Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/are.15923 http://hdl.handle.net/11449/241085 |
Resumo: | Juveniles of Pseudoplatystoma reticulatum (36.25 g and 19.32 cm) were randomly distributed in 16 tanks with 80 L of water. The fish were fed with diets containing probiotic (2%), prebiotic (0.5%), synbiotic (2% probiotic +0.5% prebiotic) and diet without additives (control). After 60 days of feeding, the fish were challenged with inoculation of the Aeromonas hydrophila (0.9 × 106 CFU/mL). Growth performance, physiological, immunological and haematological analyses were evaluated. Fish fed with probiotic, inulin and synbiotic diet showed greater (p = 0.018) weight gain when compared to control fish, and the condition factor was better (p < 0.001) in fish fed with probiotic. Cortisol concentrations increased in all treatments after challenge (p = 0.27), however, after A. hydrophila injection of 6 h, the cortisol levels increased significantly in fish that received control diet. The haematocrit (p < 0.001) and lymphocytes percentages (p < 0.001) decreased after A. hydrophila injection of 3 and 6 h, and neutrophils percentages increased (p < 0.001), without difference among treatments. The not supplemented and supplemented with prebiotic and synbiotic fish groups had decreased haemoglobin concentration (p = 0.01) at after 3 and 6 h. The juvenile catfish fed with probiotic (2% Bacillus subtilis), prebiotic (0.05% inulin) and the synbiotic combination for 60 days had better productive performance, ensuring greater weight gain and growth. The supplemented diets inhibited the cortisol increase when fish are challenged with A. hydrophila, but it does not inhibited the effects of the stress imposed by the bacterial infection. |
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Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatumBacillus subtilisbacterial challengeinulinstresssynbioticJuveniles of Pseudoplatystoma reticulatum (36.25 g and 19.32 cm) were randomly distributed in 16 tanks with 80 L of water. The fish were fed with diets containing probiotic (2%), prebiotic (0.5%), synbiotic (2% probiotic +0.5% prebiotic) and diet without additives (control). After 60 days of feeding, the fish were challenged with inoculation of the Aeromonas hydrophila (0.9 × 106 CFU/mL). Growth performance, physiological, immunological and haematological analyses were evaluated. Fish fed with probiotic, inulin and synbiotic diet showed greater (p = 0.018) weight gain when compared to control fish, and the condition factor was better (p < 0.001) in fish fed with probiotic. Cortisol concentrations increased in all treatments after challenge (p = 0.27), however, after A. hydrophila injection of 6 h, the cortisol levels increased significantly in fish that received control diet. The haematocrit (p < 0.001) and lymphocytes percentages (p < 0.001) decreased after A. hydrophila injection of 3 and 6 h, and neutrophils percentages increased (p < 0.001), without difference among treatments. The not supplemented and supplemented with prebiotic and synbiotic fish groups had decreased haemoglobin concentration (p = 0.01) at after 3 and 6 h. The juvenile catfish fed with probiotic (2% Bacillus subtilis), prebiotic (0.05% inulin) and the synbiotic combination for 60 days had better productive performance, ensuring greater weight gain and growth. The supplemented diets inhibited the cortisol increase when fish are challenged with A. hydrophila, but it does not inhibited the effects of the stress imposed by the bacterial infection.Programa de Pós-Graduação em Zootecnia Universidade Estadual de Mato Grosso do Sul/UEMSPrograma de Pós-Graduação em Ciência Animal Universidade Federal de Mato Grosso do Sul/UFMSPrograma de Pós-Graduação em Ciências Fisiológicas Universidade Federal de São Carlos/UFSCar/Universidade Estadual Paulista UNESP Campus AraraquaraUniversidade Estadual de Mato Grosso do Sul/UEMS Graduação em ZootecniaCentro de Aquicultura da Unesp/CAUNESP Universidade Estadual Paulista/UNESPPrograma de Pós-Graduação em Ciências Fisiológicas Universidade Federal de São Carlos/UFSCar/Universidade Estadual Paulista UNESP Campus AraraquaraCentro de Aquicultura da Unesp/CAUNESP Universidade Estadual Paulista/UNESPUniversidade Estadual de Mato Grosso do Sul (UEMS)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (UNESP)Oliveira, Fúlvia CristinaSoares, Michelly Pereira [UNESP]Oliveira, Beatriz Pacheco NogueiraPilarski, Fabiana [UNESP]Campos, Cristiane Meldau de2023-03-01T20:46:28Z2023-03-01T20:46:28Z2022-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4256-4265http://dx.doi.org/10.1111/are.15923Aquaculture Research, v. 53, n. 12, p. 4256-4265, 2022.1365-21091355-557Xhttp://hdl.handle.net/11449/24108510.1111/are.159232-s2.0-85131154606Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquaculture Researchinfo:eu-repo/semantics/openAccess2023-03-01T20:46:29Zoai:repositorio.unesp.br:11449/241085Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:46:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum |
title |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum |
spellingShingle |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum Oliveira, Fúlvia Cristina Bacillus subtilis bacterial challenge inulin stress synbiotic |
title_short |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum |
title_full |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum |
title_fullStr |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum |
title_full_unstemmed |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum |
title_sort |
Dietary administration of Bacillus subtilis, inulin and its synbiotic combination improves growth and mitigates stress in experimentally infected Pseudoplatystoma reticulatum |
author |
Oliveira, Fúlvia Cristina |
author_facet |
Oliveira, Fúlvia Cristina Soares, Michelly Pereira [UNESP] Oliveira, Beatriz Pacheco Nogueira Pilarski, Fabiana [UNESP] Campos, Cristiane Meldau de |
author_role |
author |
author2 |
Soares, Michelly Pereira [UNESP] Oliveira, Beatriz Pacheco Nogueira Pilarski, Fabiana [UNESP] Campos, Cristiane Meldau de |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Mato Grosso do Sul (UEMS) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Oliveira, Fúlvia Cristina Soares, Michelly Pereira [UNESP] Oliveira, Beatriz Pacheco Nogueira Pilarski, Fabiana [UNESP] Campos, Cristiane Meldau de |
dc.subject.por.fl_str_mv |
Bacillus subtilis bacterial challenge inulin stress synbiotic |
topic |
Bacillus subtilis bacterial challenge inulin stress synbiotic |
description |
Juveniles of Pseudoplatystoma reticulatum (36.25 g and 19.32 cm) were randomly distributed in 16 tanks with 80 L of water. The fish were fed with diets containing probiotic (2%), prebiotic (0.5%), synbiotic (2% probiotic +0.5% prebiotic) and diet without additives (control). After 60 days of feeding, the fish were challenged with inoculation of the Aeromonas hydrophila (0.9 × 106 CFU/mL). Growth performance, physiological, immunological and haematological analyses were evaluated. Fish fed with probiotic, inulin and synbiotic diet showed greater (p = 0.018) weight gain when compared to control fish, and the condition factor was better (p < 0.001) in fish fed with probiotic. Cortisol concentrations increased in all treatments after challenge (p = 0.27), however, after A. hydrophila injection of 6 h, the cortisol levels increased significantly in fish that received control diet. The haematocrit (p < 0.001) and lymphocytes percentages (p < 0.001) decreased after A. hydrophila injection of 3 and 6 h, and neutrophils percentages increased (p < 0.001), without difference among treatments. The not supplemented and supplemented with prebiotic and synbiotic fish groups had decreased haemoglobin concentration (p = 0.01) at after 3 and 6 h. The juvenile catfish fed with probiotic (2% Bacillus subtilis), prebiotic (0.05% inulin) and the synbiotic combination for 60 days had better productive performance, ensuring greater weight gain and growth. The supplemented diets inhibited the cortisol increase when fish are challenged with A. hydrophila, but it does not inhibited the effects of the stress imposed by the bacterial infection. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-08-01 2023-03-01T20:46:28Z 2023-03-01T20:46:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/are.15923 Aquaculture Research, v. 53, n. 12, p. 4256-4265, 2022. 1365-2109 1355-557X http://hdl.handle.net/11449/241085 10.1111/are.15923 2-s2.0-85131154606 |
url |
http://dx.doi.org/10.1111/are.15923 http://hdl.handle.net/11449/241085 |
identifier_str_mv |
Aquaculture Research, v. 53, n. 12, p. 4256-4265, 2022. 1365-2109 1355-557X 10.1111/are.15923 2-s2.0-85131154606 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquaculture Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4256-4265 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789684016873472 |