Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pnpbp.2017.10.018 http://hdl.handle.net/11449/175462 |
Resumo: | Exposure to elevated concentrations of CO2 or hypoxia has been widely used in psychiatric research as a panic provoking stimulus. However, the use of these respiratory challenges to model panic-like responses in experimental animals has been less straightforward. Little data is available, from behavioral and endocrine perspectives, to support the conclusion that a marked aversive situation, such as that experienced during panic attacks, was evoked in these animals. We here compared the behavioral responses of male CB57BL/6 mice during exposure to 20% CO2 or 7% O2 and its consequence on plasma levels of corticosterone. We also evaluated whether clinically-effective panicolytic drugs affect the behavioral responses expressed during CO2 exposure. The results showed that whereas hypoxia caused a marked reduction in locomotion, inhalation of CO2-enriched air evoked an active escape response, characterized by bouts of upward leaps directed to the border of the experimental cage, interpreted as escape attempts. Corticosterone levels were increased 30 min after either of the respiratory challenges used, but it was higher in the hypoxia group. Chronic (21 days), but not acute, treatment with fluoxetine or imipramine (5, 10 or 15 mg/kg) or a single injection of alprazolam (0.025, 0.05 or 0.1 mg/kg), but not of the anxiolytic diazepam (0.025, 0.05 or 0.1 and 1 mg/kg) reduced the number of escape attempts, indicating a panicolytic-like effect. Altogether, the results suggest that whereas hypoxia increased anxiety, exposure to 20% CO2 evoked a panic-like state. The latter condition/test protocol seems to be a simple and validated model for studying in mice pathophysiological mechanisms and the screening of novel drugs for panic disorder. |
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Panic-like escape response elicited in mice by exposure to CO2, but not hypoxiaCO2-exposureCorticosteroneEscapeHypoxiaPanicExposure to elevated concentrations of CO2 or hypoxia has been widely used in psychiatric research as a panic provoking stimulus. However, the use of these respiratory challenges to model panic-like responses in experimental animals has been less straightforward. Little data is available, from behavioral and endocrine perspectives, to support the conclusion that a marked aversive situation, such as that experienced during panic attacks, was evoked in these animals. We here compared the behavioral responses of male CB57BL/6 mice during exposure to 20% CO2 or 7% O2 and its consequence on plasma levels of corticosterone. We also evaluated whether clinically-effective panicolytic drugs affect the behavioral responses expressed during CO2 exposure. The results showed that whereas hypoxia caused a marked reduction in locomotion, inhalation of CO2-enriched air evoked an active escape response, characterized by bouts of upward leaps directed to the border of the experimental cage, interpreted as escape attempts. Corticosterone levels were increased 30 min after either of the respiratory challenges used, but it was higher in the hypoxia group. Chronic (21 days), but not acute, treatment with fluoxetine or imipramine (5, 10 or 15 mg/kg) or a single injection of alprazolam (0.025, 0.05 or 0.1 mg/kg), but not of the anxiolytic diazepam (0.025, 0.05 or 0.1 and 1 mg/kg) reduced the number of escape attempts, indicating a panicolytic-like effect. Altogether, the results suggest that whereas hypoxia increased anxiety, exposure to 20% CO2 evoked a panic-like state. The latter condition/test protocol seems to be a simple and validated model for studying in mice pathophysiological mechanisms and the screening of novel drugs for panic disorder.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Pharmacology School of Medicine of Ribeirão Preto University of São Paulo, Av. Bandeirantes, 3900Department of Animal Morphology and Physiology São Paulo State University UNESP FCAVDepartment of Physiology School of Medicine of Ribeirao Preto University of São PauloDepartment of Animal Morphology and Physiology São Paulo State University UNESP FCAVFAPESP: 12/17626-7CAPES: 1281474CNPq: 466796/2014-5Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Spiacci, AiltonVilela-Costa, Heloisa H.Sant'Ana, Ana BeatrizFernandes, Gabriel GrippFrias, Alana Tercinoda Silva, Glauber S. Ferreira [UNESP]Antunes-Rodrigues, JoséZangrossi, Hélio2018-12-11T17:15:56Z2018-12-11T17:15:56Z2018-02-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article178-186application/pdfhttp://dx.doi.org/10.1016/j.pnpbp.2017.10.018Progress in Neuro-Psychopharmacology and Biological Psychiatry, v. 81, p. 178-186.1878-42160278-5846http://hdl.handle.net/11449/17546210.1016/j.pnpbp.2017.10.0182-s2.0-850334094732-s2.0-85033409473.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProgress in Neuro-Psychopharmacology and Biological Psychiatry1,714info:eu-repo/semantics/openAccess2024-01-17T06:28:59Zoai:repositorio.unesp.br:11449/175462Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-17T06:28:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia |
title |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia |
spellingShingle |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia Spiacci, Ailton CO2-exposure Corticosterone Escape Hypoxia Panic |
title_short |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia |
title_full |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia |
title_fullStr |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia |
title_full_unstemmed |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia |
title_sort |
Panic-like escape response elicited in mice by exposure to CO2, but not hypoxia |
author |
Spiacci, Ailton |
author_facet |
Spiacci, Ailton Vilela-Costa, Heloisa H. Sant'Ana, Ana Beatriz Fernandes, Gabriel Gripp Frias, Alana Tercino da Silva, Glauber S. Ferreira [UNESP] Antunes-Rodrigues, José Zangrossi, Hélio |
author_role |
author |
author2 |
Vilela-Costa, Heloisa H. Sant'Ana, Ana Beatriz Fernandes, Gabriel Gripp Frias, Alana Tercino da Silva, Glauber S. Ferreira [UNESP] Antunes-Rodrigues, José Zangrossi, Hélio |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Spiacci, Ailton Vilela-Costa, Heloisa H. Sant'Ana, Ana Beatriz Fernandes, Gabriel Gripp Frias, Alana Tercino da Silva, Glauber S. Ferreira [UNESP] Antunes-Rodrigues, José Zangrossi, Hélio |
dc.subject.por.fl_str_mv |
CO2-exposure Corticosterone Escape Hypoxia Panic |
topic |
CO2-exposure Corticosterone Escape Hypoxia Panic |
description |
Exposure to elevated concentrations of CO2 or hypoxia has been widely used in psychiatric research as a panic provoking stimulus. However, the use of these respiratory challenges to model panic-like responses in experimental animals has been less straightforward. Little data is available, from behavioral and endocrine perspectives, to support the conclusion that a marked aversive situation, such as that experienced during panic attacks, was evoked in these animals. We here compared the behavioral responses of male CB57BL/6 mice during exposure to 20% CO2 or 7% O2 and its consequence on plasma levels of corticosterone. We also evaluated whether clinically-effective panicolytic drugs affect the behavioral responses expressed during CO2 exposure. The results showed that whereas hypoxia caused a marked reduction in locomotion, inhalation of CO2-enriched air evoked an active escape response, characterized by bouts of upward leaps directed to the border of the experimental cage, interpreted as escape attempts. Corticosterone levels were increased 30 min after either of the respiratory challenges used, but it was higher in the hypoxia group. Chronic (21 days), but not acute, treatment with fluoxetine or imipramine (5, 10 or 15 mg/kg) or a single injection of alprazolam (0.025, 0.05 or 0.1 mg/kg), but not of the anxiolytic diazepam (0.025, 0.05 or 0.1 and 1 mg/kg) reduced the number of escape attempts, indicating a panicolytic-like effect. Altogether, the results suggest that whereas hypoxia increased anxiety, exposure to 20% CO2 evoked a panic-like state. The latter condition/test protocol seems to be a simple and validated model for studying in mice pathophysiological mechanisms and the screening of novel drugs for panic disorder. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:15:56Z 2018-12-11T17:15:56Z 2018-02-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pnpbp.2017.10.018 Progress in Neuro-Psychopharmacology and Biological Psychiatry, v. 81, p. 178-186. 1878-4216 0278-5846 http://hdl.handle.net/11449/175462 10.1016/j.pnpbp.2017.10.018 2-s2.0-85033409473 2-s2.0-85033409473.pdf |
url |
http://dx.doi.org/10.1016/j.pnpbp.2017.10.018 http://hdl.handle.net/11449/175462 |
identifier_str_mv |
Progress in Neuro-Psychopharmacology and Biological Psychiatry, v. 81, p. 178-186. 1878-4216 0278-5846 10.1016/j.pnpbp.2017.10.018 2-s2.0-85033409473 2-s2.0-85033409473.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Progress in Neuro-Psychopharmacology and Biological Psychiatry 1,714 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
178-186 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965649736302592 |