Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.foodchem.2020.128361 http://hdl.handle.net/11449/206702 |
Resumo: | Shrimp trawling is an important socio-economic activity; however, the bycatch can be problematic to the environment. Thus, the present study investigated potential uses of the bycatch to generate value-added products. The biological activity of the protein hydrolysates obtained from the two most abundant fish species (Micropogonias furnieri and Paralonchurus brasiliensis) was evaluated. Muscle and skin samples of both species were hydrolyzed using two enzymes, Alcalase 2.4 L® or Protamex®. The in vitro antioxidant capacity against peroxyl radicals, DPPH, and sulfhydryl groups were analyzed. Cell viability, Western Blotting, Zymogram, and Real-time PCR analyses were performed. The results showed that the hydrolysates have antioxidant activity and no effect on cell viability at doses lower than 16 mg/mL. In addition, they can modulate extracellular remodelling and intracellular pathways related to cell adhesion. Thus, the hydrolysis of the fish bycatch allows the release of bioactive peptides with potential use in the food industry. |
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Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatchAntioxidant activityCell viabilityDPPHHydrolysisWestern BlottingShrimp trawling is an important socio-economic activity; however, the bycatch can be problematic to the environment. Thus, the present study investigated potential uses of the bycatch to generate value-added products. The biological activity of the protein hydrolysates obtained from the two most abundant fish species (Micropogonias furnieri and Paralonchurus brasiliensis) was evaluated. Muscle and skin samples of both species were hydrolyzed using two enzymes, Alcalase 2.4 L® or Protamex®. The in vitro antioxidant capacity against peroxyl radicals, DPPH, and sulfhydryl groups were analyzed. Cell viability, Western Blotting, Zymogram, and Real-time PCR analyses were performed. The results showed that the hydrolysates have antioxidant activity and no effect on cell viability at doses lower than 16 mg/mL. In addition, they can modulate extracellular remodelling and intracellular pathways related to cell adhesion. Thus, the hydrolysis of the fish bycatch allows the release of bioactive peptides with potential use in the food industry.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Aquaculture Center São Paulo State University (UNESP), Campus JaboticabalMarine Station of Aquaculture Aquaculture postgraduate Oceanography Institute Federal University of Rio Grande (FURG) Rio GrandeInstitute of Biological Sciences (ICB) Federal University of Rio Grande (FURG)Department of Chemistry and Biochemistry Bioscience Institute São Paulo State University (UNESP), Campus BotucatuAquaculture Center São Paulo State University (UNESP), Campus JaboticabalDepartment of Chemistry and Biochemistry Bioscience Institute São Paulo State University (UNESP), Campus BotucatuUniversidade Estadual Paulista (Unesp)Federal University of Rio Grande (FURG) Rio GrandeFederal University of Rio Grande (FURG)Rocha Camargo, Tavani [UNESP]Ramos, PatríciaMonserrat, José M.Prentice, CarlosFernandes, Célio J.C. [UNESP]Zambuzzi, Willian F. [UNESP]Valenti, Wagner C. [UNESP]2021-06-25T10:36:45Z2021-06-25T10:36:45Z2021-04-16info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.foodchem.2020.128361Food Chemistry, v. 342.1873-70720308-8146http://hdl.handle.net/11449/20670210.1016/j.foodchem.2020.1283612-s2.0-85092899393Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Chemistryinfo:eu-repo/semantics/openAccess2024-04-09T15:37:02Zoai:repositorio.unesp.br:11449/206702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:37:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch |
title |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch |
spellingShingle |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch Rocha Camargo, Tavani [UNESP] Antioxidant activity Cell viability DPPH Hydrolysis Western Blotting |
title_short |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch |
title_full |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch |
title_fullStr |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch |
title_full_unstemmed |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch |
title_sort |
Biological activities of the protein hydrolysate obtained from two fishes common in the fisheries bycatch |
author |
Rocha Camargo, Tavani [UNESP] |
author_facet |
Rocha Camargo, Tavani [UNESP] Ramos, Patrícia Monserrat, José M. Prentice, Carlos Fernandes, Célio J.C. [UNESP] Zambuzzi, Willian F. [UNESP] Valenti, Wagner C. [UNESP] |
author_role |
author |
author2 |
Ramos, Patrícia Monserrat, José M. Prentice, Carlos Fernandes, Célio J.C. [UNESP] Zambuzzi, Willian F. [UNESP] Valenti, Wagner C. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Rio Grande (FURG) Rio Grande Federal University of Rio Grande (FURG) |
dc.contributor.author.fl_str_mv |
Rocha Camargo, Tavani [UNESP] Ramos, Patrícia Monserrat, José M. Prentice, Carlos Fernandes, Célio J.C. [UNESP] Zambuzzi, Willian F. [UNESP] Valenti, Wagner C. [UNESP] |
dc.subject.por.fl_str_mv |
Antioxidant activity Cell viability DPPH Hydrolysis Western Blotting |
topic |
Antioxidant activity Cell viability DPPH Hydrolysis Western Blotting |
description |
Shrimp trawling is an important socio-economic activity; however, the bycatch can be problematic to the environment. Thus, the present study investigated potential uses of the bycatch to generate value-added products. The biological activity of the protein hydrolysates obtained from the two most abundant fish species (Micropogonias furnieri and Paralonchurus brasiliensis) was evaluated. Muscle and skin samples of both species were hydrolyzed using two enzymes, Alcalase 2.4 L® or Protamex®. The in vitro antioxidant capacity against peroxyl radicals, DPPH, and sulfhydryl groups were analyzed. Cell viability, Western Blotting, Zymogram, and Real-time PCR analyses were performed. The results showed that the hydrolysates have antioxidant activity and no effect on cell viability at doses lower than 16 mg/mL. In addition, they can modulate extracellular remodelling and intracellular pathways related to cell adhesion. Thus, the hydrolysis of the fish bycatch allows the release of bioactive peptides with potential use in the food industry. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:36:45Z 2021-06-25T10:36:45Z 2021-04-16 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.foodchem.2020.128361 Food Chemistry, v. 342. 1873-7072 0308-8146 http://hdl.handle.net/11449/206702 10.1016/j.foodchem.2020.128361 2-s2.0-85092899393 |
url |
http://dx.doi.org/10.1016/j.foodchem.2020.128361 http://hdl.handle.net/11449/206702 |
identifier_str_mv |
Food Chemistry, v. 342. 1873-7072 0308-8146 10.1016/j.foodchem.2020.128361 2-s2.0-85092899393 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Chemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965342154358784 |