Canine visceral leishmaniasis in the Northeast Region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s40409-016-0069-4 http://hdl.handle.net/11449/172817 |
Resumo: | Background: Visceral leishmaniasis (VL) is a zoonosis that affects dogs and other mammals, including humans. Contact with dogs is a major risk factor for humans. This disease is endemic in several regions of Brazil. The aim of this study was to determine the prevalence of Leishmania spp. infection in dogs and to correlate it with possible risk factors. Methods: Blood samples were collected from 391 dogs of different ages, breeds, and both genders, coming from Campina Grande, Paraíba state, Brazil. An epidemiological questionnaire was employed in order to identify risk factors associated with the disease. Serological tests were performed using indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA S7®) and polymerase chain reaction. Results: Leishmania spp. antibodies were detected in 33 (8.4 %) and 17 (4.3 %) dogs according to the indirect immunofluorescence test (IFAT) and enzyme-linked immunosorbent assay (ELISA S7®), respectively. PCR results indicated the presence of L. chagasi DNA in only eight (2 %) blood samples. There was a significant association between reactive animals and contact with animals from different houses (OR = 4.1; p = 0.02). Conclusions: It is suggested that CVL may occur in urban areas. Moreover, it is demonstrated that the association among different diagnostic tests may lead to a more accurate identification of positive animals, which might help to improve the disease control and prevent euthanasia in false-positive results. |
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Canine visceral leishmaniasis in the Northeast Region of BrazilDiagnosisDogKala-azarRisk factorsZoonosisBackground: Visceral leishmaniasis (VL) is a zoonosis that affects dogs and other mammals, including humans. Contact with dogs is a major risk factor for humans. This disease is endemic in several regions of Brazil. The aim of this study was to determine the prevalence of Leishmania spp. infection in dogs and to correlate it with possible risk factors. Methods: Blood samples were collected from 391 dogs of different ages, breeds, and both genders, coming from Campina Grande, Paraíba state, Brazil. An epidemiological questionnaire was employed in order to identify risk factors associated with the disease. Serological tests were performed using indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA S7®) and polymerase chain reaction. Results: Leishmania spp. antibodies were detected in 33 (8.4 %) and 17 (4.3 %) dogs according to the indirect immunofluorescence test (IFAT) and enzyme-linked immunosorbent assay (ELISA S7®), respectively. PCR results indicated the presence of L. chagasi DNA in only eight (2 %) blood samples. There was a significant association between reactive animals and contact with animals from different houses (OR = 4.1; p = 0.02). Conclusions: It is suggested that CVL may occur in urban areas. Moreover, it is demonstrated that the association among different diagnostic tests may lead to a more accurate identification of positive animals, which might help to improve the disease control and prevent euthanasia in false-positive results.São Paulo State University (UNESP - Univ Estadual Paulista)Mississippi State University of Agriculture and Applied ScienceFederal University of PernambucoFederal University of Campina GrandeSão Paulo State University (UNESP - Univ Estadual Paulista) Diagnostic Service in Zoonosis Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Husbandry, District of Rubião Junior, s/nSão Paulo State University (UNESP - Univ Estadual Paulista)São Paulo State University (UNESP - Univ Estadual Paulista) Diagnostic Service in Zoonosis Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Husbandry, District of Rubião Junior, s/nUniversidade Estadual Paulista (Unesp)Mississippi State University of Agriculture and Applied ScienceUniversidade Federal de Pernambuco (UFPE)Federal University of Campina GrandeBrito, Flávio Gonçalves [UNESP]Langoni, Helio [UNESP]Silva, Rodrigo Costa daRotondano, Tereza Emmanuelle de FariasMelo, Marcia Almeida deda Paz, Giselle Souza [UNESP]2018-12-11T17:02:17Z2018-12-11T17:02:17Z2016-04-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s40409-016-0069-4Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 22, n. 1, 2016.1678-91991678-9180http://hdl.handle.net/11449/17281710.1186/s40409-016-0069-4S1678-919920160001003112-s2.0-84963613070S1678-91992016000100311.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases0,573info:eu-repo/semantics/openAccess2023-11-07T06:08:42Zoai:repositorio.unesp.br:11449/172817Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-07T06:08:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Canine visceral leishmaniasis in the Northeast Region of Brazil |
title |
Canine visceral leishmaniasis in the Northeast Region of Brazil |
spellingShingle |
Canine visceral leishmaniasis in the Northeast Region of Brazil Brito, Flávio Gonçalves [UNESP] Diagnosis Dog Kala-azar Risk factors Zoonosis |
title_short |
Canine visceral leishmaniasis in the Northeast Region of Brazil |
title_full |
Canine visceral leishmaniasis in the Northeast Region of Brazil |
title_fullStr |
Canine visceral leishmaniasis in the Northeast Region of Brazil |
title_full_unstemmed |
Canine visceral leishmaniasis in the Northeast Region of Brazil |
title_sort |
Canine visceral leishmaniasis in the Northeast Region of Brazil |
author |
Brito, Flávio Gonçalves [UNESP] |
author_facet |
Brito, Flávio Gonçalves [UNESP] Langoni, Helio [UNESP] Silva, Rodrigo Costa da Rotondano, Tereza Emmanuelle de Farias Melo, Marcia Almeida de da Paz, Giselle Souza [UNESP] |
author_role |
author |
author2 |
Langoni, Helio [UNESP] Silva, Rodrigo Costa da Rotondano, Tereza Emmanuelle de Farias Melo, Marcia Almeida de da Paz, Giselle Souza [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Mississippi State University of Agriculture and Applied Science Universidade Federal de Pernambuco (UFPE) Federal University of Campina Grande |
dc.contributor.author.fl_str_mv |
Brito, Flávio Gonçalves [UNESP] Langoni, Helio [UNESP] Silva, Rodrigo Costa da Rotondano, Tereza Emmanuelle de Farias Melo, Marcia Almeida de da Paz, Giselle Souza [UNESP] |
dc.subject.por.fl_str_mv |
Diagnosis Dog Kala-azar Risk factors Zoonosis |
topic |
Diagnosis Dog Kala-azar Risk factors Zoonosis |
description |
Background: Visceral leishmaniasis (VL) is a zoonosis that affects dogs and other mammals, including humans. Contact with dogs is a major risk factor for humans. This disease is endemic in several regions of Brazil. The aim of this study was to determine the prevalence of Leishmania spp. infection in dogs and to correlate it with possible risk factors. Methods: Blood samples were collected from 391 dogs of different ages, breeds, and both genders, coming from Campina Grande, Paraíba state, Brazil. An epidemiological questionnaire was employed in order to identify risk factors associated with the disease. Serological tests were performed using indirect immunofluorescence, enzyme-linked immunosorbent assay (ELISA S7®) and polymerase chain reaction. Results: Leishmania spp. antibodies were detected in 33 (8.4 %) and 17 (4.3 %) dogs according to the indirect immunofluorescence test (IFAT) and enzyme-linked immunosorbent assay (ELISA S7®), respectively. PCR results indicated the presence of L. chagasi DNA in only eight (2 %) blood samples. There was a significant association between reactive animals and contact with animals from different houses (OR = 4.1; p = 0.02). Conclusions: It is suggested that CVL may occur in urban areas. Moreover, it is demonstrated that the association among different diagnostic tests may lead to a more accurate identification of positive animals, which might help to improve the disease control and prevent euthanasia in false-positive results. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-04-12 2018-12-11T17:02:17Z 2018-12-11T17:02:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s40409-016-0069-4 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 22, n. 1, 2016. 1678-9199 1678-9180 http://hdl.handle.net/11449/172817 10.1186/s40409-016-0069-4 S1678-91992016000100311 2-s2.0-84963613070 S1678-91992016000100311.pdf |
url |
http://dx.doi.org/10.1186/s40409-016-0069-4 http://hdl.handle.net/11449/172817 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 22, n. 1, 2016. 1678-9199 1678-9180 10.1186/s40409-016-0069-4 S1678-91992016000100311 2-s2.0-84963613070 S1678-91992016000100311.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1792961756518154240 |