Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0184073 http://hdl.handle.net/11449/165739 |
Resumo: | To avoid certain problems encountered with more-traditional and invasive methods in behavioral-ecology studies of mammalian predators, such as felids, molecular approaches have been employed to identify feces found in the field. However, this method requires a complete molecular biology laboratory, and usually also requires very fresh fecal samples to avoid DNA degradation. Both conditions are normally absent in the field. To address these difficulties, identification based on morphological characters (length, color, banding, scales and medullar patterns) of hairs found in feces could be employed as an alternative. In this study we constructed a morphological identification key for guard hairs of eight Neotropical felids (jaguar, oncilla, Geoffroy's cat, margay, ocelot, Pampas cat, puma and jaguarundi) and compared its efficiency to that of a molecular identification method, using the ATP6 region as a marker. For this molecular approach, we simulated some field conditions by postponing sample-conservation procedures. A blind test of the identification key obtained a nearly 70% overall success rate, which we considered equivalent to or better than the results of some molecular methods (probably due to DNA degradation) found in other studies. The jaguar, puma and jaguarundi could be unequivocally discriminated from any other Neotropical felid. On a scale ranging from inadequate to excellent, the key proved poor only for the margay, with only 30% of its hairs successfully identified using this key; and have intermediate success rates for the remaining species, the oncilla, Geoffroy's cat, ocelot and Pampas cat, were intermediate. Complementary information about the known distributions of felid populations may be necessary to substantially improve the results obtained with the key. Our own molecular results were even better, since all blind-tested samples were correctly identified. Part of these identifications were made from samples kept in suboptimal conditions, with some samples remaining outdoors for up to seven days, simulating conditions in the field. It appears that both methods can be used, depending on the available laboratory facilities and on the expected results. |
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Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felidsTo avoid certain problems encountered with more-traditional and invasive methods in behavioral-ecology studies of mammalian predators, such as felids, molecular approaches have been employed to identify feces found in the field. However, this method requires a complete molecular biology laboratory, and usually also requires very fresh fecal samples to avoid DNA degradation. Both conditions are normally absent in the field. To address these difficulties, identification based on morphological characters (length, color, banding, scales and medullar patterns) of hairs found in feces could be employed as an alternative. In this study we constructed a morphological identification key for guard hairs of eight Neotropical felids (jaguar, oncilla, Geoffroy's cat, margay, ocelot, Pampas cat, puma and jaguarundi) and compared its efficiency to that of a molecular identification method, using the ATP6 region as a marker. For this molecular approach, we simulated some field conditions by postponing sample-conservation procedures. A blind test of the identification key obtained a nearly 70% overall success rate, which we considered equivalent to or better than the results of some molecular methods (probably due to DNA degradation) found in other studies. The jaguar, puma and jaguarundi could be unequivocally discriminated from any other Neotropical felid. On a scale ranging from inadequate to excellent, the key proved poor only for the margay, with only 30% of its hairs successfully identified using this key; and have intermediate success rates for the remaining species, the oncilla, Geoffroy's cat, ocelot and Pampas cat, were intermediate. Complementary information about the known distributions of felid populations may be necessary to substantially improve the results obtained with the key. Our own molecular results were even better, since all blind-tested samples were correctly identified. Part of these identifications were made from samples kept in suboptimal conditions, with some samples remaining outdoors for up to seven days, simulating conditions in the field. It appears that both methods can be used, depending on the available laboratory facilities and on the expected results.Univ Estadual Paulista, Fac Ciencias & Letras Assis, LEvEtho Lab Evolut & Ethol, Assis, SP, BrazilUniv Fed Sao Carlos, Dept Genet & Evolucao, LabBMC Lab Biodiversidade Mol & Conservacao, Sao Carlos, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Letras Assis, LEA, Assis, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Letras Assis, LEvEtho Lab Evolut & Ethol, Assis, SP, BrazilUniv Estadual Paulista, Fac Ciencias & Letras Assis, LEA, Assis, SP, Brazil: Fpaesp 04/08187-3: Fapesp 2013/24453-4: CNPq 308385/2014-4Public Library ScienceUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Alberts, Carlos C. [UNESP]Saranholi, Bruno H.Frei, Fernando [UNESP]Galetti, Pedro M.2018-11-28T19:09:00Z2018-11-28T19:09:00Z2017-09-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article24application/pdfhttp://dx.doi.org/10.1371/journal.pone.0184073Plos One. San Francisco: Public Library Science, v. 12, n. 9, 24 p., 2017.1932-6203http://hdl.handle.net/11449/16573910.1371/journal.pone.0184073WOS:000409467200033WOS000409467200033.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One1,164info:eu-repo/semantics/openAccess2023-10-22T06:12:48Zoai:repositorio.unesp.br:11449/165739Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-22T06:12:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids |
title |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids |
spellingShingle |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids Alberts, Carlos C. [UNESP] |
title_short |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids |
title_full |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids |
title_fullStr |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids |
title_full_unstemmed |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids |
title_sort |
Comparing hair-morphology and molecular methods to identify fecal samples from Neotropical felids |
author |
Alberts, Carlos C. [UNESP] |
author_facet |
Alberts, Carlos C. [UNESP] Saranholi, Bruno H. Frei, Fernando [UNESP] Galetti, Pedro M. |
author_role |
author |
author2 |
Saranholi, Bruno H. Frei, Fernando [UNESP] Galetti, Pedro M. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Alberts, Carlos C. [UNESP] Saranholi, Bruno H. Frei, Fernando [UNESP] Galetti, Pedro M. |
description |
To avoid certain problems encountered with more-traditional and invasive methods in behavioral-ecology studies of mammalian predators, such as felids, molecular approaches have been employed to identify feces found in the field. However, this method requires a complete molecular biology laboratory, and usually also requires very fresh fecal samples to avoid DNA degradation. Both conditions are normally absent in the field. To address these difficulties, identification based on morphological characters (length, color, banding, scales and medullar patterns) of hairs found in feces could be employed as an alternative. In this study we constructed a morphological identification key for guard hairs of eight Neotropical felids (jaguar, oncilla, Geoffroy's cat, margay, ocelot, Pampas cat, puma and jaguarundi) and compared its efficiency to that of a molecular identification method, using the ATP6 region as a marker. For this molecular approach, we simulated some field conditions by postponing sample-conservation procedures. A blind test of the identification key obtained a nearly 70% overall success rate, which we considered equivalent to or better than the results of some molecular methods (probably due to DNA degradation) found in other studies. The jaguar, puma and jaguarundi could be unequivocally discriminated from any other Neotropical felid. On a scale ranging from inadequate to excellent, the key proved poor only for the margay, with only 30% of its hairs successfully identified using this key; and have intermediate success rates for the remaining species, the oncilla, Geoffroy's cat, ocelot and Pampas cat, were intermediate. Complementary information about the known distributions of felid populations may be necessary to substantially improve the results obtained with the key. Our own molecular results were even better, since all blind-tested samples were correctly identified. Part of these identifications were made from samples kept in suboptimal conditions, with some samples remaining outdoors for up to seven days, simulating conditions in the field. It appears that both methods can be used, depending on the available laboratory facilities and on the expected results. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-07 2018-11-28T19:09:00Z 2018-11-28T19:09:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0184073 Plos One. San Francisco: Public Library Science, v. 12, n. 9, 24 p., 2017. 1932-6203 http://hdl.handle.net/11449/165739 10.1371/journal.pone.0184073 WOS:000409467200033 WOS000409467200033.pdf |
url |
http://dx.doi.org/10.1371/journal.pone.0184073 http://hdl.handle.net/11449/165739 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 12, n. 9, 24 p., 2017. 1932-6203 10.1371/journal.pone.0184073 WOS:000409467200033 WOS000409467200033.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
24 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799964662039576576 |