Influence of forskolin supplementation on embryos produced in vitro
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2019.01.004 http://hdl.handle.net/11449/190028 |
Resumo: | The objective of this study was to evaluate the effects of forskolin supplementation of the culture medium on the development, lipid content, and transcript levels genes of in vitro-produced bovine embryos. Ovaries (n = 420) from Nelore cows were obtained from the slaughterhouse. On day 5 (D5) of in vitro culture, the embryos in the experimental group were treated with 10 µM of forskolin, and the control group embryos were not treated. The evaluation was performed based on blastocyst and hatching rates on D7 and D10. For the evaluation of lipid deposition, blastocysts (D7; n = 10/group) were stained with the Sudan black B. Additionally, 47 genes were analyzed with RTq-PCR. The embryo lipid content (gray intensity) was similar (P > 0.05) between the forskolin (772 ± 4.8) and control (883 ± 5.2) groups. The mRNA transcript levels of GPX1, HAND1, POU5F1 and HP1 were increased in the control group (P = 0.06). The mRNA transcript level of SREBF1 was equal (P = 0.06) in both treatments, and those of AKR1, IFNT2 (P = 0.06) and SCD (P = 0.08) were increased in forskolin-derived embryos when compared with control embryos. There was no difference (P > 0.05) in the blastocyst production rate between the forskolin (37.9%) and control (39.5%) groups. Interestingly, the blastocyst hatching rate at D10 was higher (P = 0.09) in the forskolin group (62.9%) than in the control group (48.4%). |
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Repositório Institucional da UNESP |
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spelling |
Influence of forskolin supplementation on embryos produced in vitroBovineForskolinGene expressionIVEP, Lipid contentThe objective of this study was to evaluate the effects of forskolin supplementation of the culture medium on the development, lipid content, and transcript levels genes of in vitro-produced bovine embryos. Ovaries (n = 420) from Nelore cows were obtained from the slaughterhouse. On day 5 (D5) of in vitro culture, the embryos in the experimental group were treated with 10 µM of forskolin, and the control group embryos were not treated. The evaluation was performed based on blastocyst and hatching rates on D7 and D10. For the evaluation of lipid deposition, blastocysts (D7; n = 10/group) were stained with the Sudan black B. Additionally, 47 genes were analyzed with RTq-PCR. The embryo lipid content (gray intensity) was similar (P > 0.05) between the forskolin (772 ± 4.8) and control (883 ± 5.2) groups. The mRNA transcript levels of GPX1, HAND1, POU5F1 and HP1 were increased in the control group (P = 0.06). The mRNA transcript level of SREBF1 was equal (P = 0.06) in both treatments, and those of AKR1, IFNT2 (P = 0.06) and SCD (P = 0.08) were increased in forskolin-derived embryos when compared with control embryos. There was no difference (P > 0.05) in the blastocyst production rate between the forskolin (37.9%) and control (39.5%) groups. Interestingly, the blastocyst hatching rate at D10 was higher (P = 0.09) in the forskolin group (62.9%) than in the control group (48.4%).University of Londrina (UEL) Laboratory of Animal ReproductionSão Paulo State University (UNESP) Institute of Biosciences Department of PharmacologySão Paulo State University (UNESP) School of Sciences Humanities and Languages Department of Biological ScienceSão Paulo State University (UNESP) Institute of Biosciences Department of PharmacologySão Paulo State University (UNESP) School of Sciences Humanities and Languages Department of Biological ScienceLaboratory of Animal ReproductionUniversidade Estadual Paulista (Unesp)Costa, Camila BortolieroLunardelli, Paula AlvaresBizarro-Silva, CamilaFontes, Patricia Kubo [UNESP]Nogueira, Marcelo Fábio Gouveia [UNESP]Seneda, Marcelo Marcondes2019-10-06T16:59:55Z2019-10-06T16:59:55Z2019-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15-18http://dx.doi.org/10.1016/j.livsci.2019.01.004Livestock Science, v. 221, p. 15-18.1871-1413http://hdl.handle.net/11449/19002810.1016/j.livsci.2019.01.0042-s2.0-850597845923734933152414412Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Scienceinfo:eu-repo/semantics/openAccess2021-10-23T16:36:51Zoai:repositorio.unesp.br:11449/190028Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T16:36:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of forskolin supplementation on embryos produced in vitro |
title |
Influence of forskolin supplementation on embryos produced in vitro |
spellingShingle |
Influence of forskolin supplementation on embryos produced in vitro Costa, Camila Bortoliero Bovine Forskolin Gene expression IVEP, Lipid content |
title_short |
Influence of forskolin supplementation on embryos produced in vitro |
title_full |
Influence of forskolin supplementation on embryos produced in vitro |
title_fullStr |
Influence of forskolin supplementation on embryos produced in vitro |
title_full_unstemmed |
Influence of forskolin supplementation on embryos produced in vitro |
title_sort |
Influence of forskolin supplementation on embryos produced in vitro |
author |
Costa, Camila Bortoliero |
author_facet |
Costa, Camila Bortoliero Lunardelli, Paula Alvares Bizarro-Silva, Camila Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Seneda, Marcelo Marcondes |
author_role |
author |
author2 |
Lunardelli, Paula Alvares Bizarro-Silva, Camila Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Seneda, Marcelo Marcondes |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Laboratory of Animal Reproduction Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Costa, Camila Bortoliero Lunardelli, Paula Alvares Bizarro-Silva, Camila Fontes, Patricia Kubo [UNESP] Nogueira, Marcelo Fábio Gouveia [UNESP] Seneda, Marcelo Marcondes |
dc.subject.por.fl_str_mv |
Bovine Forskolin Gene expression IVEP, Lipid content |
topic |
Bovine Forskolin Gene expression IVEP, Lipid content |
description |
The objective of this study was to evaluate the effects of forskolin supplementation of the culture medium on the development, lipid content, and transcript levels genes of in vitro-produced bovine embryos. Ovaries (n = 420) from Nelore cows were obtained from the slaughterhouse. On day 5 (D5) of in vitro culture, the embryos in the experimental group were treated with 10 µM of forskolin, and the control group embryos were not treated. The evaluation was performed based on blastocyst and hatching rates on D7 and D10. For the evaluation of lipid deposition, blastocysts (D7; n = 10/group) were stained with the Sudan black B. Additionally, 47 genes were analyzed with RTq-PCR. The embryo lipid content (gray intensity) was similar (P > 0.05) between the forskolin (772 ± 4.8) and control (883 ± 5.2) groups. The mRNA transcript levels of GPX1, HAND1, POU5F1 and HP1 were increased in the control group (P = 0.06). The mRNA transcript level of SREBF1 was equal (P = 0.06) in both treatments, and those of AKR1, IFNT2 (P = 0.06) and SCD (P = 0.08) were increased in forskolin-derived embryos when compared with control embryos. There was no difference (P > 0.05) in the blastocyst production rate between the forskolin (37.9%) and control (39.5%) groups. Interestingly, the blastocyst hatching rate at D10 was higher (P = 0.09) in the forskolin group (62.9%) than in the control group (48.4%). |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:59:55Z 2019-10-06T16:59:55Z 2019-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2019.01.004 Livestock Science, v. 221, p. 15-18. 1871-1413 http://hdl.handle.net/11449/190028 10.1016/j.livsci.2019.01.004 2-s2.0-85059784592 3734933152414412 |
url |
http://dx.doi.org/10.1016/j.livsci.2019.01.004 http://hdl.handle.net/11449/190028 |
identifier_str_mv |
Livestock Science, v. 221, p. 15-18. 1871-1413 10.1016/j.livsci.2019.01.004 2-s2.0-85059784592 3734933152414412 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
15-18 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790209987837952 |