Effects of uterine cervix constriction on Wistar rats
Main Author: | |
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Publication Date: | 2010 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://dx.doi.org/10.1590/S0102-86502010000600002 http://hdl.handle.net/11449/231968 |
Summary: | Purpose: To verify if uterine cerclage can induce craniosynostosis or any cranial deformity in new born Wistar rats. Methods: One pregnant female Wistar rat underwent laparotomy on day 18 of gestation and the uterus cervix was closed with a 3-0 nylon suture to avoid delivery, that occurs normally on the 21 day. The suture was released after 48 hours beyond the normal gestation period. The female rat delivered 11 pups. Six surviving rats from the delivery (group A - constrained group). Two rats were born from another mother and in the same age were used as control group (group B - 2 nonconstrained controls) were allowed to grow. They were sacrificed 1.2 years after their birth all the eight animals. Linear measurement, routine histology and computed tomography of the skull were performed at the time of their death to evaluate the cranial asymmetries by mesurements of the anatomical landmarks of the craniofacial skeleton of the rats on the two groups and compared then. Results: We did not observe statistically significant differences in any of the compared measurements (p>0.05) obtained through the morphologic and radiologic methods. Histologic examinations did not reveal any sign of premature fusion or suture imbrications. Critical decrease in longitudinal body size was noticed as the limbs too in all the animals of group A. Conclusion: Constriction of uterine cervix leads to fetus suffering, even death for a few animals, associated to small body size, but not to craniosynostosis. |
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Effects of uterine cervix constriction on Wistar ratsCerclage, cervicalCraniosynostosesRatsSurgery, plasticPurpose: To verify if uterine cerclage can induce craniosynostosis or any cranial deformity in new born Wistar rats. Methods: One pregnant female Wistar rat underwent laparotomy on day 18 of gestation and the uterus cervix was closed with a 3-0 nylon suture to avoid delivery, that occurs normally on the 21 day. The suture was released after 48 hours beyond the normal gestation period. The female rat delivered 11 pups. Six surviving rats from the delivery (group A - constrained group). Two rats were born from another mother and in the same age were used as control group (group B - 2 nonconstrained controls) were allowed to grow. They were sacrificed 1.2 years after their birth all the eight animals. Linear measurement, routine histology and computed tomography of the skull were performed at the time of their death to evaluate the cranial asymmetries by mesurements of the anatomical landmarks of the craniofacial skeleton of the rats on the two groups and compared then. Results: We did not observe statistically significant differences in any of the compared measurements (p>0.05) obtained through the morphologic and radiologic methods. Histologic examinations did not reveal any sign of premature fusion or suture imbrications. Critical decrease in longitudinal body size was noticed as the limbs too in all the animals of group A. Conclusion: Constriction of uterine cervix leads to fetus suffering, even death for a few animals, associated to small body size, but not to craniosynostosis.Institute of Craniofacial and Plastic Surgery SOBRAPAR, Campinas-SPVeterinary Medicine State University of Sao Paulo (UNESP), Botucatu-SPDepartment of Genetics and Evolution Biology Institute of Biosciences USP, Sao PauloPlastic Surgery Division Department of Surgery USP, Sao PauloVeterinary Medicine State University of Sao Paulo (UNESP), Botucatu-SPSOBRAPARUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Raposo-Amaral, Cassio EduardoAlmeida, Ana BeatrizRaposo-Amaral, Cesar AugustoVulcano, Luiz Carlos [UNESP]Passos-Bueno, Maria RitaAlonso, Nivaldo2022-04-29T08:48:22Z2022-04-29T08:48:22Z2010-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article469-474http://dx.doi.org/10.1590/S0102-86502010000600002Acta Cirurgica Brasileira, v. 25, n. 6, p. 469-474, 2010.0102-86501678-2674http://hdl.handle.net/11449/23196810.1590/S0102-865020100006000022-s2.0-78650505932Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccess2022-04-29T08:48:23Zoai:repositorio.unesp.br:11449/231968Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:48:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of uterine cervix constriction on Wistar rats |
title |
Effects of uterine cervix constriction on Wistar rats |
spellingShingle |
Effects of uterine cervix constriction on Wistar rats Raposo-Amaral, Cassio Eduardo Cerclage, cervical Craniosynostoses Rats Surgery, plastic |
title_short |
Effects of uterine cervix constriction on Wistar rats |
title_full |
Effects of uterine cervix constriction on Wistar rats |
title_fullStr |
Effects of uterine cervix constriction on Wistar rats |
title_full_unstemmed |
Effects of uterine cervix constriction on Wistar rats |
title_sort |
Effects of uterine cervix constriction on Wistar rats |
author |
Raposo-Amaral, Cassio Eduardo |
author_facet |
Raposo-Amaral, Cassio Eduardo Almeida, Ana Beatriz Raposo-Amaral, Cesar Augusto Vulcano, Luiz Carlos [UNESP] Passos-Bueno, Maria Rita Alonso, Nivaldo |
author_role |
author |
author2 |
Almeida, Ana Beatriz Raposo-Amaral, Cesar Augusto Vulcano, Luiz Carlos [UNESP] Passos-Bueno, Maria Rita Alonso, Nivaldo |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
SOBRAPAR Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Raposo-Amaral, Cassio Eduardo Almeida, Ana Beatriz Raposo-Amaral, Cesar Augusto Vulcano, Luiz Carlos [UNESP] Passos-Bueno, Maria Rita Alonso, Nivaldo |
dc.subject.por.fl_str_mv |
Cerclage, cervical Craniosynostoses Rats Surgery, plastic |
topic |
Cerclage, cervical Craniosynostoses Rats Surgery, plastic |
description |
Purpose: To verify if uterine cerclage can induce craniosynostosis or any cranial deformity in new born Wistar rats. Methods: One pregnant female Wistar rat underwent laparotomy on day 18 of gestation and the uterus cervix was closed with a 3-0 nylon suture to avoid delivery, that occurs normally on the 21 day. The suture was released after 48 hours beyond the normal gestation period. The female rat delivered 11 pups. Six surviving rats from the delivery (group A - constrained group). Two rats were born from another mother and in the same age were used as control group (group B - 2 nonconstrained controls) were allowed to grow. They were sacrificed 1.2 years after their birth all the eight animals. Linear measurement, routine histology and computed tomography of the skull were performed at the time of their death to evaluate the cranial asymmetries by mesurements of the anatomical landmarks of the craniofacial skeleton of the rats on the two groups and compared then. Results: We did not observe statistically significant differences in any of the compared measurements (p>0.05) obtained through the morphologic and radiologic methods. Histologic examinations did not reveal any sign of premature fusion or suture imbrications. Critical decrease in longitudinal body size was noticed as the limbs too in all the animals of group A. Conclusion: Constriction of uterine cervix leads to fetus suffering, even death for a few animals, associated to small body size, but not to craniosynostosis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-01 2022-04-29T08:48:22Z 2022-04-29T08:48:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502010000600002 Acta Cirurgica Brasileira, v. 25, n. 6, p. 469-474, 2010. 0102-8650 1678-2674 http://hdl.handle.net/11449/231968 10.1590/S0102-86502010000600002 2-s2.0-78650505932 |
url |
http://dx.doi.org/10.1590/S0102-86502010000600002 http://hdl.handle.net/11449/231968 |
identifier_str_mv |
Acta Cirurgica Brasileira, v. 25, n. 6, p. 469-474, 2010. 0102-8650 1678-2674 10.1590/S0102-86502010000600002 2-s2.0-78650505932 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirurgica Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
469-474 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965303121117184 |