An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fmicb.2015.01526 http://hdl.handle.net/11449/172527 |
Resumo: | Histoplasma capsulatum is responsible for a human systemic mycosis that primarily affects lung tissue. Macrophages are the major effector cells in humans that respond to the fungus, and the development of respiratory disease depends on the ability of Histoplasma yeast cells to survive and replicate within alveolar macrophages. Therefore, the interaction between macrophages and H. capsulatum is a decisive step in the yeast dissemination into host tissues. Although the role played by components of cell-mediated immunity in the host's defense system and the mechanisms used by the pathogen to evade the host immune response are well understood, knowledge regarding the effects induced by H. capsulatum in host cells at the nuclear level is limited. According to the present findings, H. capsulatum yeast cells display a unique architectural arrangement during the intracellular infection of cultured murine alveolar macrophages, characterized as a formation of aggregates that seem to surround the host cell nucleus, resembling a crown. This extranuclear organization of yeast-aggregates generates damage on the nucleus of the host cell, producing DNA fragmentation and inducing apoptosis, even though the yeast cells are not located inside the nucleus and do not trigger changes in nuclear proteins. The current study highlights a singular intracellular arrangement of H. capsulatum yeast near to the nucleus of infected murine alveolar macrophages that may contribute to the yeast's persistence under intracellular conditions, since this fungal pathogen may display different strategies to prevent elimination by the host's phagocytic mechanisms. |
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An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophagesAlveolar macrophagesHistoplasma capsulatumHost-pathogen interactionsIntracellular arrangementNucleusHistoplasma capsulatum is responsible for a human systemic mycosis that primarily affects lung tissue. Macrophages are the major effector cells in humans that respond to the fungus, and the development of respiratory disease depends on the ability of Histoplasma yeast cells to survive and replicate within alveolar macrophages. Therefore, the interaction between macrophages and H. capsulatum is a decisive step in the yeast dissemination into host tissues. Although the role played by components of cell-mediated immunity in the host's defense system and the mechanisms used by the pathogen to evade the host immune response are well understood, knowledge regarding the effects induced by H. capsulatum in host cells at the nuclear level is limited. According to the present findings, H. capsulatum yeast cells display a unique architectural arrangement during the intracellular infection of cultured murine alveolar macrophages, characterized as a formation of aggregates that seem to surround the host cell nucleus, resembling a crown. This extranuclear organization of yeast-aggregates generates damage on the nucleus of the host cell, producing DNA fragmentation and inducing apoptosis, even though the yeast cells are not located inside the nucleus and do not trigger changes in nuclear proteins. The current study highlights a singular intracellular arrangement of H. capsulatum yeast near to the nucleus of infected murine alveolar macrophages that may contribute to the yeast's persistence under intracellular conditions, since this fungal pathogen may display different strategies to prevent elimination by the host's phagocytic mechanisms.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Ciências Farmacêuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Micologia Clínica, Campus AraraquaraDepartamento de Microbiologia y Parasitologia Facultad de Medicina Universidad Nacional Autónoma de MéxicoFaculdade de Ciências Farmacêuticas UNESP - Univ Estadual Paulista Departamento de Análises Clínicas Laboratório de Micologia Clínica, Campus AraraquaraCNPq: CNPq 480316/2012-0Universidade Estadual Paulista (Unesp)Universidad Nacional Autónoma de MéxicoPitangui, Nayla de Souza [UNESP]Sardi, Janaina de Cássia Orlandi [UNESP]Voltan, Aline R. [UNESP]dos Santos, Claudia T. [UNESP]da Silva, Julhiany de Fátima [UNESP]da Silva, Rosangela A.M. [UNESP]Souza, Felipe O. [UNESP]Soares, Christiane P. [UNESP]Rodríguez-Arellanes, GabrielaTaylor, Maria LuciaMendes-Giannini, Maria J.S. [UNESP]Fusco-Almeida, Ana M. [UNESP]2018-12-11T17:00:48Z2018-12-11T17:00:48Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.3389/fmicb.2015.01526Frontiers in Microbiology, v. 6, n. JAN, 2016.1664-302Xhttp://hdl.handle.net/11449/17252710.3389/fmicb.2015.015262-s2.0-849579916302-s2.0-84957991630.pdf17680252903736690000-0003-1740-7360Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Microbiologyinfo:eu-repo/semantics/openAccess2023-10-22T06:08:39Zoai:repositorio.unesp.br:11449/172527Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-22T06:08:39Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
title |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
spellingShingle |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages Pitangui, Nayla de Souza [UNESP] Alveolar macrophages Histoplasma capsulatum Host-pathogen interactions Intracellular arrangement Nucleus |
title_short |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
title_full |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
title_fullStr |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
title_full_unstemmed |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
title_sort |
An intracellular arrangement of Histoplasma capsulatum yeast-aggregates generates nuclear damage to the cultured murine alveolar macrophages |
author |
Pitangui, Nayla de Souza [UNESP] |
author_facet |
Pitangui, Nayla de Souza [UNESP] Sardi, Janaina de Cássia Orlandi [UNESP] Voltan, Aline R. [UNESP] dos Santos, Claudia T. [UNESP] da Silva, Julhiany de Fátima [UNESP] da Silva, Rosangela A.M. [UNESP] Souza, Felipe O. [UNESP] Soares, Christiane P. [UNESP] Rodríguez-Arellanes, Gabriela Taylor, Maria Lucia Mendes-Giannini, Maria J.S. [UNESP] Fusco-Almeida, Ana M. [UNESP] |
author_role |
author |
author2 |
Sardi, Janaina de Cássia Orlandi [UNESP] Voltan, Aline R. [UNESP] dos Santos, Claudia T. [UNESP] da Silva, Julhiany de Fátima [UNESP] da Silva, Rosangela A.M. [UNESP] Souza, Felipe O. [UNESP] Soares, Christiane P. [UNESP] Rodríguez-Arellanes, Gabriela Taylor, Maria Lucia Mendes-Giannini, Maria J.S. [UNESP] Fusco-Almeida, Ana M. [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidad Nacional Autónoma de México |
dc.contributor.author.fl_str_mv |
Pitangui, Nayla de Souza [UNESP] Sardi, Janaina de Cássia Orlandi [UNESP] Voltan, Aline R. [UNESP] dos Santos, Claudia T. [UNESP] da Silva, Julhiany de Fátima [UNESP] da Silva, Rosangela A.M. [UNESP] Souza, Felipe O. [UNESP] Soares, Christiane P. [UNESP] Rodríguez-Arellanes, Gabriela Taylor, Maria Lucia Mendes-Giannini, Maria J.S. [UNESP] Fusco-Almeida, Ana M. [UNESP] |
dc.subject.por.fl_str_mv |
Alveolar macrophages Histoplasma capsulatum Host-pathogen interactions Intracellular arrangement Nucleus |
topic |
Alveolar macrophages Histoplasma capsulatum Host-pathogen interactions Intracellular arrangement Nucleus |
description |
Histoplasma capsulatum is responsible for a human systemic mycosis that primarily affects lung tissue. Macrophages are the major effector cells in humans that respond to the fungus, and the development of respiratory disease depends on the ability of Histoplasma yeast cells to survive and replicate within alveolar macrophages. Therefore, the interaction between macrophages and H. capsulatum is a decisive step in the yeast dissemination into host tissues. Although the role played by components of cell-mediated immunity in the host's defense system and the mechanisms used by the pathogen to evade the host immune response are well understood, knowledge regarding the effects induced by H. capsulatum in host cells at the nuclear level is limited. According to the present findings, H. capsulatum yeast cells display a unique architectural arrangement during the intracellular infection of cultured murine alveolar macrophages, characterized as a formation of aggregates that seem to surround the host cell nucleus, resembling a crown. This extranuclear organization of yeast-aggregates generates damage on the nucleus of the host cell, producing DNA fragmentation and inducing apoptosis, even though the yeast cells are not located inside the nucleus and do not trigger changes in nuclear proteins. The current study highlights a singular intracellular arrangement of H. capsulatum yeast near to the nucleus of infected murine alveolar macrophages that may contribute to the yeast's persistence under intracellular conditions, since this fungal pathogen may display different strategies to prevent elimination by the host's phagocytic mechanisms. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:00:48Z 2018-12-11T17:00:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fmicb.2015.01526 Frontiers in Microbiology, v. 6, n. JAN, 2016. 1664-302X http://hdl.handle.net/11449/172527 10.3389/fmicb.2015.01526 2-s2.0-84957991630 2-s2.0-84957991630.pdf 1768025290373669 0000-0003-1740-7360 |
url |
http://dx.doi.org/10.3389/fmicb.2015.01526 http://hdl.handle.net/11449/172527 |
identifier_str_mv |
Frontiers in Microbiology, v. 6, n. JAN, 2016. 1664-302X 10.3389/fmicb.2015.01526 2-s2.0-84957991630 2-s2.0-84957991630.pdf 1768025290373669 0000-0003-1740-7360 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964657061986304 |