Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng spa |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4067/S0717-95022012000300061 http://hdl.handle.net/11449/227135 |
Resumo: | The morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into two fragments, which were used for histological (H.E.) and histoenzymological (NADH-TR and myofibrillar ATPase). After 4 weeks, denervated muscle fibers showed important light microscopic alterations: atrophy with angular profiles in cross-sections, cytoplasm containing vacuoles, enlarged interstitial space with increased connective tissue, cellular infiltration, and muscle fibers without defined contours. The most marked alterations being observed for type IIb and IIa fibers. Eight and 12 weeks after denervation, the NADH-TR reaction showed that it was impossible to characterize the muscle fibers based on their metabolic profile. |
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Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en RatasMorphologic alterations resulting from denervation of the diaphragm in ratsDenervationDiaphragmMuscle fiberRatThe morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into two fragments, which were used for histological (H.E.) and histoenzymological (NADH-TR and myofibrillar ATPase). After 4 weeks, denervated muscle fibers showed important light microscopic alterations: atrophy with angular profiles in cross-sections, cytoplasm containing vacuoles, enlarged interstitial space with increased connective tissue, cellular infiltration, and muscle fibers without defined contours. The most marked alterations being observed for type IIb and IIa fibers. Eight and 12 weeks after denervation, the NADH-TR reaction showed that it was impossible to characterize the muscle fibers based on their metabolic profile.Medical and Pharmaceutic Sciences Center UNIOESTE, Cascavel, ParanáDepartment of Anatomy IB UNESP, Botucatu, SPDepartment of Morphology Sciences UEM, Maringá, ParanáDepartment of Physiotherapy UFJF, Juiz de Fora, Minas GeraisDepartment of Anatomy IB UNESP, Botucatu, SPUNIOESTEUniversidade Estadual Paulista (UNESP)Universidade Estadual de Maringá (UEM)UFJFTorrejais, Marcia MirandaSoares, J. C. [UNESP]Matheus, S. M.M. [UNESP]Francia-Farje, L. A.D. [UNESP]Mello, J. M.Vicente, E. J.D.2022-04-29T06:48:24Z2022-04-29T06:48:24Z2012-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1150-1157http://dx.doi.org/10.4067/S0717-95022012000300061International Journal of Morphology, v. 30, n. 3, p. 1150-1157, 2012.0717-93670717-9502http://hdl.handle.net/11449/22713510.4067/S0717-950220120003000612-s2.0-84874617169Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengspaInternational Journal of Morphologyinfo:eu-repo/semantics/openAccess2022-04-29T06:48:24Zoai:repositorio.unesp.br:11449/227135Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T06:48:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas Morphologic alterations resulting from denervation of the diaphragm in rats |
title |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas |
spellingShingle |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas Torrejais, Marcia Miranda Denervation Diaphragm Muscle fiber Rat |
title_short |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas |
title_full |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas |
title_fullStr |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas |
title_full_unstemmed |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas |
title_sort |
Alteraciones Morfológicas Resultantes de la Denervación del Diafragma en Ratas |
author |
Torrejais, Marcia Miranda |
author_facet |
Torrejais, Marcia Miranda Soares, J. C. [UNESP] Matheus, S. M.M. [UNESP] Francia-Farje, L. A.D. [UNESP] Mello, J. M. Vicente, E. J.D. |
author_role |
author |
author2 |
Soares, J. C. [UNESP] Matheus, S. M.M. [UNESP] Francia-Farje, L. A.D. [UNESP] Mello, J. M. Vicente, E. J.D. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
UNIOESTE Universidade Estadual Paulista (UNESP) Universidade Estadual de Maringá (UEM) UFJF |
dc.contributor.author.fl_str_mv |
Torrejais, Marcia Miranda Soares, J. C. [UNESP] Matheus, S. M.M. [UNESP] Francia-Farje, L. A.D. [UNESP] Mello, J. M. Vicente, E. J.D. |
dc.subject.por.fl_str_mv |
Denervation Diaphragm Muscle fiber Rat |
topic |
Denervation Diaphragm Muscle fiber Rat |
description |
The morphological alterations that occur in the muscle fibers of denervated rat diaphragms were studied. Fifteen adult male albino rats (Rattus norvegicus) with a mean weight of 200 g and about 60 days of age were used. Chronically denervated diaphragms were obtained and the animals were sacrificed after 4, 8 and 12 weeks of denervation. The left antimere of the diaphragm was denervated by sectioning of the phrenic nerve and the right antimere served as control. Each antimere was divided into two fragments, which were used for histological (H.E.) and histoenzymological (NADH-TR and myofibrillar ATPase). After 4 weeks, denervated muscle fibers showed important light microscopic alterations: atrophy with angular profiles in cross-sections, cytoplasm containing vacuoles, enlarged interstitial space with increased connective tissue, cellular infiltration, and muscle fibers without defined contours. The most marked alterations being observed for type IIb and IIa fibers. Eight and 12 weeks after denervation, the NADH-TR reaction showed that it was impossible to characterize the muscle fibers based on their metabolic profile. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 2022-04-29T06:48:24Z 2022-04-29T06:48:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4067/S0717-95022012000300061 International Journal of Morphology, v. 30, n. 3, p. 1150-1157, 2012. 0717-9367 0717-9502 http://hdl.handle.net/11449/227135 10.4067/S0717-95022012000300061 2-s2.0-84874617169 |
url |
http://dx.doi.org/10.4067/S0717-95022012000300061 http://hdl.handle.net/11449/227135 |
identifier_str_mv |
International Journal of Morphology, v. 30, n. 3, p. 1150-1157, 2012. 0717-9367 0717-9502 10.4067/S0717-95022012000300061 2-s2.0-84874617169 |
dc.language.iso.fl_str_mv |
eng spa |
language |
eng spa |
dc.relation.none.fl_str_mv |
International Journal of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1150-1157 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789446048841728 |