Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.anifeedsci.2022.115253 http://hdl.handle.net/11449/234245 |
Resumo: | Food security and climate change concerns are pressing livestock production industry to seek sustainable practices to increase productivity while reducing the intensity of enteric methane (CH4) emissions. In this scenario, the use of enzyme products in ruminant nutrition may be an important alternative to meet future increased demands for animal products with reduced environmental impacts. The objective of this study was to evaluate the effects of treating forage with a fibrolytic enzymes extract (FEE), produced by Trichoderma reesei, on animal performance, blood biochemical parameters, nutrient digestibility, ruminal fermentation, and enteric CH4 production of growing lambs. Nineteen Santa Inês growing lambs were divided into two groups: control (CTL) and enzyme (ENZ). The animals were fed with 700 g/kg of tifton-85 hay and 300 g/kg (as fed basis) of concentrate mixture for 73 days. The FEE was manually applied 24 h before each feed supply. Initially, animals were kept in individual pens for the growth performance trial (15 days of adaptation plus 45 for data collection), then were taken to metabolic cages (seven days) and to gas measurement chambers (six days) for the metabolism assay and determination of CH4 production, respectively. No effects (P > 0.05) were observed on dry matter intake (DMI), average daily gain (ADG), and feed conversion ratio. However, ENZ animals showed higher proportion of body weight gain (PBWG) when considering the entire growth performance period (P < 0.05). Analysis of blood parameters indicated that FEE did not impair animals’ health. Lambs fed forages treated with FEE showed 12% higher acid detergent fiber (ADF) digestibility (P < 0.05) and 20% lower enteric CH4 yield (i.e. per kg of digestible organic matter intake) (P < 0.05). Microbial protein synthesis, pH, ammonia nitrogen, short-chain fatty acids concentration and protozoa population were not affected by the addition of FEE (P > 0.05). The results indicated that FEE can be used in diets of lambs, leading to higher PBWG, improving ADF digestibility and reducing CH4 yield, suggesting its potential suitability as a strategy to increase animal productivity and contribute to the mitigation of enteric CH4 production, especially when animals are fed poor-quality forages. |
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Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambsCellulaseCH4 mitigationGrowth performanceRuminantSustainabilityXylanaseFood security and climate change concerns are pressing livestock production industry to seek sustainable practices to increase productivity while reducing the intensity of enteric methane (CH4) emissions. In this scenario, the use of enzyme products in ruminant nutrition may be an important alternative to meet future increased demands for animal products with reduced environmental impacts. The objective of this study was to evaluate the effects of treating forage with a fibrolytic enzymes extract (FEE), produced by Trichoderma reesei, on animal performance, blood biochemical parameters, nutrient digestibility, ruminal fermentation, and enteric CH4 production of growing lambs. Nineteen Santa Inês growing lambs were divided into two groups: control (CTL) and enzyme (ENZ). The animals were fed with 700 g/kg of tifton-85 hay and 300 g/kg (as fed basis) of concentrate mixture for 73 days. The FEE was manually applied 24 h before each feed supply. Initially, animals were kept in individual pens for the growth performance trial (15 days of adaptation plus 45 for data collection), then were taken to metabolic cages (seven days) and to gas measurement chambers (six days) for the metabolism assay and determination of CH4 production, respectively. No effects (P > 0.05) were observed on dry matter intake (DMI), average daily gain (ADG), and feed conversion ratio. However, ENZ animals showed higher proportion of body weight gain (PBWG) when considering the entire growth performance period (P < 0.05). Analysis of blood parameters indicated that FEE did not impair animals’ health. Lambs fed forages treated with FEE showed 12% higher acid detergent fiber (ADF) digestibility (P < 0.05) and 20% lower enteric CH4 yield (i.e. per kg of digestible organic matter intake) (P < 0.05). Microbial protein synthesis, pH, ammonia nitrogen, short-chain fatty acids concentration and protozoa population were not affected by the addition of FEE (P > 0.05). The results indicated that FEE can be used in diets of lambs, leading to higher PBWG, improving ADF digestibility and reducing CH4 yield, suggesting its potential suitability as a strategy to increase animal productivity and contribute to the mitigation of enteric CH4 production, especially when animals are fed poor-quality forages.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade de São Paulo Centro de Energia Nuclear na Agricultura Laboratório de Nutrição AnimalUniversidade Estadual Paulista Instituto de Pesquisas em Bioenergia Unidade de BioprocessosUniversidade do Oeste Paulista Departamento de Ciências Funcionais Laboratório de BiofísicaInstituto Federal Goiano de Educação Ciência e Tecnologia Campus Iporá Laboratório de Produção AnimalUniversidade Estadual Paulista Instituto de Pesquisas em Bioenergia Unidade de BioprocessosCNPq: 152612/2016-5CAPES: CAPES/PROEX - 0227080Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Laboratório de BiofísicaLaboratório de Produção AnimalSakita, Gabriel ZanutoLima, Paulo de Mello TavaresAbdalla Filho, Adibe LuizBompadre, Thiago Francisco VentosoOvani, Vagner S.Chaves, Camila de Miranda e SilvaBizzuti, Beatriz ElisaCosta, Wilian dos Santos daPaim, Tiago do PradoCampioni, Tania Sila [UNESP]Oliva Neto, Pedro de [UNESP]Bremer-Neto, HermannLouvandini, HelderAbdalla, Adibe Luiz2022-05-01T15:13:38Z2022-05-01T15:13:38Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.anifeedsci.2022.115253Animal Feed Science and Technology, v. 286.0377-8401http://hdl.handle.net/11449/23424510.1016/j.anifeedsci.2022.1152532-s2.0-85126057532Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimal Feed Science and Technologyinfo:eu-repo/semantics/openAccess2022-05-01T15:13:38Zoai:repositorio.unesp.br:11449/234245Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T15:13:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs |
title |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs |
spellingShingle |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs Sakita, Gabriel Zanuto Cellulase CH4 mitigation Growth performance Ruminant Sustainability Xylanase |
title_short |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs |
title_full |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs |
title_fullStr |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs |
title_full_unstemmed |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs |
title_sort |
Treating tropical grass with fibrolytic enzymes from the fungus Trichoderma reesei: Effects on animal performance, digestibility and enteric methane emissions of growing lambs |
author |
Sakita, Gabriel Zanuto |
author_facet |
Sakita, Gabriel Zanuto Lima, Paulo de Mello Tavares Abdalla Filho, Adibe Luiz Bompadre, Thiago Francisco Ventoso Ovani, Vagner S. Chaves, Camila de Miranda e Silva Bizzuti, Beatriz Elisa Costa, Wilian dos Santos da Paim, Tiago do Prado Campioni, Tania Sila [UNESP] Oliva Neto, Pedro de [UNESP] Bremer-Neto, Hermann Louvandini, Helder Abdalla, Adibe Luiz |
author_role |
author |
author2 |
Lima, Paulo de Mello Tavares Abdalla Filho, Adibe Luiz Bompadre, Thiago Francisco Ventoso Ovani, Vagner S. Chaves, Camila de Miranda e Silva Bizzuti, Beatriz Elisa Costa, Wilian dos Santos da Paim, Tiago do Prado Campioni, Tania Sila [UNESP] Oliva Neto, Pedro de [UNESP] Bremer-Neto, Hermann Louvandini, Helder Abdalla, Adibe Luiz |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Laboratório de Biofísica Laboratório de Produção Animal |
dc.contributor.author.fl_str_mv |
Sakita, Gabriel Zanuto Lima, Paulo de Mello Tavares Abdalla Filho, Adibe Luiz Bompadre, Thiago Francisco Ventoso Ovani, Vagner S. Chaves, Camila de Miranda e Silva Bizzuti, Beatriz Elisa Costa, Wilian dos Santos da Paim, Tiago do Prado Campioni, Tania Sila [UNESP] Oliva Neto, Pedro de [UNESP] Bremer-Neto, Hermann Louvandini, Helder Abdalla, Adibe Luiz |
dc.subject.por.fl_str_mv |
Cellulase CH4 mitigation Growth performance Ruminant Sustainability Xylanase |
topic |
Cellulase CH4 mitigation Growth performance Ruminant Sustainability Xylanase |
description |
Food security and climate change concerns are pressing livestock production industry to seek sustainable practices to increase productivity while reducing the intensity of enteric methane (CH4) emissions. In this scenario, the use of enzyme products in ruminant nutrition may be an important alternative to meet future increased demands for animal products with reduced environmental impacts. The objective of this study was to evaluate the effects of treating forage with a fibrolytic enzymes extract (FEE), produced by Trichoderma reesei, on animal performance, blood biochemical parameters, nutrient digestibility, ruminal fermentation, and enteric CH4 production of growing lambs. Nineteen Santa Inês growing lambs were divided into two groups: control (CTL) and enzyme (ENZ). The animals were fed with 700 g/kg of tifton-85 hay and 300 g/kg (as fed basis) of concentrate mixture for 73 days. The FEE was manually applied 24 h before each feed supply. Initially, animals were kept in individual pens for the growth performance trial (15 days of adaptation plus 45 for data collection), then were taken to metabolic cages (seven days) and to gas measurement chambers (six days) for the metabolism assay and determination of CH4 production, respectively. No effects (P > 0.05) were observed on dry matter intake (DMI), average daily gain (ADG), and feed conversion ratio. However, ENZ animals showed higher proportion of body weight gain (PBWG) when considering the entire growth performance period (P < 0.05). Analysis of blood parameters indicated that FEE did not impair animals’ health. Lambs fed forages treated with FEE showed 12% higher acid detergent fiber (ADF) digestibility (P < 0.05) and 20% lower enteric CH4 yield (i.e. per kg of digestible organic matter intake) (P < 0.05). Microbial protein synthesis, pH, ammonia nitrogen, short-chain fatty acids concentration and protozoa population were not affected by the addition of FEE (P > 0.05). The results indicated that FEE can be used in diets of lambs, leading to higher PBWG, improving ADF digestibility and reducing CH4 yield, suggesting its potential suitability as a strategy to increase animal productivity and contribute to the mitigation of enteric CH4 production, especially when animals are fed poor-quality forages. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T15:13:38Z 2022-05-01T15:13:38Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.anifeedsci.2022.115253 Animal Feed Science and Technology, v. 286. 0377-8401 http://hdl.handle.net/11449/234245 10.1016/j.anifeedsci.2022.115253 2-s2.0-85126057532 |
url |
http://dx.doi.org/10.1016/j.anifeedsci.2022.115253 http://hdl.handle.net/11449/234245 |
identifier_str_mv |
Animal Feed Science and Technology, v. 286. 0377-8401 10.1016/j.anifeedsci.2022.115253 2-s2.0-85126057532 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animal Feed Science and Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789794680438784 |