Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3923/jbs.2006.596.603 http://hdl.handle.net/11449/68875 |
Resumo: | The aim of this study was to examine the role of nifedipine and Nitric Oxide (NO) on salivary flow and compounds (salivary amylase, saliva total proteins, saliva calcium, sodium and potassium). Male Holtzman rats weighting 200-250 g were anesthetized with zoletil 50 mg kg -1 (tiletamine chloridrate 125.0 mg and zolazepan chloridrate 125.0 mg) into quadriceps muscle and stainless steel cannulas were implanted into their lateral ventricle of the brain (LV). Animals in divided group were injected with nifedipine (50 μg μL -1) alone and in combination with 7-nitroindazol (7-NIT) (40 μg μL -1), neuronal NO Sinthase Inhibitor (nNOSI) and Sodium Nitroprussate (SNP) (30 μg μL -1) NO donor agent. As a secretory stimuli, pilocarpine dissolved in isotonic was administered intraperitoneally (ip) at a dosage of 10 mg kg -1 body weight. Saliva was collected for 7 min with four cotton balls weighing approximately 20 mg each, two of which were placed on either side of the oral cavity, with the other two placed under the tongue. Nifedipine treatment induced a reduction in saliva secretion rate and concentration of amylase, total protein and calcium without changes in sodium and potassium concentration in comparison with controls. Co-treatment of animals with nifedipine and SNP retained flow rate and concentration of amylase, total protein and calcium in normal levels. Co-treatment of animals with nifedipine and 7-NIT potentiated the effect of nifedipine on the reduction of saliva secretion and concentrations of amylase, total protein and calcium. Nifedipine (dihydroperidine) calcium-channel blocker widely in use is associated with salivary dysfunction acting in the central nervous system structures. NO might be the mechanism for protective effect against the nifedipine-induce salivary dysfunction, acting in the CNS. © 2006 Asian Network for Scientific Information. |
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Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxideBlood pressureLateral ventricleNifedipineNitric oxideSalivaWater intake7 nitroindazoleamylasecalciumisotonic solutionnifedipinenitric oxidenitric oxide donornitric oxide synthase inhibitornitroprusside sodiumpilocarpinepotassiumsaliva proteinsodiumstainless steeltelazolanalysis of varianceanimal experimentanimal modelanimal tissuearterial pressurebody weightcontrolled studydrug potentiationflow ratefluid intakehistopathologylateral brain ventriclemalenonhumanquadriceps femoris muscleratsalivation disorderAnimaliaGossypium hirsutumThe aim of this study was to examine the role of nifedipine and Nitric Oxide (NO) on salivary flow and compounds (salivary amylase, saliva total proteins, saliva calcium, sodium and potassium). Male Holtzman rats weighting 200-250 g were anesthetized with zoletil 50 mg kg -1 (tiletamine chloridrate 125.0 mg and zolazepan chloridrate 125.0 mg) into quadriceps muscle and stainless steel cannulas were implanted into their lateral ventricle of the brain (LV). Animals in divided group were injected with nifedipine (50 μg μL -1) alone and in combination with 7-nitroindazol (7-NIT) (40 μg μL -1), neuronal NO Sinthase Inhibitor (nNOSI) and Sodium Nitroprussate (SNP) (30 μg μL -1) NO donor agent. As a secretory stimuli, pilocarpine dissolved in isotonic was administered intraperitoneally (ip) at a dosage of 10 mg kg -1 body weight. Saliva was collected for 7 min with four cotton balls weighing approximately 20 mg each, two of which were placed on either side of the oral cavity, with the other two placed under the tongue. Nifedipine treatment induced a reduction in saliva secretion rate and concentration of amylase, total protein and calcium without changes in sodium and potassium concentration in comparison with controls. Co-treatment of animals with nifedipine and SNP retained flow rate and concentration of amylase, total protein and calcium in normal levels. Co-treatment of animals with nifedipine and 7-NIT potentiated the effect of nifedipine on the reduction of saliva secretion and concentrations of amylase, total protein and calcium. Nifedipine (dihydroperidine) calcium-channel blocker widely in use is associated with salivary dysfunction acting in the central nervous system structures. NO might be the mechanism for protective effect against the nifedipine-induce salivary dysfunction, acting in the CNS. © 2006 Asian Network for Scientific Information.Basic Institute of Biosciences UNITAU, Taubaté, SPDepartment of Biological and Health Science UNIARA, Araraquara, SPDepartment of Physiology and Pathology School of Dentistry Paulista State University, 1680 Humaitá Street, Araraquara, SP 14801-903Department of Anesthesiology Clinic Hospital State of São Paulo, São PauloDepartment of Physiology Federal University of São Carlos SP, São PauloDepartment of Gastroenterology Clinic Hospital State of São Paulo, São PauloDepartment of Physiology and Pathology School of Dentistry Paulista State University, 1680 Humaitá Street, Araraquara, SP 14801-903Universidade de Taubaté (UNITAU)Centro Universitário de Araraquara (UNIARA)Universidade Estadual Paulista (Unesp)Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP)Universidade Federal de São Carlos (UFSCar)Saad, Wilson Abrão [UNESP]Guarda, Ismael Francisco Motta SiqueiraCamargo, Luiz Antonio de Arruda [UNESP]Santos, Talmir Augusto Faria Brizola dosSimões, SylvioSaad, William Abrão2014-05-27T11:21:52Z2014-05-27T11:21:52Z2006-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article596-603http://dx.doi.org/10.3923/jbs.2006.596.603Journal of Biological Sciences, v. 6, n. 3, p. 596-603, 2006.1727-30481812-5719http://hdl.handle.net/11449/6887510.3923/jbs.2006.596.6032-s2.0-33745769561Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Biological Sciences0,1740,174info:eu-repo/semantics/openAccess2021-10-22T17:43:20Zoai:repositorio.unesp.br:11449/68875Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-22T17:43:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide |
title |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide |
spellingShingle |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide Saad, Wilson Abrão [UNESP] Blood pressure Lateral ventricle Nifedipine Nitric oxide Saliva Water intake 7 nitroindazole amylase calcium isotonic solution nifedipine nitric oxide nitric oxide donor nitric oxide synthase inhibitor nitroprusside sodium pilocarpine potassium saliva protein sodium stainless steel telazol analysis of variance animal experiment animal model animal tissue arterial pressure body weight controlled study drug potentiation flow rate fluid intake histopathology lateral brain ventricle male nonhuman quadriceps femoris muscle rat salivation disorder Animalia Gossypium hirsutum |
title_short |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide |
title_full |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide |
title_fullStr |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide |
title_full_unstemmed |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide |
title_sort |
Central nifedipine-induced alterations in salivary flow and compounds: Role of nitric oxide |
author |
Saad, Wilson Abrão [UNESP] |
author_facet |
Saad, Wilson Abrão [UNESP] Guarda, Ismael Francisco Motta Siqueira Camargo, Luiz Antonio de Arruda [UNESP] Santos, Talmir Augusto Faria Brizola dos Simões, Sylvio Saad, William Abrão |
author_role |
author |
author2 |
Guarda, Ismael Francisco Motta Siqueira Camargo, Luiz Antonio de Arruda [UNESP] Santos, Talmir Augusto Faria Brizola dos Simões, Sylvio Saad, William Abrão |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de Taubaté (UNITAU) Centro Universitário de Araraquara (UNIARA) Universidade Estadual Paulista (Unesp) Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Saad, Wilson Abrão [UNESP] Guarda, Ismael Francisco Motta Siqueira Camargo, Luiz Antonio de Arruda [UNESP] Santos, Talmir Augusto Faria Brizola dos Simões, Sylvio Saad, William Abrão |
dc.subject.por.fl_str_mv |
Blood pressure Lateral ventricle Nifedipine Nitric oxide Saliva Water intake 7 nitroindazole amylase calcium isotonic solution nifedipine nitric oxide nitric oxide donor nitric oxide synthase inhibitor nitroprusside sodium pilocarpine potassium saliva protein sodium stainless steel telazol analysis of variance animal experiment animal model animal tissue arterial pressure body weight controlled study drug potentiation flow rate fluid intake histopathology lateral brain ventricle male nonhuman quadriceps femoris muscle rat salivation disorder Animalia Gossypium hirsutum |
topic |
Blood pressure Lateral ventricle Nifedipine Nitric oxide Saliva Water intake 7 nitroindazole amylase calcium isotonic solution nifedipine nitric oxide nitric oxide donor nitric oxide synthase inhibitor nitroprusside sodium pilocarpine potassium saliva protein sodium stainless steel telazol analysis of variance animal experiment animal model animal tissue arterial pressure body weight controlled study drug potentiation flow rate fluid intake histopathology lateral brain ventricle male nonhuman quadriceps femoris muscle rat salivation disorder Animalia Gossypium hirsutum |
description |
The aim of this study was to examine the role of nifedipine and Nitric Oxide (NO) on salivary flow and compounds (salivary amylase, saliva total proteins, saliva calcium, sodium and potassium). Male Holtzman rats weighting 200-250 g were anesthetized with zoletil 50 mg kg -1 (tiletamine chloridrate 125.0 mg and zolazepan chloridrate 125.0 mg) into quadriceps muscle and stainless steel cannulas were implanted into their lateral ventricle of the brain (LV). Animals in divided group were injected with nifedipine (50 μg μL -1) alone and in combination with 7-nitroindazol (7-NIT) (40 μg μL -1), neuronal NO Sinthase Inhibitor (nNOSI) and Sodium Nitroprussate (SNP) (30 μg μL -1) NO donor agent. As a secretory stimuli, pilocarpine dissolved in isotonic was administered intraperitoneally (ip) at a dosage of 10 mg kg -1 body weight. Saliva was collected for 7 min with four cotton balls weighing approximately 20 mg each, two of which were placed on either side of the oral cavity, with the other two placed under the tongue. Nifedipine treatment induced a reduction in saliva secretion rate and concentration of amylase, total protein and calcium without changes in sodium and potassium concentration in comparison with controls. Co-treatment of animals with nifedipine and SNP retained flow rate and concentration of amylase, total protein and calcium in normal levels. Co-treatment of animals with nifedipine and 7-NIT potentiated the effect of nifedipine on the reduction of saliva secretion and concentrations of amylase, total protein and calcium. Nifedipine (dihydroperidine) calcium-channel blocker widely in use is associated with salivary dysfunction acting in the central nervous system structures. NO might be the mechanism for protective effect against the nifedipine-induce salivary dysfunction, acting in the CNS. © 2006 Asian Network for Scientific Information. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-05-01 2014-05-27T11:21:52Z 2014-05-27T11:21:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3923/jbs.2006.596.603 Journal of Biological Sciences, v. 6, n. 3, p. 596-603, 2006. 1727-3048 1812-5719 http://hdl.handle.net/11449/68875 10.3923/jbs.2006.596.603 2-s2.0-33745769561 |
url |
http://dx.doi.org/10.3923/jbs.2006.596.603 http://hdl.handle.net/11449/68875 |
identifier_str_mv |
Journal of Biological Sciences, v. 6, n. 3, p. 596-603, 2006. 1727-3048 1812-5719 10.3923/jbs.2006.596.603 2-s2.0-33745769561 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Biological Sciences 0,174 0,174 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
596-603 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965365019607040 |