Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/08927014.2019.1686485 http://hdl.handle.net/11449/201343 |
Resumo: | Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against Candida, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis. For the in vivo study, Galleria mellonella was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the Candida species tested. Furthermore, injection of S-PRG eluate into G. mellonella was not toxic to the larvae and protected G. mellonella against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by C. albicans, C. glabrata, C. krusei, and C. tropicalis and exerted protective effects on G. mellonella against experimental candidiasis in vivo. |
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spelling |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infectionbiofilmsCandida sppGalleria mellonellaS-PRG fillerSurface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against Candida, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis. For the in vivo study, Galleria mellonella was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the Candida species tested. Furthermore, injection of S-PRG eluate into G. mellonella was not toxic to the larvae and protected G. mellonella against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by C. albicans, C. glabrata, C. krusei, and C. tropicalis and exerted protective effects on G. mellonella against experimental candidiasis in vivo.Department of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP - Univ Estadual PaulistaDepartment of Research SHOFU Inc.Department of Biosciences and Oral Diagnosis Institute of Science and Technology UNESP - Univ Estadual PaulistaUniversidade Estadual Paulista (Unesp)SHOFU Inc.Rossoni, Rodnei Dennis [UNESP]de Barros, Patrícia Pimentel [UNESP]Lopes, Lucas Alexandre das Chagas [UNESP]Ribeiro, Felipe Camargo [UNESP]Nakatsuka, ToshiyukiKasaba, HidetoJunqueira, Juliana Campos [UNESP]2020-12-12T02:30:07Z2020-12-12T02:30:07Z2019-10-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article997-1006http://dx.doi.org/10.1080/08927014.2019.1686485Biofouling, v. 35, n. 9, p. 997-1006, 2019.1029-24540892-7014http://hdl.handle.net/11449/20134310.1080/08927014.2019.16864852-s2.0-85075434413Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiofoulinginfo:eu-repo/semantics/openAccess2021-10-22T17:20:00Zoai:repositorio.unesp.br:11449/201343Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T17:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection |
title |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection |
spellingShingle |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection Rossoni, Rodnei Dennis [UNESP] biofilms Candida spp Galleria mellonella S-PRG filler |
title_short |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection |
title_full |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection |
title_fullStr |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection |
title_full_unstemmed |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection |
title_sort |
Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on Candida spp.: antifungal activity, anti-biofilm properties, and protective effects on Galleria mellonella against C. albicans infection |
author |
Rossoni, Rodnei Dennis [UNESP] |
author_facet |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] Lopes, Lucas Alexandre das Chagas [UNESP] Ribeiro, Felipe Camargo [UNESP] Nakatsuka, Toshiyuki Kasaba, Hideto Junqueira, Juliana Campos [UNESP] |
author_role |
author |
author2 |
de Barros, Patrícia Pimentel [UNESP] Lopes, Lucas Alexandre das Chagas [UNESP] Ribeiro, Felipe Camargo [UNESP] Nakatsuka, Toshiyuki Kasaba, Hideto Junqueira, Juliana Campos [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) SHOFU Inc. |
dc.contributor.author.fl_str_mv |
Rossoni, Rodnei Dennis [UNESP] de Barros, Patrícia Pimentel [UNESP] Lopes, Lucas Alexandre das Chagas [UNESP] Ribeiro, Felipe Camargo [UNESP] Nakatsuka, Toshiyuki Kasaba, Hideto Junqueira, Juliana Campos [UNESP] |
dc.subject.por.fl_str_mv |
biofilms Candida spp Galleria mellonella S-PRG filler |
topic |
biofilms Candida spp Galleria mellonella S-PRG filler |
description |
Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against Candida, the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis. For the in vivo study, Galleria mellonella was used as a model to evaluate the effects of S-PRG on toxicity, hemocyte counts and candidiasis. The MIC of S-PRG ranged from 5 to 40% (v/v). S-PRG eluate exhibited anti-biofilm activity for all the Candida species tested. Furthermore, injection of S-PRG eluate into G. mellonella was not toxic to the larvae and protected G. mellonella against experimental candidiasis. In addition, S-PRG eluate inhibited biofilm formation by C. albicans, C. glabrata, C. krusei, and C. tropicalis and exerted protective effects on G. mellonella against experimental candidiasis in vivo. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-21 2020-12-12T02:30:07Z 2020-12-12T02:30:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/08927014.2019.1686485 Biofouling, v. 35, n. 9, p. 997-1006, 2019. 1029-2454 0892-7014 http://hdl.handle.net/11449/201343 10.1080/08927014.2019.1686485 2-s2.0-85075434413 |
url |
http://dx.doi.org/10.1080/08927014.2019.1686485 http://hdl.handle.net/11449/201343 |
identifier_str_mv |
Biofouling, v. 35, n. 9, p. 997-1006, 2019. 1029-2454 0892-7014 10.1080/08927014.2019.1686485 2-s2.0-85075434413 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biofouling |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
997-1006 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965170127077376 |