Análise histopatológica da biocompatibilidade da pasta tri-antibiótica à base de ciprofloxacina, minociclina e metronidazol em tecido subcutâneo de ratos

• Autor(a) principal: Oliveira, Claudiel Batista de [UNESP]
• Data de Publicação: 2012
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149451; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000855584.pdf
• topic: Biocompatibilidade; Ciprofloxacino; Minociclina; Metronidazol; Ciprofloxacin

The aim of this study was to evaluate the tissue response to implanted polyethylene tubes filled with tri-antibiotic paste or calcium hydroxide paste in subcutaneous tissue of rats. Thirty rats was individually receive three polyethylene tubes, two filled with tri-antibiotic paste or calcium hydroxide paste and one more empty tube as control. The observation periods was 7, 15, 30, 60 e 90 days. After each period of time, six animals was killed and the tubes and surrounding tissue removed, fixed and prepared to be analyzed in light microscope with glycol methacrylate embedding, 3 m serial cutting and HE staining. Scores was defined as follows: 0, none or few inflammatory cells, no reaction; 1, <25 cells, mild reaction; 2, 25 to 125 cells, moderate reaction; 3, >125 cells, severe reaction. Fibrous capsule was categorized as thin when thickness was < 150 μm and thick at > 150 μm. Necrosis and formation of calcification were recorded. The data was statistically analyzed using Kruskal Wallis test. The three groups showed reduced inflammatory reaction after 30, 60 and 90 days and moderate inflammatory reaction at 7 and 15 days. There was no statistically significant difference (p> 0.05) between the periods of 7 and 15 and between the periods 30, 60 and 90 days