradation were assessed (analysis of variance/Tukey; a=0.05). Results: There was no significant difference between the groups G1 and G2 for all the parameters tested (p.0.05). Reduction in the trans-enamel and trans-dentin diffusion of H2O2 occurred for G4 in comparison with G3. Significantly lower cell viability associated with greater oxidative stress was observed for G3 (p,0.05). Therefore, in-office tooth bleaching therapy performed in pigmented samples caused lower cytotoxic effects compared with untreated samples submitted to the same esthetic procedure (p,0.05). Conclusion: According to the methodology used in this investigation, the authors concluded that the presence of pigments in hard tooth structures decreases the trans-enamel and trans-dentin diffusion of H2O2 and the toxicity to pulp cells of an in-office bleaching gel with 35% H2O2. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-01 2021-06-25T10:44:24Z 2021-06-25T10:44:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.2341/19-013-L Operative Dentistry, v. 45, n. 6, p. 632-642, 2020. 1559-2863 0361-7734 http://hdl.handle.net/11449/206822 10.2341/19-013-L 2-s2.0-85095991177 |
url |
http://dx.doi.org/10.2341/19-013-L http://hdl.handle.net/11449/206822 |
identifier_str_mv |
Operative Dentistry, v. 45, n. 6, p. 632-642, 2020. 1559-2863 0361-7734 10.2341/19-013-L 2-s2.0-85095991177 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Operative Dentistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
632-642 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789469395386368 |