Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/jbm.b.35083 http://hdl.handle.net/11449/241008 |
Resumo: | As the biocompatibility and bioactive potential of repair materials are desired characteristics in dentistry, the tissue response of Bio-C Pulpo, a bioceramic material launched on the marked by Angelus (Brazil), was compared with Biodentine (Septodont, France) and White MTA (WMTA; Angelus, Brazil). In 32 rats, 148 polyethylene tubes filled with Bio-C Pulpo, Biodentine or WMTA, and empty (CG, control group) were implanted into subcutaneous tissues for 7, 15, 30, and 60 days. The capsule thickness, numerical density of inflammatory cells (IC) and fibroblasts (Fb), amount of collagen, immunohistochemistry detection of interleukin-6 (IL-6) and osteocalcin (OCN), von Kossa and analysis under polarized light were performed. Data were subjected to two-way ANOVA followed by Tukey's test (p ≤ 0.05). At 7 and 15 days, the capsules around Bio-C Pulpo were thicker than in WMTA while, at 30 and 60 days, significant differences were not observed among the groups. Although at 7, 15, and 30 days, a greater number of IL-6-immunostained cells was found in Bio-C Pulpo and Biodentine than in WMTA, no significant difference was detected among the groups at 60 days. In all groups, the number of Fb and collagen content increased significantly over time. The capsules around materials exhibited von Kossa-positive and birefringent structures, and OCN-immunostained cells whereas, in the CG, these structures were not observed. Bio-C Pulpo, similarly to Biodentine and WMTA, is biocompatible, allows the connective tissue repair and presents bioactive potential in connective tissue of rats. |
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Repositório Institucional da UNESP |
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Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissuebioactive materialbioceramic materialsimmunohistochemistryinterleukin-6osteocalcinrepair materialstissue reactionAs the biocompatibility and bioactive potential of repair materials are desired characteristics in dentistry, the tissue response of Bio-C Pulpo, a bioceramic material launched on the marked by Angelus (Brazil), was compared with Biodentine (Septodont, France) and White MTA (WMTA; Angelus, Brazil). In 32 rats, 148 polyethylene tubes filled with Bio-C Pulpo, Biodentine or WMTA, and empty (CG, control group) were implanted into subcutaneous tissues for 7, 15, 30, and 60 days. The capsule thickness, numerical density of inflammatory cells (IC) and fibroblasts (Fb), amount of collagen, immunohistochemistry detection of interleukin-6 (IL-6) and osteocalcin (OCN), von Kossa and analysis under polarized light were performed. Data were subjected to two-way ANOVA followed by Tukey's test (p ≤ 0.05). At 7 and 15 days, the capsules around Bio-C Pulpo were thicker than in WMTA while, at 30 and 60 days, significant differences were not observed among the groups. Although at 7, 15, and 30 days, a greater number of IL-6-immunostained cells was found in Bio-C Pulpo and Biodentine than in WMTA, no significant difference was detected among the groups at 60 days. In all groups, the number of Fb and collagen content increased significantly over time. The capsules around materials exhibited von Kossa-positive and birefringent structures, and OCN-immunostained cells whereas, in the CG, these structures were not observed. Bio-C Pulpo, similarly to Biodentine and WMTA, is biocompatible, allows the connective tissue repair and presents bioactive potential in connective tissue of rats.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Restorative Dentistry Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Odontologia Campus de AraraquaraLaboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School São Paulo State University (UNESP)Department of Restorative Dentistry Universidade Estadual Paulista Julio de Mesquita Filho Faculdade de Odontologia Campus de AraraquaraLaboratory of Histology and Embryology Department of Morphology Genetics Orthodontics and Pediatric Dentistry Dental School São Paulo State University (UNESP)CAPES: 001FAPESP: 2019/17739-5Universidade Estadual Paulista (UNESP)Queiroz, Marcela Borsatto [UNESP]Inada, Rafaela Nanami Handa [UNESP]Lopes, Camila Soares [UNESP]Guerreiro-Tanomaru, Juliane Maria [UNESP]Sasso-Cerri, Estela [UNESP]Tanomaru-Filho, Mário [UNESP]Cerri, Paulo Sérgio [UNESP]2023-03-01T20:42:52Z2023-03-01T20:42:52Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2369-2380http://dx.doi.org/10.1002/jbm.b.35083Journal of Biomedical Materials Research - Part B Applied Biomaterials, v. 110, n. 10, p. 2369-2380, 2022.1552-49811552-4973http://hdl.handle.net/11449/24100810.1002/jbm.b.350832-s2.0-85130250906Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biomedical Materials Research - Part B Applied Biomaterialsinfo:eu-repo/semantics/openAccess2023-03-01T20:42:52Zoai:repositorio.unesp.br:11449/241008Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:42:52Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue |
title |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue |
spellingShingle |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue Queiroz, Marcela Borsatto [UNESP] bioactive material bioceramic materials immunohistochemistry interleukin-6 osteocalcin repair materials tissue reaction |
title_short |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue |
title_full |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue |
title_fullStr |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue |
title_full_unstemmed |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue |
title_sort |
Bioactive potential of Bio-C Pulpo is evidenced by presence of birefringent calcite and osteocalcin immunoexpression in the rat subcutaneous tissue |
author |
Queiroz, Marcela Borsatto [UNESP] |
author_facet |
Queiroz, Marcela Borsatto [UNESP] Inada, Rafaela Nanami Handa [UNESP] Lopes, Camila Soares [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
author_role |
author |
author2 |
Inada, Rafaela Nanami Handa [UNESP] Lopes, Camila Soares [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Queiroz, Marcela Borsatto [UNESP] Inada, Rafaela Nanami Handa [UNESP] Lopes, Camila Soares [UNESP] Guerreiro-Tanomaru, Juliane Maria [UNESP] Sasso-Cerri, Estela [UNESP] Tanomaru-Filho, Mário [UNESP] Cerri, Paulo Sérgio [UNESP] |
dc.subject.por.fl_str_mv |
bioactive material bioceramic materials immunohistochemistry interleukin-6 osteocalcin repair materials tissue reaction |
topic |
bioactive material bioceramic materials immunohistochemistry interleukin-6 osteocalcin repair materials tissue reaction |
description |
As the biocompatibility and bioactive potential of repair materials are desired characteristics in dentistry, the tissue response of Bio-C Pulpo, a bioceramic material launched on the marked by Angelus (Brazil), was compared with Biodentine (Septodont, France) and White MTA (WMTA; Angelus, Brazil). In 32 rats, 148 polyethylene tubes filled with Bio-C Pulpo, Biodentine or WMTA, and empty (CG, control group) were implanted into subcutaneous tissues for 7, 15, 30, and 60 days. The capsule thickness, numerical density of inflammatory cells (IC) and fibroblasts (Fb), amount of collagen, immunohistochemistry detection of interleukin-6 (IL-6) and osteocalcin (OCN), von Kossa and analysis under polarized light were performed. Data were subjected to two-way ANOVA followed by Tukey's test (p ≤ 0.05). At 7 and 15 days, the capsules around Bio-C Pulpo were thicker than in WMTA while, at 30 and 60 days, significant differences were not observed among the groups. Although at 7, 15, and 30 days, a greater number of IL-6-immunostained cells was found in Bio-C Pulpo and Biodentine than in WMTA, no significant difference was detected among the groups at 60 days. In all groups, the number of Fb and collagen content increased significantly over time. The capsules around materials exhibited von Kossa-positive and birefringent structures, and OCN-immunostained cells whereas, in the CG, these structures were not observed. Bio-C Pulpo, similarly to Biodentine and WMTA, is biocompatible, allows the connective tissue repair and presents bioactive potential in connective tissue of rats. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-03-01T20:42:52Z 2023-03-01T20:42:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/jbm.b.35083 Journal of Biomedical Materials Research - Part B Applied Biomaterials, v. 110, n. 10, p. 2369-2380, 2022. 1552-4981 1552-4973 http://hdl.handle.net/11449/241008 10.1002/jbm.b.35083 2-s2.0-85130250906 |
url |
http://dx.doi.org/10.1002/jbm.b.35083 http://hdl.handle.net/11449/241008 |
identifier_str_mv |
Journal of Biomedical Materials Research - Part B Applied Biomaterials, v. 110, n. 10, p. 2369-2380, 2022. 1552-4981 1552-4973 10.1002/jbm.b.35083 2-s2.0-85130250906 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biomedical Materials Research - Part B Applied Biomaterials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2369-2380 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965218383593472 |