Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1002/tox.23598 http://hdl.handle.net/11449/241904 |
Resumo: | The possibility of chemical contamination is an important issue to consider when designing a cell therapy strategy. Both bisphenol A (BPA) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are among the most environmentally relevant endocrine disrupting chemicals (EDCs, compounds with a high affinity for adipose tissue) recently studied. Adipose-derived stem cells (ASCs) are mesenchymal stromal cells (MSCs) obtained from adipose tissue widely used in regenerative medicine to prevent and treat diseases in several tissues and organs. Although the experimental use of tissue-engineered constructs requires careful analysis for approval and implantation, there has been a recent increase in the number of approved clinical trials for this promising strategy. This study aimed to evaluate cell viability, apoptosis, DNA damage, and the adipogenic or osteogenic differentiation potential of rat adipose-derived stem cells (rASCs) exposed to previously established non-cytotoxic doses of BPA and TCDD in vitro. Results demonstrated that 10 μM of BPA and 10 nM of TCDD were able to significantly reduce cell viability, while all exposure levels resulted in DNA damage, although did not increase the apoptosis rate. According to the analysis of adipogenic differentiation, 1 μM of BPA induced the significant formation of oil droplets, suggesting an increased adipocyte differentiation, while both 10 μM of BPA and 10 nM of TCDD decreased adipocyte differentiation. Osteogenic differentiation did not differ among the treatments. As such, BPA and TCDD in the concentrations tested can modify important processes in rASCs such as cell viability, adipogenic differentiation, and DNA damage. Together, these findings prove that EDCs play an important role as contaminants, putatively interfering in cell differentiation and thus impairing the therapeutic use of ASCs. |
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Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cellsBPADNA damagerASCsregenerative medicineTCDDThe possibility of chemical contamination is an important issue to consider when designing a cell therapy strategy. Both bisphenol A (BPA) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are among the most environmentally relevant endocrine disrupting chemicals (EDCs, compounds with a high affinity for adipose tissue) recently studied. Adipose-derived stem cells (ASCs) are mesenchymal stromal cells (MSCs) obtained from adipose tissue widely used in regenerative medicine to prevent and treat diseases in several tissues and organs. Although the experimental use of tissue-engineered constructs requires careful analysis for approval and implantation, there has been a recent increase in the number of approved clinical trials for this promising strategy. This study aimed to evaluate cell viability, apoptosis, DNA damage, and the adipogenic or osteogenic differentiation potential of rat adipose-derived stem cells (rASCs) exposed to previously established non-cytotoxic doses of BPA and TCDD in vitro. Results demonstrated that 10 μM of BPA and 10 nM of TCDD were able to significantly reduce cell viability, while all exposure levels resulted in DNA damage, although did not increase the apoptosis rate. According to the analysis of adipogenic differentiation, 1 μM of BPA induced the significant formation of oil droplets, suggesting an increased adipocyte differentiation, while both 10 μM of BPA and 10 nM of TCDD decreased adipocyte differentiation. Osteogenic differentiation did not differ among the treatments. As such, BPA and TCDD in the concentrations tested can modify important processes in rASCs such as cell viability, adipogenic differentiation, and DNA damage. Together, these findings prove that EDCs play an important role as contaminants, putatively interfering in cell differentiation and thus impairing the therapeutic use of ASCs.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Sao PauloBotucatu Medical School Blood Transfusion Center Cell Engineering Lab São Paulo State University (UNESP), Sao PauloBotucatu Medical School Blood Transfusion Center Flow Cytometry Laboratory São Paulo State University (UNESP), Sao PauloDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), Sao PauloDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), Sao PauloBotucatu Medical School Blood Transfusion Center Cell Engineering Lab São Paulo State University (UNESP), Sao PauloBotucatu Medical School Blood Transfusion Center Flow Cytometry Laboratory São Paulo State University (UNESP), Sao PauloDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), Sao PauloUniversidade Estadual Paulista (UNESP)Nunes, Helga Caputo [UNESP]Tavares, Samara Costa [UNESP]Garcia, Heloísa Vicente [UNESP]Cucielo, Maira Smaniotto [UNESP]dos Santos, Sérgio Alexandre Alcântara [UNESP]Aal, Mirian Carolini Esgoti [UNESP]de Golim, Marjorie Assis [UNESP]Justulin, Luís Antônio [UNESP]Ribeiro, Amanda Oliveira [UNESP]Deffune, Elenice [UNESP]Scarano, Wellerson Rodrigo [UNESP]Delella, Flávia Karina [UNESP]2023-03-02T03:34:20Z2023-03-02T03:34:20Z2022-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2314-2323http://dx.doi.org/10.1002/tox.23598Environmental Toxicology, v. 37, n. 9, p. 2314-2323, 2022.1522-72781520-4081http://hdl.handle.net/11449/24190410.1002/tox.235982-s2.0-85131198623Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Toxicologyinfo:eu-repo/semantics/openAccess2023-03-02T03:34:20Zoai:repositorio.unesp.br:11449/241904Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T03:34:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells |
title |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells |
spellingShingle |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells Nunes, Helga Caputo [UNESP] BPA DNA damage rASCs regenerative medicine TCDD |
title_short |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells |
title_full |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells |
title_fullStr |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells |
title_full_unstemmed |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells |
title_sort |
Bisphenol A and 2,3,7,8-tetrachlorodibenzo-p-dioxin at non-cytotoxic doses alter the differentiation potential and cell function of rat adipose-stem cells |
author |
Nunes, Helga Caputo [UNESP] |
author_facet |
Nunes, Helga Caputo [UNESP] Tavares, Samara Costa [UNESP] Garcia, Heloísa Vicente [UNESP] Cucielo, Maira Smaniotto [UNESP] dos Santos, Sérgio Alexandre Alcântara [UNESP] Aal, Mirian Carolini Esgoti [UNESP] de Golim, Marjorie Assis [UNESP] Justulin, Luís Antônio [UNESP] Ribeiro, Amanda Oliveira [UNESP] Deffune, Elenice [UNESP] Scarano, Wellerson Rodrigo [UNESP] Delella, Flávia Karina [UNESP] |
author_role |
author |
author2 |
Tavares, Samara Costa [UNESP] Garcia, Heloísa Vicente [UNESP] Cucielo, Maira Smaniotto [UNESP] dos Santos, Sérgio Alexandre Alcântara [UNESP] Aal, Mirian Carolini Esgoti [UNESP] de Golim, Marjorie Assis [UNESP] Justulin, Luís Antônio [UNESP] Ribeiro, Amanda Oliveira [UNESP] Deffune, Elenice [UNESP] Scarano, Wellerson Rodrigo [UNESP] Delella, Flávia Karina [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Nunes, Helga Caputo [UNESP] Tavares, Samara Costa [UNESP] Garcia, Heloísa Vicente [UNESP] Cucielo, Maira Smaniotto [UNESP] dos Santos, Sérgio Alexandre Alcântara [UNESP] Aal, Mirian Carolini Esgoti [UNESP] de Golim, Marjorie Assis [UNESP] Justulin, Luís Antônio [UNESP] Ribeiro, Amanda Oliveira [UNESP] Deffune, Elenice [UNESP] Scarano, Wellerson Rodrigo [UNESP] Delella, Flávia Karina [UNESP] |
dc.subject.por.fl_str_mv |
BPA DNA damage rASCs regenerative medicine TCDD |
topic |
BPA DNA damage rASCs regenerative medicine TCDD |
description |
The possibility of chemical contamination is an important issue to consider when designing a cell therapy strategy. Both bisphenol A (BPA) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are among the most environmentally relevant endocrine disrupting chemicals (EDCs, compounds with a high affinity for adipose tissue) recently studied. Adipose-derived stem cells (ASCs) are mesenchymal stromal cells (MSCs) obtained from adipose tissue widely used in regenerative medicine to prevent and treat diseases in several tissues and organs. Although the experimental use of tissue-engineered constructs requires careful analysis for approval and implantation, there has been a recent increase in the number of approved clinical trials for this promising strategy. This study aimed to evaluate cell viability, apoptosis, DNA damage, and the adipogenic or osteogenic differentiation potential of rat adipose-derived stem cells (rASCs) exposed to previously established non-cytotoxic doses of BPA and TCDD in vitro. Results demonstrated that 10 μM of BPA and 10 nM of TCDD were able to significantly reduce cell viability, while all exposure levels resulted in DNA damage, although did not increase the apoptosis rate. According to the analysis of adipogenic differentiation, 1 μM of BPA induced the significant formation of oil droplets, suggesting an increased adipocyte differentiation, while both 10 μM of BPA and 10 nM of TCDD decreased adipocyte differentiation. Osteogenic differentiation did not differ among the treatments. As such, BPA and TCDD in the concentrations tested can modify important processes in rASCs such as cell viability, adipogenic differentiation, and DNA damage. Together, these findings prove that EDCs play an important role as contaminants, putatively interfering in cell differentiation and thus impairing the therapeutic use of ASCs. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-01 2023-03-02T03:34:20Z 2023-03-02T03:34:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1002/tox.23598 Environmental Toxicology, v. 37, n. 9, p. 2314-2323, 2022. 1522-7278 1520-4081 http://hdl.handle.net/11449/241904 10.1002/tox.23598 2-s2.0-85131198623 |
url |
http://dx.doi.org/10.1002/tox.23598 http://hdl.handle.net/11449/241904 |
identifier_str_mv |
Environmental Toxicology, v. 37, n. 9, p. 2314-2323, 2022. 1522-7278 1520-4081 10.1002/tox.23598 2-s2.0-85131198623 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Toxicology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2314-2323 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790092746555392 |