Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1017/S0967199419000741 http://hdl.handle.net/11449/208868 |
Resumo: | The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (<= 3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P> 0.05) was observed between small/medium and large follicles: 49.2 +/- 22.8 and 56.7 +/- 27.4 mu g/mu l, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P> 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer. |
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Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814)DeerElectrophoresisMSSDS-PAGEThe aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (<= 3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P> 0.05) was observed between small/medium and large follicles: 49.2 +/- 22.8 and 56.7 +/- 27.4 mu g/mu l, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P> 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)(FUNCAP)Univ Estadual Ceara, Fac Vet, Av Dr Silas Munguba 1700, BR-60714903 Fortaleza, Ceara, BrazilUniv Fed Ceara, Dept Anim Sci, Fortaleza, Ceara, BrazilUniv Estadual Ceara, Hlth Sci Ctr, Fortaleza, Ceara, BrazilUniv Fed Ceara, Lab Mass Spectrometry, Fortaleza, Ceara, BrazilState Univ Sao Paulo, Dept Anim Sci, Jaboticabal, BrazilUniv Ctr Fametro, Mol Genet Res Unit, Fortaleza, Ceara, BrazilState Univ Sao Paulo, Dept Anim Sci, Jaboticabal, BrazilCNPq: 461330/2014-8(FUNCAP): CI3-0093-0005580100/14Cambridge Univ PressUniv Estadual CearaUniv Fed CearaUniversidade Estadual Paulista (Unesp)Univ Ctr FametroSouza, Thais T. S.Bezerra, Maria J. B.van Tilburg, Mauricio F.Nagano, Celso S.Rola, Luciana D. [UNESP]Duarte, Jose M. B. [UNESP]Melo, Luciana M.Moura, Arlindo A.Freitas, Vicente J. F.2021-06-25T11:23:13Z2021-06-25T11:23:13Z2020-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article170-173http://dx.doi.org/10.1017/S0967199419000741Zygote. New York: Cambridge Univ Press, v. 28, n. 2, p. 170-173, 2020.0967-1994http://hdl.handle.net/11449/20886810.1017/S0967199419000741WOS:000578224800012Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygoteinfo:eu-repo/semantics/openAccess2021-10-23T19:23:15Zoai:repositorio.unesp.br:11449/208868Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:15Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) |
| title |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) |
| spellingShingle |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) Souza, Thais T. S. Deer Electrophoresis MS SDS-PAGE |
| title_short |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) |
| title_full |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) |
| title_fullStr |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) |
| title_full_unstemmed |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) |
| title_sort |
Protein profile of the ovarian follicular fluid of brown brocket deer (Mazama gouazoubira; Fisher, 1814) |
| author |
Souza, Thais T. S. |
| author_facet |
Souza, Thais T. S. Bezerra, Maria J. B. van Tilburg, Mauricio F. Nagano, Celso S. Rola, Luciana D. [UNESP] Duarte, Jose M. B. [UNESP] Melo, Luciana M. Moura, Arlindo A. Freitas, Vicente J. F. |
| author_role |
author |
| author2 |
Bezerra, Maria J. B. van Tilburg, Mauricio F. Nagano, Celso S. Rola, Luciana D. [UNESP] Duarte, Jose M. B. [UNESP] Melo, Luciana M. Moura, Arlindo A. Freitas, Vicente J. F. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Univ Estadual Ceara Univ Fed Ceara Universidade Estadual Paulista (Unesp) Univ Ctr Fametro |
| dc.contributor.author.fl_str_mv |
Souza, Thais T. S. Bezerra, Maria J. B. van Tilburg, Mauricio F. Nagano, Celso S. Rola, Luciana D. [UNESP] Duarte, Jose M. B. [UNESP] Melo, Luciana M. Moura, Arlindo A. Freitas, Vicente J. F. |
| dc.subject.por.fl_str_mv |
Deer Electrophoresis MS SDS-PAGE |
| topic |
Deer Electrophoresis MS SDS-PAGE |
| description |
The aim of this study was to characterize the protein profile of ovarian follicular fluid (FF) of brown brocket deer (Mazama gouazoubira). Five adult females received an ovarian stimulation treatment and the FF was collected by laparoscopy from small/medium (<= 3.5 mm) and large (>3.5 mm) follicles. Concentrations of soluble proteins in FF samples were measured and proteins were analyzed by 1-D SDS-PAGE followed by tryptic digestion and tandem mass spectrometry. Data from protein list defined after a Mascot database search were analyzed using the STRAP software tool. For the protein concentration, no significant difference (P> 0.05) was observed between small/medium and large follicles: 49.2 +/- 22.8 and 56.7 +/- 27.4 mu g/mu l, respectively. Mass spectrometry analysis identified 13 major proteins, but with no significant difference (P> 0.05) between follicle size class. This study provides insight into elucidating folliculogenesis in brown brocket deer. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-04-01 2021-06-25T11:23:13Z 2021-06-25T11:23:13Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1017/S0967199419000741 Zygote. New York: Cambridge Univ Press, v. 28, n. 2, p. 170-173, 2020. 0967-1994 http://hdl.handle.net/11449/208868 10.1017/S0967199419000741 WOS:000578224800012 |
| url |
http://dx.doi.org/10.1017/S0967199419000741 http://hdl.handle.net/11449/208868 |
| identifier_str_mv |
Zygote. New York: Cambridge Univ Press, v. 28, n. 2, p. 170-173, 2020. 0967-1994 10.1017/S0967199419000741 WOS:000578224800012 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
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Zygote |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.format.none.fl_str_mv |
170-173 |
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Cambridge Univ Press |
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Cambridge Univ Press |
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Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1799965434445824000 |