Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina
Main Author: | |
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Publication Date: | 2015 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/S1984-29612015060 http://hdl.handle.net/11449/177514 |
Summary: | This study aimed to: express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain, test this protein as an antigen in serological assays, and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three different strains of Escherichia coli. We used 29 sera samples from Leishmune-vaccinated dogs, 482 sera samples from dogs from endemic areas (positive controls), and 170 sera samples from dogs from non-endemic areas (negative controls) in ELISA tests using soluble Leishmania antigen (SLA) and His-A2 as antigen. Expressed proteins showed, by western blotting, the expression of an 11 KDa protein. Sixtythree percent (303/482) of the samples from endemic areas were positive by ELISA His-A2, whereas 93.1% (27/29) of Leishmune®-vaccinated animals were negative by His-A2-ELISA. Anti-A2 antibodies from mice inoculated with the A2 protein were detected in slides containing amastigote forms, but not in slides containing promastigote forms. The A2 recombinant protein from L. chagasi may be a useful tool in the diagnosis of CVL, and further tests regarding the infection stage and the specie of parasite at which the dogs are sampled should provide a better understanding of our results. |
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Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral CaninaExpression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in Canine Visceral Leishmaniasis serological testA2DiagnosisELISAEscherichia coliLeishmania infantumThis study aimed to: express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain, test this protein as an antigen in serological assays, and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three different strains of Escherichia coli. We used 29 sera samples from Leishmune-vaccinated dogs, 482 sera samples from dogs from endemic areas (positive controls), and 170 sera samples from dogs from non-endemic areas (negative controls) in ELISA tests using soluble Leishmania antigen (SLA) and His-A2 as antigen. Expressed proteins showed, by western blotting, the expression of an 11 KDa protein. Sixtythree percent (303/482) of the samples from endemic areas were positive by ELISA His-A2, whereas 93.1% (27/29) of Leishmune®-vaccinated animals were negative by His-A2-ELISA. Anti-A2 antibodies from mice inoculated with the A2 protein were detected in slides containing amastigote forms, but not in slides containing promastigote forms. The A2 recombinant protein from L. chagasi may be a useful tool in the diagnosis of CVL, and further tests regarding the infection stage and the specie of parasite at which the dogs are sampled should provide a better understanding of our results.Laboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Universidade Estadual Paulista – UNESPDepartamento de Zootecnia, Universidade de São Paulo – USPLaboratório de Imunoparasitologia, Departamento de Patologia Veterinária, Universidade Estadual Paulista – UNESPUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Jusi, Márcia Mariza Gomes [UNESP]Oliveira, Trícia Maria Ferreira de SousaNakaghi, Andréa Cristina HigaAndré, Marcos Rogério [UNESP]Machado, Rosangela Zacarias [UNESP]2018-12-11T17:25:48Z2018-12-11T17:25:48Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article309-316application/pdfhttp://dx.doi.org/10.1590/S1984-29612015060Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 3, p. 309-316, 2015.0103-846Xhttp://hdl.handle.net/11449/17751410.1590/S1984-29612015060S1984-296120150003003092-s2.0-84942944294S1984-29612015000300309.pdf9139899895580513Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Parasitologia Veterinariainfo:eu-repo/semantics/openAccess2023-12-25T06:20:07Zoai:repositorio.unesp.br:11449/177514Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-25T06:20:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina Expression of a recombinant protein, A2 family, from Leishmania infantum (Jaboticabal strain) and its evaluation in Canine Visceral Leishmaniasis serological test |
title |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina |
spellingShingle |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina Jusi, Márcia Mariza Gomes [UNESP] A2 Diagnosis ELISA Escherichia coli Leishmania infantum |
title_short |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina |
title_full |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina |
title_fullStr |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina |
title_full_unstemmed |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina |
title_sort |
Expressão de uma proteína recombinante, da família A2, de Leishmania infantum (amostra Jaboticabal) e sua avaliação no teste sorológico da Leishmaniose Visceral Canina |
author |
Jusi, Márcia Mariza Gomes [UNESP] |
author_facet |
Jusi, Márcia Mariza Gomes [UNESP] Oliveira, Trícia Maria Ferreira de Sousa Nakaghi, Andréa Cristina Higa André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] |
author_role |
author |
author2 |
Oliveira, Trícia Maria Ferreira de Sousa Nakaghi, Andréa Cristina Higa André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Jusi, Márcia Mariza Gomes [UNESP] Oliveira, Trícia Maria Ferreira de Sousa Nakaghi, Andréa Cristina Higa André, Marcos Rogério [UNESP] Machado, Rosangela Zacarias [UNESP] |
dc.subject.por.fl_str_mv |
A2 Diagnosis ELISA Escherichia coli Leishmania infantum |
topic |
A2 Diagnosis ELISA Escherichia coli Leishmania infantum |
description |
This study aimed to: express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain, test this protein as an antigen in serological assays, and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi was expressed in three different strains of Escherichia coli. We used 29 sera samples from Leishmune-vaccinated dogs, 482 sera samples from dogs from endemic areas (positive controls), and 170 sera samples from dogs from non-endemic areas (negative controls) in ELISA tests using soluble Leishmania antigen (SLA) and His-A2 as antigen. Expressed proteins showed, by western blotting, the expression of an 11 KDa protein. Sixtythree percent (303/482) of the samples from endemic areas were positive by ELISA His-A2, whereas 93.1% (27/29) of Leishmune®-vaccinated animals were negative by His-A2-ELISA. Anti-A2 antibodies from mice inoculated with the A2 protein were detected in slides containing amastigote forms, but not in slides containing promastigote forms. The A2 recombinant protein from L. chagasi may be a useful tool in the diagnosis of CVL, and further tests regarding the infection stage and the specie of parasite at which the dogs are sampled should provide a better understanding of our results. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-12-11T17:25:48Z 2018-12-11T17:25:48Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1984-29612015060 Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 3, p. 309-316, 2015. 0103-846X http://hdl.handle.net/11449/177514 10.1590/S1984-29612015060 S1984-29612015000300309 2-s2.0-84942944294 S1984-29612015000300309.pdf 9139899895580513 |
url |
http://dx.doi.org/10.1590/S1984-29612015060 http://hdl.handle.net/11449/177514 |
identifier_str_mv |
Revista Brasileira de Parasitologia Veterinaria, v. 24, n. 3, p. 309-316, 2015. 0103-846X 10.1590/S1984-29612015060 S1984-29612015000300309 2-s2.0-84942944294 S1984-29612015000300309.pdf 9139899895580513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Parasitologia Veterinaria |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
309-316 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799965400887197696 |