Lethal photosensitization in microbiological treatment of ligature-induced peri-implantitis: a preliminary study in dogs

• Autor(a) principal: Shibli, Jamil Awad
• Data de Publicação: 2003
• Outros Autores: Martins, Marilia Compagnoni, Theodoro, Letícia Helena [UNESP], Lotufo, Roberto Fraga Moreira, Garcia, Valdir Gouveia [UNESP], Marcantonio, Elcio Júnior
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.2334/josnusd.45.17; http://hdl.handle.net/11449/67218
• topic: photosensitizing agent; tolonium chloride; anaerobic bacterium; animal; bacterial count; dog; drug effect; Fusobacterium; laser; ligation; microbiology; nonparametric test; periodontitis; pilot study; Porphyromonas gingivalis; Prevotella intermedia; Streptococcus; tooth implantation; Animals; Bacteria, Anaerobic; Colony Count, Microbial; Dental Implants; Dogs; Lasers; Ligation; Periodontitis; Photosensitizing Agents; Pilot Projects; Statistics, Nonparametric; Tolonium Chloride

This pilot study evaluated, by culture testing, the effectiveness of lethal photosensitization for the microbiological treatment of peri-implantitis in dogs. Experimental peri-implantitis was induced by ligature placement for 2 months. Following ligature removal, plaque control was instituted by scrubbing with 0.12% chlorhexidine daily for 12 months. Subsequently, mucoperiosteal flaps were elevated for scaling the implant surface. Microbial samples were obtained with paper points before and after treatment of implant surfaces by means of 100 microg/ml toluidine blue O (TBO,) and were exposed, for 80 s, to light with a wavelength of 685 nm from a 50 mW GaAlAs diode laser. The mean initial and final bacterial counts were 7.22 +/- 0.20 and 6.84 +/- 0.44 CFU/ml, respectively for TVC (P < 0.0001); 6.19 +/- 0.45 and 3.14 +/- 3.29 CFU/ml for P. intermedia/nigrescens (P = 0.001); 5.98 +/- 0.38 and 1.69 +/- 2.90 CFU/ml for Fusobacterium spp. (P = 0.001); and 6.07 +/- 0.22 to 1.69 +/- 2.94 CFU/ml for beta-hemolytic Streptococcus (P = 0.0039). It may be concluded that lethal photosensitization resulted in a reduction of the bacterial count. Complete elimination of bacteria was achieved in some samples.